The Use of Antisense Oligonucleotides in Evaluating Survivin as a Therapeutic Target for Radiation Sensitization in Lung Cancer [0.03%]
反义寡核苷酸在评估Survivin作为放射增敏的肺癌治疗靶点中的应用
Eric T Shinohara,Dennis E Hallahan,Bo Lu
Eric T Shinohara
Elucidating the mechanism of over and under expression of proteins is critical in developing a better understanding of cancer. Multiple techniques are used to examine differential expression of proteins in cells and assess changes in protei...
A method for rapidly screening functionality of actin mutants and tagged actins [0.03%]
一种快速筛选肌动蛋白突变体功能及表达标签肌动蛋白的方法
Heidi Rommelaere,Davy Waterschoot,Katrien Neirynck et al.
Heidi Rommelaere et al.
Recombinant production and biochemical analysis of actin mutants has been hampered by the fact that actin has an absolute requirement for the eukaryotic chaperone CCT to reach its native state. We therefore have developed a method to rapidl...
Screening of Compounds Toxicity against Human Monocytic cell line-THP-1 by Flow Cytometry [0.03%]
流式细胞仪筛选化合物对人单核细胞系THP-1的毒性
Neora Pick,Scott Cameron,Dorit Arad et al.
Neora Pick et al.
The worldwide rapid increase in bacterial resistance to numerous antibiotics requires on-going development of new drugs to enter the market. As the development of new antibiotics is lengthy and costly, early monitoring of compound's toxicit...
Using the cre-lox recombination system to assess functional impairment caused by amino acid substitutions in yeast proteins [0.03%]
利用_cre-lox_重组系统评估酵母蛋白质氨基酸替代造成的功能损伤
Renee L Shirley,M Rachel Richards,Michael R Culbertson
Renee L Shirley
A method was developed to assess the functional significance of a sequence motif in yeast Upf3p, a protein required for nonsense-mediated mRNA decay (NMD). The motif lies at the edge of the Upf3p-Upf2p interaction domain, but at the same ti...
Peptide substrate identification for yeast Hsp40 Ydj1 by screening the phage display library [0.03%]
筛选噬菌体展示肽库鉴定酵母Hsp40 Ydj1底物肽段substrate
Jingzhi Li,Bingdong Sha
Jingzhi Li
We have identified a peptide substrate for molecular chaperone Hsp40 Ydj1 by utilizing the combination of phage display library screening and isothemol titration calirimetry (ITC). The initial peptide substrate screening for Hsp40 Ydj1 has ...
Methods for Analyzing the Role of DNA Methylation and Chromatin Structure in Regulating T Lymphocyte Gene Expression [0.03%]
分析DNA甲基化和染色质结构在调节T淋巴细胞基因表达中的作用的方法
Qianjin Lu,Bruce Richardson
Qianjin Lu
Chromatin structure, determined in part by DNA methylation, is established during differentiation and prevents expression of genes unnecessary for the function of a given cell type. We reported that DNA methylation and chromatin structure c...
Neeraja M. Krishnan,Sameer Z. Raina,David D. Pollock
Neeraja M. Krishnan
Substitution patterns among nucleotides are often assumed to be constant in phylogenetic analyses. Although variation in the average rate of substitution among sites is commonly accounted for, variation in the relative rates of specific typ...
Jessica L. Dunne,Adam P. Goobic,Scott T. Acton et al.
Jessica L. Dunne et al.
Leukocyte endothelial cell interaction is a fundamentally important process in many disease states. Current methods to analyze such interactions include the parallel-plate flow chamber and intravital microscopy. Here, we present an improvem...
A uniform procedure for the purification of CDK7/CycH/MAT1, CDK8/CycC and CDK9/CycT1 [0.03%]
用于纯化CDK7/CycH/MAT1、CDK8/CycC和CDK9/CycT1的统一程序
Reena Pinhero,Peter Liaw,Krassimir Yankulov
Reena Pinhero
We have established a uniform procedure for the expression and purification of the cyclin-dependent kinases CDK7/CycH/MAT1, CDK8/CycC and CDK9/CycT1. We attach a His(6)-tag to one of the subunits of each complex and then co-express it toget...
An in vitro assay to study the recruitment and substrate specificity of chromatin modifying enzymes [0.03%]
一种研究染色质修饰酶募集及底物特异性的体外实验体系
Michiel Vermeulen,Hendrik G. Stunnenberg
Michiel Vermeulen
Post-translational modifications of core histones play an important role in regulating fundamental biological processes such as DNA repair, transcription and replication. In this paper, we describe a novel assay that allows sequential targe...