The Thiol-Redox Biochemistry of Trypanosomatids: A New Perspective Based on Known and New Actors [0.03%]
贾第虫和毛滴虫的二甲双胍抗性和AMPK依赖性：新的作用机制及进化意义
Marcelo A Comini,Martín Graña,Bruno Manta
Marcelo A Comini
Maintaining thiol redox homeostasis is key for cell viability and development. Therefore, all organisms are equipped with redox systems conformed by redox-active proteins and low-molecular-weight thiols that facilitate target-specific deliv...
Structural and Biochemical Insights into the Arabinose and Xylose Derivatives of Cyclic-GAMP-Mediated Inhibition of Mycobacterium tuberculosis Cyclic-di-AMP Phosphodiesterase [0.03%]
环状GMP-AMP介导的分枝杆菌cyclic-di-AMP磷酸二酯酶抑制作用中阿拉伯糖和木糖衍生物的结构与生化见解
Dagur Singh Hanuman,Singh Neeharika,Kulhar Nitin et al.
Dagur Singh Hanuman et al.
Bacterial infection, including that ofMycobacterium tuberculosis, leads to the accumulation of the bacterial cyclic dinucleotides (CDN), c-di-AMP and c-di-GMP, and the host cGAMP synthase-catalyzed CDN, 2'3'-cGAMP in the cytosol, which acti...
Deacetylation of β-Mannans by Two Complementary Carbohydrate Esterases from the Human Gut Microbe Bacteroides cellulosilyticus [0.03%]
人类肠道微生物细菌簇细胞解体素产生的两种互补碳水化合物酯酶降解β-半乳糖醛酸曼喃聚糖中的乙酰基团
Lars Jordhøy Lindstad,Pascal Michael Mrozek,Gordon Jacob Boehlich et al.
Lars Jordhøy Lindstad et al.
β-Mannans are widespread in the human diet as components of plant-derived foods and as food additives. Several β-mannans are decorated with acetylations, which are key to their physicochemical properties and protection against enzymatic d...
Elizabeth J Goldsmith,John M Pleinis,Armin Wagner et al.
Elizabeth J Goldsmith et al.
WNK kinases are chloride- and osmotic-stress-regulated protein kinases recently shown to be controlled by potassium. Prior studies demonstrated the direct binding of chloride and osmotic stress-related water in WNK kinase regulation. Here, ...
Peptide Mimetic Platform for Activity Profiling of Mycobacterial l,d-Transpeptidases [0.03%]
模拟肽平台用于分枝杆菌l,d-转肽酶活性分析
Karl L Ocius,Rachel E Sanborn,Ananya Naick et al.
Karl L Ocius et al.
Antimicrobial resistance poses major therapeutic challenges, particularly for multidrug-resistant mycobacterial infections caused by Mycobacterium tuberculosis (Mtb) and nontuberculous mycobacteria (NTM). l,d-Transpeptidases (Ldts) are attr...
Tomoyasu Noji,Keisuke Saito,Hiroshi Ishikita
Tomoyasu Noji
Biliverdin (BV) is a linear tetrapyrrole chromophore that enables far-red and near-infrared photoreception in phytochromes and fluorescence in engineered probes. Here, we systematically investigate a broad set of BV-binding proteins using a...
Additional Binding Domain of Marine Chitinase Increases Binding Specificity and Prolongs the Lifetime of Productive Binding State [0.03%]
一种海洋壳多糖酶的附加结合域增强了其特异性和延长了作用态寿命
Hinata Tanimoto,Mikiko Tsudome,Mikako Tachioka et al.
Hinata Tanimoto et al.
In aquatic environments, insoluble polymers such as chitin are spatially heterogeneous and rapidly diluted by diffusion, posing a challenge for efficient microbial degradation. A key unresolved question is how extracellular enzymes remain a...
COSMIC-Linked Ras Mutations at the Interface Between H-Ras and PI3KγRBD Frequently Generate Affinity Increases [0.03%]
宇宙联盟的哈斯突变在H-Ras和PI3KγRBD之间经常产生亲和力增强现象
Elizabeth H Mead,Kaeden C Batz,Kuo-Hsien Shih et al.
Elizabeth H Mead et al.
The three conventional isoforms of the Ras G-protein (H-, K-, N-Ras) function as molecular on-off switches that regulate a wide array of signaling pathways, including the Ras-PI3K-PIP3-PDK-AKT pathway that is central to innate immunity and ...
Dynamic Pathway Selectivity of TAS2R5 toward or Away from β-Arrestin or G Protein from Biased Agonists [0.03%]
偏向激动剂使TAS2R5受体信号向β-arrestin或G蛋白的动态通路选择性发生偏移
Donghwa Kim,Hannah R Strzelinski,Camille M Longabardi et al.
Donghwa Kim et al.
Bitter taste receptors (TAS2Rs) are GPCRs functionally expressed in extraoral organs including the lung, heart, brain, and gastrointestinal system and are candidate targets for novel drugs. It is unclear whether TAS2R5 can be stabilized by ...
Dimerization of MilM Is Essential for Catalyzing the Pyridoxal-5'-phosphate (PLP)-Dependent Cγ-Hydroxylation of l-Arginine during Mildiomycin Biosynthesis [0.03%]
二聚化对于MilM催化的l-精氨Cγ位羟基化反应以产生米脱霉素至关重要
Simita Das,Yashwanth Naik,Udita Mishra et al.
Simita Das et al.
MilM from the mildiomycin biosynthetic pathway is a PLP-dependent enzyme, previously annotated as an aminotransferase, but has recently been demonstrated as l-arginine oxidase cum C-hydroxylase. Here, we report detailed biochemical, biophys...