A targeted extracellular approach for recording long-term firing patterns of excitable cells: a practical guide [0.03%]
一种记录可兴奋细胞长期放电模式的靶向细胞外方法：实用指南
Craig S. Nunemaker,R Anthony DeFazio,Suzanne M. Moenter
Craig S. Nunemaker
Excitable cells in many endocrine and neuronal systems display rhythms with periodicities on the order of many minutes. To observe firing patterns that represent the output of these rhythms requires a recording technique that can monitor el...
Evaluation of intra- and interspecific divergence of satellite DNA sequences by nucleotide frequency calculation and pairwise sequence comparison [0.03%]
通过核苷酸频率计算和成对序列比较评估卫星DNA序列的种内和种间差异
Mikio Kato
Mikio Kato
Satellite DNA sequences are known to be highly variable and to have been subjected to concerted evolution that homogenizes member sequences within species. We have analyzed the mode of evolution of satellite DNA sequences in four fishes fro...
Application of a Colorimetric Assay to Identify Putative Ribofuranosylaminobenzene 5'-Phosphate Synthase Genes Expressed with Activity in Escherichia coli [0.03%]
比色法在鉴定大肠杆菌中表达活性的呋喃核糖基氨基苯5'-磷酸合成酶基因中的应用
Matthew E. Bechard,Sonya Chhatwal,Rosemarie E. Garcia et al.
Matthew E. Bechard et al.
Tetrahydromethanopterin (H(4)MPT) is a tetrahydrofolate analog originally discovered in methanogenic archaea, but later found in other archaea and bacteria. The extent to which H(4)MPT occurs among living organisms is unknown. The key enzym...
Use of Site-Specifically Tethered Chemical Nucleases to Study Macromolecular Reactions [0.03%]
利用位点特异性栓系化学核酸酶研究大分子反应
Srabani Mukherjee,Rui Sousa
Srabani Mukherjee
During a complex macromolecular reaction multiple changes in molecular conformation and interactions with ligands may occur. X-ray crystallography may provide only a limited set of snapshots of these changes. Solution methods can augment su...
Methodology for Detecting Trace Amounts of Microchimeric DNA from Peripheral Murine White Blood Cells by Real-Time PCR [0.03%]
实时荧光定量PCR检测小鼠外周血白细胞微量嵌合DNA的方法学
Carol M. Artlett,C Gennaro Dito,Paul J. Christner
Carol M. Artlett
Real-time PCR methodology can successfully quantitate microchimeric cell populations at a concentration of 100 microchimeric cells/100,000 host cells; however, it has not been successful in quantitating DNA from trace numbers of microchimer...
Immunological assays for chemokine detection in in-vitro culture of CNS cells [0.03%]
中枢神经系统细胞体外培养中趋化因子检测的免疫学分析
Supriya D. Mahajan,Stanley A. Schwartz,Madhavan P.N. Nair
Supriya D. Mahajan
Herein we review the various methods currently in use for determining the expression of chemokines by CNS cells in vitro. Chemokine detection assays are used in conjuction with one another to provide a comprehensive, biologically relevant a...
Biological Procedures: An electronic techniques journal serving biochemists and biologists [0.03%]
《生物程序》：一本为生物化学家和生物学家服务的电子技术期刊
Mark Reimer,Dean Sas,Irem Ali
Mark Reimer