Suppression subtractive hybridization coupled with microarray analysis to examine differential expression of genes in virus infected cells [0.03%]
利用微列阵分析技术研究病毒感染细胞中不同基因的表达状况
Shirin Munir,Sushmita Singh,Kuljeet Kaur et al.
Shirin Munir et al.
High throughput detection of differential expression of genes is an efficient means of identifying genes and pathways that may play a role in biological systems under certain experimental conditions. There exist a variety of approaches that...
Serum-Free Cryopreservation of Five Mammalian Cell Lines in Either a Pelleted or Suspended State [0.03%]
无血清冷冻保存五种哺乳动物细胞系（呈沉淀状或悬浮状）
Joe Corsini,Christy Hacker,Charles Bare
Joe Corsini
Herein we have explored two practical aspects of cryopreserving cultured mammalian cells during routine laboratory maintenance. First, we have examined the possibility of using a serum-free, hence more affordable, cryopreservative. Using fi...
Organotypic cocultures as skin equivalents: A complex and sophisticated in vitro system [0.03%]
类器官型共培养体系——一种复杂的体外皮肤模型系统
Hans-Jürgen Stark,Axel Szabowski,Norbert E. Fusenig et al.
Hans-Jürgen Stark et al.
To assess the role of genes required for skin organogenesis, tissue regeneration and homeostasis, we have established in vitro skin equivalents composed of primary cells or cell lines, respectively. In these organotypic cocultures keratinoc...
Ribosome formation from subunits studied by stopped-flow and Rayleigh light scattering [0.03%]
通过停流和瑞利光散射研究核糖体亚基的形成
Ayman Antoun,Michael Y. Pavlov,Tanel Tenson et al.
Ayman Antoun et al.
Light scattering and standard stopped-flow techniques were used to monitor rapid association of ribosomal subunits during initiation of eubacterial protein synthesis. The effects of the initiation factors IF1, IF2, IF3 and buffer conditions...
Negative Staining and Image Classification - Powerful Tools in Modern Electron Microscopy [0.03%]
负染色和图像分类技术在现代电子显微镜中的强大作用
Melanie Ohi,Ying Li,Yifan Cheng et al.
Melanie Ohi et al.
Vitrification is the state-of-the-art specimen preparation technique for molecular electron microscopy (EM) and therefore negative staining may appear to be an outdated approach. In this paper we illustrate the specific advantages of negati...
Phosphopeptide mapping of proteins ectopically expressed in tissue culture cell lines [0.03%]
组织培养细胞系中异常表达蛋白质的磷酸肽图谱分析
Beth A. Firulli,David M. Virshup,Anthony B. Firulli
Beth A. Firulli
Post-translational modifications such as phosphorylation play a vital role in the regulation of protein function. In our study of the basic Helix-loop-Helix (bHLH) transcription factor HAND1, it was suspected that HAND1 was being phosphoryl...
Oscar Zaragoza,Arturo Casadevall
Oscar Zaragoza
Experimental modulation of capsule size is an important technique for the study of the virulence of the encapsulated pathogen Cryptococcus neoformans. In this paper, we summarize the techniques available for experimental modulation of capsu...
Force and Compliance Measurements on Living Cells Using Atomic Force Microscopy (AFM) [0.03%]
活细胞的原子力显微镜力学性能测量研究
Ewa P. Wojcikiewicz,Xiaohui Zhang,Vincent T. Moy
Ewa P. Wojcikiewicz
We describe the use of atomic force microscopy (AFM) in studies of cell adhesion and cell compliance. Our studies use the interaction between leukocyte function associated antigen-1 (LFA-1)/intercellular adhesion molecule-1 (ICAM-1) as a mo...
Julia W. Pridgeon,Thangiah Geetha,Marie W. Wooten
Julia W. Pridgeon
The UBA domain is a conserved sequence motif among polyubiquitin binding proteins. For the first time, we demonstrate a systematic, high throughput approach to identification of UBA domain-interacting proteins from a proteome-wide perspecti...
Tumor cell-collagen interactions: Identification and semi-quantitative evaluation of selectively-expressed genes by combination of differential display- and multiplex-PCR [0.03%]
肿瘤细胞与胶原的相互作用：结合差异显示和多重PCR鉴定并半定量评价选择性表达基因
Rosalia Sirchia,Valentina Ciacciofera,Claudio Luparello
Rosalia Sirchia
It is widely acknowledged that the presence of extracellular matrix components as substrates can drastically modulate the phenotype and gene expression of cultured cells, including tumor cells. A number of published reports indicated that s...