Raymond L. Yung,Donna Ray,Ru Ran Mo et al.
Raymond L. Yung et al.
Integrin adhesion molecules have important adhesion and signaling functions. They also play a central role in the pathogenesis of many autoimmune diseases. Over the past few years we have described a T cell adoptive transfer model to invest...
A screen to identify drug resistant variants to target-directed anti-cancer agents [0.03%]
一种筛查抗癌药物耐药突变体的系统
Mohammad Azam,Tal Raz,Valentina Nardi et al.
Mohammad Azam et al.
The discovery of oncogenes and signal transduction pathways important for mitogenesis has triggered the development of target-specific small molecule anti-cancer compounds. As exemplified by imatinib (Gleevec), a specific inhibitor of the C...
Development of a new protocol for 2-day generation of mature dendritic cells from human monocytes [0.03%]
一种由人单核细胞生成成熟树突状细胞的新方案的建立及验证
Bianca Obermaier,Marc Dauer,Jan Herten et al.
Bianca Obermaier et al.
We developed a new 2-day protocol for the generation of dendritic cells (DCs) from human monocytes in vitro. First, we demonstrated that 24 hours of culture with GM-CSF and IL-4 are sufficient to generate immature DCs capable of antigen upt...
Swarming populations of Salmonella represent a unique physiological state coupled to multiple mechanisms of antibiotic resistance [0.03%]
沙门氏菌群聚代表一种独特的生理状态并伴有多种抗生素抗性机制
Wook Kim,Michael G. Surette
Wook Kim
Salmonella enterica serovar Typhimurium is capable of swarming over semi-solid surfaces. Although its swarming behavior shares many readily observable similarities with other swarming bacteria, the phenomenon remains somewhat of an enigma i...
Effector cell mediated cytotoxicity measured by intracellular Granzyme B release in HIV infected subjects [0.03%]
HIV感染者细胞毒性效应细胞介导的细胞毒释放颗粒酶B的测定
Supriya D. Mahajan,Ravikumar Aalinkeel,Stanley A. Schwartz et al.
Supriya D. Mahajan et al.
CD8+ cytotoxic T lymphocyte (CTL) activity is currently believed to be one of the key immunologic mechanisms responsible for the prevention or attenuation of HIV-1 infection. The induction of CD8+ T cell activation may also result in the pr...
Use of surface plasmon resonance for the measurement of low affinity binding interactions between HSP72 and measles virus nucleocapsid protein [0.03%]
表面等离子体共振技术在测量HSP72与麻疹病毒核蛋白的低亲和力结合中的应用研究
Xinsheng Zhang,Michael Oglesbee
Xinsheng Zhang
The 72 kDa heat shock protein (HSP72) is a molecular chaperone that binds native protein with low affinity. These interactions can alter function of the substrate, a property known as HSP-mediated activity control. In the present work, BIAc...
Methods designed for the identification and characterization of in vitro and in vivo chromatin assembly mutants in Saccharomyces cerevisiae [0.03%]
用于鉴定和表征酿酒酵母(Saccharomyces cerevisiae)体内外染色质装配突变株的方法
Troy A. A. Harkness,Terra G. Arnason,Charmaine Legrand et al.
Troy A. A. Harkness et al.
Assembly of DNA into chromatin allows for the formation of a barrier that protects naked DNA from protein and chemical agents geared to degrade or metabolize DNA. Chromatin assembly occurs whenever a length of DNA becomes exposed to the cel...
Lara A. Underkoffler,Joelle N. Collins,Jonathan D. Choi et al.
Lara A. Underkoffler et al.
Microsatellite markers are simple sequence repeats within the mammalian genome that can be used for identifying disease loci, mapping genes of interest as well as studying segregation patterns related to meiotic nondisjunction. Different st...
Shotgun Phage Display - Selection for Bacterial Receptins or other Exported Proteins [0.03%]
散弹枪噬菌体展示技术-用于选择细菌受体或其它分泌蛋白的技术
Karin Jacobsson,Anna Rosander,Joakim Bjerketorp et al.
Karin Jacobsson et al.
Shotgun phage display cloning involves construction of libraries from randomly fragmented bacterial chromosomal DNA, cloned genes, or eukaryotic cDNAs, into a phagemid vector. The library obtained consists of phages expressing polypeptides ...
Helder Maiato,Claudio E. Sunkel,William C. Earnshaw
Helder Maiato
Here we describe a detailed methodology to study the function of genes whose products function during mitosis by dsRNA-mediated interference (RNAi) in cultured cells of Drosophila melanogaster. This procedure is particularly useful for the ...