Sweet Protein Allosteric Binding and Activation of the Human T1R2/R3 Sweet Receptor: A Simulation Model Validated by in Vitro Receptor Activation Assay [0.03%]
天冬甜蛋白别构结合并激活人T1R2/T1R3甜味受体：一种经体外受体激活实验验证的模拟模型
Phillip Vo,Daniel E Connors,Brendan P Sharkey et al.
Phillip Vo et al.
Sweet proteins trigger sweet taste perception through interactions with the human T1R2/R3 sweet taste receptor. To date, relatively few proteins have been identified as causing sweet taste perception, and the four most studied proteins: mon...
Mapping RNA-Binding Proteins on the Ribosome by Tethered Micrococcal Nuclease [0.03%]
通过拴系的微coccal核酸酶在核糖体上绘制RNA结合蛋白
Chia Yi Yao,Simpson Joseph
Chia Yi Yao
RNA-binding proteins (RBPs) are essential regulators of posttranscriptional gene expression, influencing mRNA processing, translation, and stability. Defining their binding sites on RNA is key to understanding how they assemble into functio...
Investigating Enzyme-Substrate Interactions in the Assembly of K48/K63 Heterotypic Ubiquitin Chains [0.03%]
关于K48/K63异型泛素链组装中酶-底物相互作用的探究
Gajendra Singh,Hitendra Negi,Aravind Ravichandran et al.
Gajendra Singh et al.
Ubiquitination, a post-translational modification, regulates numerous cellular processes by attaching ubiquitin molecules to the target proteins, thereby altering their cellular levels and functions. A central aspect of ubiquitin-mediated s...
Cyclized Peptide Inhibitors of the Small G Protein Cdc42 Mimic Binding of Effector Proteins [0.03%]
模拟效应蛋白结合的环化肽类Cdc42小G蛋白抑制剂
Natasha P Murphy,George J N Tetley,Jefferson Revell et al.
Natasha P Murphy et al.
The Ras superfamily of small GTPases are challenging targets for therapeutic inhibition, partially due to a lack of pockets amenable to small molecule inhibition. Our previous work identified high-affinity cyclized peptide binders of Cdc42,...
Catriona Robinson,Michael Yuen,Harry Brough et al.
Catriona Robinson et al.
Quantum mechanical tunneling (QMT) is now recognized as a significant contributor to some enzyme catalyzed hydrogen transfer reactions. In this perspective, we examine recent theoretical and experimental advances that investigate when, and ...
Influence of the Electrostatic Tuning of Alkyl and Benzyl Groups on Cation-π Interactions in Histone Reader Proteins and β-Hairpin Peptides [0.03%]
烷基和苯甲基电荷调节对组蛋白阅读器蛋白质及β发卡肽中阳离子-π相互作用的影响研究
Meghan N Ricciardi,Christopher R Travis,Joseph W Treacy et al.
Meghan N Ricciardi et al.
Reader proteins that bind histone trimethyllysine (Kme3) have become active therapeutic targets in recent years. Binding of Kme3 in the conserved aromatic cages of these readers via cation-π interactions is a key contributor to these prote...
Screening of FDA-Approved Small Molecules to Discover Inhibitors of the Pseudomonas aeruginosa Quorum-Sensing Enzyme, PqsE [0.03%]
筛选美国食品药品监督管理局批准的小分子发现抑制铜绿假单胞菌群体感应酶PqsE的抑制剂
Hannah A Jones,Mary J Baxter,Nicolas Zimmermann et al.
Hannah A Jones et al.
Pseudomonas aeruginosa is a notorious pathogen that is a leading cause of hospital-acquired infections, for which there are few treatment options. The quorum sensing (QS) pathway governs many pathogenic behaviors that allow for P. aeruginos...
Bettina Kass,Gereon Poschmann,Fatih Demir et al.
Bettina Kass et al.
Amyloid beta (Aβ) oligomers are thought to play an important role during development and progression of Alzheimer's disease (AD). Previously, we determined the Aβ oligomer concentrations in various AD mouse models and in human brain tissu...
Chemical Biology Drives Membrane-Curvature-Sensing Peptide Probes for Extracellular Vesicle Capture and Applications [0.03%]
化学生物学驱动的膜曲率感应肽探针用于细胞外囊泡捕获及应用
Gaoge Sun,Hang Yin
Gaoge Sun
Extracellular vesicles (EVs) are nanosized lipid bilayer vesicles released by all cells. EVs carry nucleic acids, proteins, lipids, and metabolites in intercellular communication. As potential liquid-biopsy biomarkers and drug-delivery vehi...
Systematic Application of a Cellular Thermal Shift Assay for Inferring Inhibitor Binding Affinity to 17βHSD13 in a Humanized Liver Homogenate [0.03%]
系统应用细胞热移位测定法在人源化肝脏匀浆物中推断对17βHSD13抑制剂的结合亲和力
Sandra Lunnerdal,Fredrik Wågberg,Göran Dahl et al.
Sandra Lunnerdal et al.
Human loss-of-function variants in 17βHSD13 have been associated with reduced risk and progression of metabolic and alcohol-related liver disease. Although in vitro experiments implicate 17βHSD13 in the processing of steroidal and lipogen...