SILAC-based discovery of a broad range of proteins that can be posttranslationally glutamylated or glycylated [0.03%]
基于 SILAC 的发现可被进行蛋白酶处理的谷氨酰化或甘氨酰化的广泛蛋白质种类
Sucheta Dey,Sanjana Mullick,Veronique Henriot et al.
Sucheta Dey et al.
The posttranslational modifications (PTMs) glutamylation and glycylation are primarily associated with tubulins and microtubule regulation, while their broader functional scope is largely underexplored. It is known that both PTMs also occur...
A screening pipeline to characterize stress-induced enzymes uncovers a cellular function for the poorly characterized alcohol dehydrogenase Bdh2 [0.03%]
一种鉴定压力诱导产生的酶的筛选途径揭秘了作用尚不清楚的乙醇脱氢酶Bdh2的细胞功能
Dunya Edilbi,Rosario Valenti,Benjamin Dubreuil et al.
Dunya Edilbi et al.
Cells possess intricate metabolic networks comprised of hundreds of enzymes. Despite extensive research, many of these enzymes remain uncharacterized. Identifying the function of these enzymes is crucial for advancing our understanding of c...
A RhoGEF activates EhRacM to selectively promote motility without affecting macropinocytosis in Entamoeba histolytica [0.03%]
EhSGST5通过活化EhRacM促进运动性但不影响巨胞饮作用
Misato Shimoyama,Kumiko Nakada-Tsukui,Tomoyoshi Nozaki
Misato Shimoyama
Rho guanine nucleotide-exchange factors (RhoGEFs) activate Rho/Rac small GTPases, orchestrating cellular processes, such as actin remodeling, endocytosis, and migration. In the protozoan parasite Entamoeba histolytica, the causative agent o...
Understanding the molecular components of mechanically evoked currents in the Merkel cell line MCC13 [0.03%]
了解机械刺激诱发的麦克尔细胞系MCC13电流的分子组成
Zijing Zhou,Sonia Paz-López,Lennart Kuck et al.
Zijing Zhou et al.
Merkel cells are epithelial cells involved in the discrimination of light touch. Situated in the skin, these specialised cells decode mechanical cues through the mechanosensitive ion channel PIEZO2. Merkel cell carcinoma lines have been wid...
Douglas R Green
Douglas R Green
The fact that cells die during development, metamorphosis and tissue homeostasis was recognized well over a century ago. However, aside from noting and classifying such cell death events, research into the mechanisms of regulated cell death...
Glucocorticoids regulate small extracellular vesicle release via activation of nSMase2 [0.03%]
糖皮质激素通过激活nSMase2调节微小胞外囊泡的释放
Mia R Burke,Clarissa L Waites
Mia R Burke
Glucocorticoid (GC) steroid hormones mediate the stress response, which requires dynamic communication between brain regions to enable an organism to react to and overcome perceived stressors. While many effects of GCs on the brain have bee...
Chromogranin B plays an essential role in post-endoplasmic reticulum sorting of dense-core vesicle cargo [0.03%]
染色粒素B在密集核心囊泡载体出内质网分拣过程中的重要作用
Chandramouli Mukherjee,Palki Chauksey,Mohima Mukherjee et al.
Chandramouli Mukherjee et al.
Chromogranin B (ChgB) is a major dense-core vesicle (DCV) matrix protein whose mechanistic role in DCV function remains ambiguous. To address this, we used CRISPR-Cas9-mediated genetic ablation, combined with quantitative microscopy and bio...
Divergent protein kinase A contributes to the regulation of flagellar waveforms in Leishmania mexicana [0.03%]
分歧蛋白激酶A参与利什曼原虫调节鞭毛波形的调控
Sophia Fochler,Benjamin J Walker,Richard J Wheeler et al.
Sophia Fochler et al.
The second messenger cyclic adenosine monophosphate (cAMP) and protein kinase A (PKA) have been implicated in the regulation of flagellar motility in diverse eukaryotes, yet the specific contributions of PKA isoforms remain unclear. Moreove...
Pro- and anti-viral functions of lipid droplets in infection at a glance [0.03%]
脂滴在感染中的促病毒和抗病毒功能要点 взглядыв свѣтлуньки På en tankestrekking
Kim Chi Thi Vu,Laura Bierau,Eva Herker
Kim Chi Thi Vu
Lipid droplets (LDs) were long regarded as passive storage organelles for neutral lipids as this is the most efficient way to store energy. Recently, however, they have emerged as highly dynamic and heterogenous organelles that act as centr...
François Lapraz,Céline Boutres,Baptiste Monterroso et al.
François Lapraz et al.
Optical microscopes are essential tools for magnifying and analysing a wide range of samples and are generally expected to faithfully preserve the original object in all its dimensions. However, depending on the optical design and software ...