Near-Field Terahertz Nanoscopy Spatially Resolves Chiral Drug-Cell Interactions: Toward Precision Intracellular Pharmacology [0.03%]
近场太赫兹纳米显微镜空间分辨手性药物-细胞相互作用：迈向精确的细胞内药理学
Xitian Hu,Yadi Wang,Yue Wang et al.
Xitian Hu et al.
Chiral drug enantiomers frequently exhibit marked differences in pharmacological efficacy and toxicity profiles. However, the resolution limitations of traditional detection methodologies render them incapable of resolving the spatial heter...
Bimodal Electrochemiluminescence of Ru-MOFs in the Presence of Double Coreactants for CEA Detection [0.03%]
双核心反应剂存在下Ru-MOFs电致化学发光的双峰模式及CEA检测
Xiaomei Huang,Xiang Deng,Zihe Deng et al.
Xiaomei Huang et al.
A dual-potential electrochemiluminescence (ECL) immunosensor, featuring simultaneous cathodic and anodic emission from ruthenium-based metal-organic frameworks (Ru-MOFs) as the sole luminophore and iron-monatomic catalysts (FeNC-SN SACs) wi...
Unveiling the Spatiotemporal Behavior of Chiral Quantum Dots During Cellular Endocytosis [0.03%]
细胞内吞作用过程中手性量子点的时空行为研究
Shoupeng Wang,Weiheng Kong,Bangming Wang et al.
Shoupeng Wang et al.
The endocytosis of chiral quantum dots is the key initial step in their interaction with biological systems, and it significantly influences subsequent biological effects. In this study, we delve into the mechanism of chiral quantum dots du...
Antibody-Directed Cell Internalization of Targeted Covalent Europium Tag Enables In Situ Kinase Labeling and Inductively Coupled Plasma Mass Spectrometry (ICP-MS) Quantification [0.03%]
抗体介导的靶向铕标记物的细胞内吞作用使原位激酶标记和电感耦合等离子体质谱法（ICP-MS）定量成为可能
Ziyang Fang,Xinyue Zhao,Ruxue Hou et al.
Ziyang Fang et al.
Development of methods to accurately detect the expression and activity of protein targets inside live cells is of great significance for disease diagnostics and drug development. Inductively coupled plasma mass spectrometry (ICP-MS) has be...
Lanthanide Complexes-Doped Silica Nanoparticles for Dual-Modal Ratiometric Time-Gated Luminescence/Magnetic Resonance Imaging of Inflammatory Diseases [0.03%]
用于炎症疾病诊疗的掺杂镧系金属配合物的时间门控比率型发光/磁共振成像二氧化硅纳米粒子
Hua Ma,Qi Liu,Eman A Akam-Baxter et al.
Hua Ma et al.
Inflammation plays a pivotal role in various diseases, and a key biomarker of inflammation is hypochlorous acid (HClO), an upregulated reactive species that can cause extensive tissue damage. Accurate detection of HClO is essential for enha...
Spherical Nucleic Acids as Modulators of CRISPR/Cas12a by a Steric Barrier Effect for Designing Versatile Biosensors [0.03%]
基于立体屏障效应调节CRISPR / Cas12a的球形核酸设计多功能生物传感器
Fengxi Zhu,Qiang Zhao
Fengxi Zhu
Discovery of CRISPR/Cas12a has revolutionized broad fields, including gene editing, molecular diagnosis, and biosensing. Flexible regulation of Cas12a activity is important for diverse CRISPR/Cas12a applications, especially for biosensing, ...
Label-Free Immunohistochemistry Imaging by Oblique-Incidence Reflectivity Difference [0.03%]
倾斜入射反射差法无标记免疫组化成像
Meng Li,Yuanyuan Xie,Dandan Ji et al.
Meng Li et al.
Immunohistochemistry (IHC) is a well-established method in tissue pathology and finds critical applications in both clinical and fundamental medical science. Generally, labeled antibodies are a requisite for IHC to visualize the spatial dis...
Concentration-Tunable Catalytic Strand-Driven Fast Self-Reinforcing Circular DNA Nanoamplifier under Precise Activation of NIR Light Guidance for Sensitive Fluorescence Imaging [0.03%]
浓度可调的催化引发的发夹作用驱动的快速自增强式环形DNA纳米放大器在近红外光精确激活下用于敏感荧光成像
Jingwei Qiu,Zhuo Chen,Qing Tang et al.
Jingwei Qiu et al.
Although there is great promise in cascaded DNA nanoamplifiers for fluorescence imaging, several key hurdles (e.g., insufficient sensitivity, suboptimal reaction kinetics, and unsatisfactory detection precision) should be further addressed....
PEG Precipitation Followed by Albumin Depletion for Plasma Proteomics Analysis [0.03%]
聚乙二醇沉淀结合脱白蛋白法用于血浆蛋白质组学分析
Yanyang Wang,Minqi Cai,Yuning Song et al.
Yanyang Wang et al.
The mass spectrometry (MS)-based blood plasma or serum proteomic analysis is limited by interference from albumin, immunoglobins, and other highly abundant proteins. We have found that poly(ethylene glycol) (PEG) can efficiently precipitate...
Exploring the Use of Nanoporous Glass Substrates for the Quantitative Trace Microanalysis of Aqueous Samples by Surface-Enhanced Laser-Induced Breakdown Spectroscopy [0.03%]
用于水溶液样品表面增强激光诱导击穿光谱定量痕量微分析的纳米多孔玻璃基底的研究
Gyula Kajner,Gábor Galbács
Gyula Kajner
In this study, a novel laser-induced breakdown spectroscopy (LIBS) liquid sample presentation method is showcased based on drying microdroplets (2-3 μL) of aqueous solutions on nanoporous borosilicate glass (NPG) substrates possessing an a...