Spontaneous Refolding of Escherichia coli Methionine Adenosyltransferase (MetK) Is Extremely Slow, Indicative of Chaperone Dependence [0.03%]
大肠杆菌蛋氨酸腺苷转移酶(MetK)的自发重折叠极为缓慢，提示其依赖分子伴侣辅助折叠
Anupam Barai,Pronoy Majumder,Madhurima Sarkar et al.
Anupam Barai et al.
Escherichia coli methionine adenosyltransferase (MetK) catalyzes the synthesis of S-adenosylmethionine from ATP and l-methionine and is essential for cellular metabolism. To gain insight into its conformational stability and folding behavio...
Mena EVH1 Interacts with PTP1B Via a Distributed Interface Without Regulating Enzymatic Activity [0.03%]
Mena EVH1 域通过分散型界面与 PTP1B 相互作用但不调控其酶活性
Lanette LaComb,Sean M Cahill,Jeffrey B Bonanno et al.
Lanette LaComb et al.
The interaction between mammalian enabled protein (Mena), an actin regulatory protein, and protein tyrosine phosphatase 1B (PTP1B) is critical for maintaining epidermal growth factor receptor (EGFR) signaling homeostasis and regulating cell...
Development of a FRET-Based Assay for Human Neutral Sphingomyelinase 2 [0.03%]
人类中性鞘磷脂酶2的FRET检测方法的建立
Khalayi Martha Aywa,Christian Kappe,Botheina Ghandour et al.
Khalayi Martha Aywa et al.
Neutral sphingomyelinase 2 (nSMase2) is a membrane-bound enzyme that hydrolyzes sphingomyelin (SM) into ceramide and phosphocholine. By generating the bioactive lipid ceramide, nSMase2 plays a critical role in cell stress responses and in r...
Suppression of Thermal Protein Aggregation by Acetylation of Lysine Side Chains [0.03%]
乙酰化赖氨酸侧链通过抑制热变性过程中的蛋白质聚集来稳定蛋白质
Yuen Ki Ng,Lars Konermann
Yuen Ki Ng
The association of proteins into amorphous aggregates is linked to various detrimental phenomena, ranging from disease mechanisms to the degradation of biopharmaceuticals. The development of aggregation suppression strategies is of consider...
A Pair of FAD-Dependent Carbohydrate Oxidases from the Corn Smut Pathogen Ustilago maydis Oxidize Chitosan Oligomers [0.03%]
玉米锈赤霉中一对由FAD依赖的碳水化合物氧化酶氧化壳聚糖低聚物
Roseline Assiah Yao,Simone Turella,Sanchari Banerjee et al.
Roseline Assiah Yao et al.
Auxiliary activity family 7 (AA7) oxidoreductases are fungal flavoenzymes that catalyze the C1 oxidation of diverse oligosaccharides coupled to the reduction of molecular oxygen (oxidase activity) or organic molecules (dehydrogenase activit...
Energetic Determinants of Expanded PAM Recognition in Engineered FnCas9 [0.03%]
工程化FnCas9扩增PAM识别的能控因素
Ajit Kumar,Priyanka Kumari,Shalini Mishra et al.
Ajit Kumar et al.
Expanding protospacer-adjacent motif (PAM) compatibility while preserving specificity remains a central challenge in CRISPR-Cas9 engineering. Francisella novicida Cas9 (FnCas9) exhibits high intrinsic specificity but is constrained by strin...
Direct Binding of Cysteine-367 Thiolate to the Active Site of the [FeFe]-Hydrogenase from Clostridium beijerinckii in the O2-Stable State [0.03%]
缺氧状态下关于产氢酶铁铁辅基与半胱氨酸残基直接结合的证据：来自贝杰林克芽孢杆菌的一个例子
Jifu Duan,Federica Arrigoni,Andreas Rutz et al.
Jifu Duan et al.
[FeFe]-hydrogenases are very active biocatalysts for H2 conversion. However, their active site is vulnerable to irreversible degradation initiated by O2 binding at the catalytic iron ion (Fed) of the active center. CbA5H, the [FeFe]-hydroge...
Jiro Karlo,Dhananjaya Giriraju,Nilkamal Mahanta et al.
Jiro Karlo et al.
Obtaining biochemical information and monitoring the biomolecules in complex biological systems and environments remains a challenge and requires methods that can detect biomolecules. Raman spectroscopy has emerged as a powerful analytical ...
Fluorogenic RNA Aptamer-Based Activity Quantification of T7 RNA Polymerase [0.03%]
荧光RNA适配体法表征T7 RNA聚合酶活性
Khalil Mimouni,Weiqi Qiu,Huiqing Zhou
Khalil Mimouni
T7 RNA polymerase (T7 RNAP) is a central molecular biology tool that is broadly used for biomedical research and the RNA therapeutic industry. T7 RNAP and its engineered variants provide valuable tools in synthetic biology and gene expressi...
Back to the Future of Biochemistry: Recent Advances from Past Contributors [0.03%]
重温生物化学的未来：过去贡献者的最新进展
Ruchi Anand,Jeremy M Baskin
Ruchi Anand