A sensitive non-radioactive in situ hybridization method for the detection of chicken IgG gamma-chain mRNA: a technique suitable for detecting of variety of mRNAs in tissue sections [0.03%]
Weiming Zheng,Junichi Izaki,Shuichi Furusawa et al.
Weiming Zheng et al.
We established a sensitive non-radioactive in situ hybridization (ISH) method for the detection of chicken IgG gamma-chain mRNA in paraffin sections. RNA probes were transcribed in vitro from cloned chicken IgG CH1 nucleotide sequences with...
Cytochemical techniques and energy-filtering transmission electron microscopy applied to the study of parasitic protozoa [0.03%]
Marcos A Vannier-Santos,Ulysses Lins
Marcos A Vannier-Santos
The study of parasitic protozoa plays a major role in cell biology, biochemistry and molecular biology. Numerous cytochemical techniques have been developed in order to unequivocally identify the nature of subcellular compartments. Enzyme a...
Semiquantitative RT-PCR analysis to assess the expression levels of multiple transcripts from the same sample [0.03%]
Maria Marone,Simona Mozzetti,Daniela De Ritis et al.
Maria Marone et al.
We describe a semiquantitative RT-PCR protocol optimized in our laboratory to extract RNA from as little as 10,000 cells and to measure the expression levels of several target mRNAs from each sample. This procedure was optimized on the huma...
Acid Glycohydrolases in Rat Spermatocytes, Spermatids and Spermatozoa: Enzyme Activities, Biosynthesis and Immunolocalization [0.03%]
Aida Abou-Haila,Daulat RP Tulsiani
Aida Abou-Haila
Mammalian sperm acrosome contains several glycohydrolases thought to aid in the dispersion and digestion of vestments surrounding the egg. In this study, we have used multiple approaches to examine the origin of acrosome-associated glycohdy...
Measurement of Cardiac Mechanical Function in Isolated Ventricular Myocytes from Rats and Mice by Computerized Video-Based Imaging [0.03%]
Jun Ren,Loren E Wold
Jun Ren
Isolated adult cardiac ventricular myocytes have been a useful model for cardiovascular research for more than 20 years. With the recent advances in cellular physiology and transgenic techniques, direct measurement of isolated ventricular m...
A fluorescence microscopy method for quantifying levels of prostaglandin endoperoxide H synthase-1 and CD-41 in MEG-01 cells [0.03%]
Douglas J Franks,Cameron Mroske,Odette Laneuville
Douglas J Franks
In platelets, PGHS-1-dependant formation of thromboxane A(2) is an important modulator of platelet function and a target for pharmacological inhibition of platelet function by aspirin. Since platelets are a-nucleated cells, we have used the...
Nitin S Baliga
Nitin S Baliga
Gene expression and regulation are mediated by DNA sequences, in most instances, directly upstream to the coding sequences by recruiting transcription factors, regulators, and a RNA polymerase in a spatially defined fashion. Few nucleotides...
Gerhard Schultheiss,Sarah Lan Kocks,Martin Diener
Gerhard Schultheiss
Isolated epithelial cells from intestinal mucosae are a suitable object for the study of the regulation of ion transport in the gut. This regulation possesses a great importance for human and veterinary medicine, as diarrheal diseases, whic...
Investigation of Neuronal Cell Type-Specific Gene Expression of Ca2+/Calmodulin-dependent Protein Kinase II [0.03%]
Kazuko Mima,Hitomi Donai,Takashi Yamauchi
Kazuko Mima
The promoter activity of the rat Ca(2+)/calmodulin-dependent protein kinase II gene was analyzed using the luciferase reporter gene in neuronal and non-neuronal cell lines. Neuronal cell type-specific promoter activity was found in the 5'-f...
Detection of Progeny Immune Responses after Intravenous Administration of DNA Vaccine to Pregnant Mice [0.03%]
Ke-Qin Xin,Shin Sasaki,Yoshitsugu Kojima et al.
Ke-Qin Xin et al.
A number of factors influence the development of tolerance, including the nature, concentration and mode of antigen presentation to the immune system, as well as the age of the host. The studies were conducted to determine whether immunizin...