A CRISPR-del-based pipeline for complete gene knockout in human diploid cells [0.03%]
基于CRISPR-del的人二倍体细胞完整基因敲除体系
Takuma Komori,Shoji Hata,Akira Mabuchi et al.
Takuma Komori et al.
The advance of CRISPR/Cas9 technology has enabled us easily to generate gene knockout cell lines by introducing insertion-deletion mutations (indels) at the target site via the error-prone non-homologous end joining repair system. Frameshif...
Cell cycle-dependent palmitoylation of protocadherin 7 by ZDHHC5 promotes successful cytokinesis [0.03%]
细胞周期依赖性的ZDHHC5介导的原钙粘蛋白7棕榈酰化促进成功的胞质分裂
Nazlı Ezgi Ozkan,Berfu Nur Yigit,Beste Senem Degirmenci et al.
Nazlı Ezgi Ozkan et al.
Cell division requires dramatic reorganization of the cell cortex, which is primarily driven by the actomyosin network. We previously reported that protocadherin 7 (PCDH7) gets enriched at the cell surface during mitosis, which is required ...
Vps9d1 regulates tubular endosome formation through specific activation of Rab22A [0.03%]
Vps9d1通过特异性激活Rab22A调节管状胞内体的形成
Shumpei Nakashima,Takahide Matsui,Mitsunori Fukuda
Shumpei Nakashima
The small GTPase Rab22A is an important regulator of the formation of tubular endosomes, which are one of the types of recycling endosome compartments of the clathrin-independent endocytosis pathway. In order to regulate tubular endosome fo...
Peiwei Liu,Ying Liu,Jun Zhou
Peiwei Liu
Cilia are surface-exposed organelles that provide motility and sensory functions for cells, and it is widely believed that mechanosensation can be mediated through cilia. Polycystin-1 and -2 (PC-1 and PC-2, respectively) are transmembrane p...
RNA localization to the mitotic spindle is essential for early development and is regulated by kinesin-1 and dynein [0.03%]
RNA定位到有丝分裂纺锤体对于早期发育至关重要，并且受动力蛋白和驱动蛋白-1的调控
Carolyn M Remsburg,Kalin D Konrad,Jia L Song
Carolyn M Remsburg
Mitosis is a fundamental and highly regulated process that acts to faithfully segregate chromosomes into two identical daughter cells. Localization of gene transcripts involved in mitosis to the mitotic spindle might be an evolutionarily co...
Rac1 and Rac3 GTPases and TPC2 are required for axonal outgrowth and migration of cortical interneurons [0.03%]
Rac1和Rac3鸟苷三磷酸酶及TPC2是皮质中间神经元轴突生长和迁移所必需的
Zouzana Kounoupa,Simona Tivodar,Kostas Theodorakis et al.
Zouzana Kounoupa et al.
Rho GTPases, among them Rac1 and Rac3, are major transducers of extracellular signals and are involved in multiple cellular processes. In cortical interneurons, the neurons that control the balance between excitation and inhibition of corti...
LRRK1 functions as a scaffold for PTP1B-mediated EGFR sorting into ILVs at the ER-endosome contact site [0.03%]
LRRK1作为PTP1B介导的EGFR分类进入ER-内体连接位点ILV的支架功能
Hiroshi Hanafusa,Keitaro Fujita,Misa Kamio et al.
Hiroshi Hanafusa et al.
Proper control of epidermal growth factor receptor (EGFR) signaling is important for maintaining cellular homeostasis. Given that EGFR signaling occurs at the plasma membrane and endosomes following internalization, endosomal trafficking of...
An essential endoplasmic reticulum-resident N-acetyltransferase ortholog in Plasmodium falciparum [0.03%]
恶性疟原虫必需的内质网定植型N-乙酰转移酶同源蛋白
Alexander J Polino,Muhammad M Hasan,Katherine Floyd et al.
Alexander J Polino et al.
N-terminal acetylation is a common eukaryotic protein modification that involves the addition of an acetyl group to the N-terminus of a polypeptide. This modification is largely performed by cytosolic N-terminal acetyltransferases (NATs). M...
Myo19 tethers mitochondria to endoplasmic reticulum-associated actin to promote mitochondrial fission [0.03%]
肌球蛋白 Myo19通过将线粒体与内质网相连的肌动蛋白连接起来促进线粒体分裂
Stephen M Coscia,Cameron P Thompson,Qing Tang et al.
Stephen M Coscia et al.
Mitochondrial homeostasis requires a dynamic balance of fission and fusion. The actin cytoskeleton promotes fission, and we found that the mitochondrially localized myosin, myosin 19 (Myo19), is integral to this process. Myo19 knockdown ind...
Structure and function of distal and subdistal appendages of the mother centriole [0.03%]
母中心体远端和亚远端附属结构的结构与功能
Dandan Ma,Fulin Wang,Junlin Teng et al.
Dandan Ma et al.
Centrosomes are composed of centrioles surrounded by pericentriolar material. The two centrioles in G1 phase are distinguished by the localization of their appendages in the distal and subdistal regions; the centriole possessing both types ...