Chromogranin B plays an essential role in post-endoplasmic reticulum sorting of dense-core vesicle cargo [0.03%]
染色粒素B在密集核心囊泡载体出内质网分拣过程中的重要作用
Chandramouli Mukherjee,Palki Chauksey,Mohima Mukherjee et al.
Chandramouli Mukherjee et al.
Chromogranin B (ChgB) is a major dense-core vesicle (DCV) matrix protein whose mechanistic role in DCV function remains ambiguous. To address this, we used CRISPR-Cas9-mediated genetic ablation, combined with quantitative microscopy and bio...
Divergent protein kinase A contributes to the regulation of flagellar waveforms in Leishmania mexicana [0.03%]
分歧蛋白激酶A参与利什曼原虫调节鞭毛波形的调控
Sophia Fochler,Benjamin J Walker,Richard J Wheeler et al.
Sophia Fochler et al.
The second messenger cyclic adenosine monophosphate (cAMP) and protein kinase A (PKA) have been implicated in the regulation of flagellar motility in diverse eukaryotes, yet the specific contributions of PKA isoforms remain unclear. Moreove...
Pro- and anti-viral functions of lipid droplets in infection at a glance [0.03%]
脂滴在感染中的促病毒和抗病毒功能要点 взглядыв свѣтлуньки På en tankestrekking
Kim Chi Thi Vu,Laura Bierau,Eva Herker
Kim Chi Thi Vu
Lipid droplets (LDs) were long regarded as passive storage organelles for neutral lipids as this is the most efficient way to store energy. Recently, however, they have emerged as highly dynamic and heterogenous organelles that act as centr...
François Lapraz,Céline Boutres,Baptiste Monterroso et al.
François Lapraz et al.
Optical microscopes are essential tools for magnifying and analysing a wide range of samples and are generally expected to faithfully preserve the original object in all its dimensions. However, depending on the optical design and software ...
DySTrack: a modular smart microscopy tool for live tracking of dynamic samples on modern commercial microscopes [0.03%]
DySTrack：一种模块化的智能显微镜工具可对现代商业显微镜上的动态样品进行实时跟踪
Zimeng Wu,Octavian Voiculescu,Alessandro Mongera et al.
Zimeng Wu et al.
Advances in microscopy and bioimage analysis are enabling unprecedented quantitative observation of dynamic biological systems. Smart microscopy closes the loop by feeding back image-derived information to control image acquisition on the f...
Complementary volume electron microscopy-based approaches reveal ultrastructural changes in germline intercellular bridges [0.03%]
互补的体电子显微镜方法揭示了生殖细胞间桥连接的超微结构变化
Irina Kolotuev,Sophie Khazanov,Abigayle Williams et al.
Irina Kolotuev et al.
The Drosophila melanogaster egg chamber is a powerful model system to study germline intercellular bridges, or ring canals, which connect the developing oocyte to supporting nurse cells. Despite their importance, it is technically difficult...
Temperature-specific regulation of the NDR kinase Orb6 by the MAPK Sty1 to promote heat stress resilience [0.03%]
MAPK Sty1通过温度特异性调节NDR激酶Orb6来增强热胁迫耐受性
Laura P Doyle,Robert N Tams,Chuan Chen et al.
Laura P Doyle et al.
The cellular response to environmental fluctuations, such as increased temperature, is crucial in promoting cell survival and plays an increasingly recognized role in cancer biology. Important cellular functions altered by heat stress are c...
Farnesylated prelamin A induces fibroblast polarity defects in premature aging disorders by inhibiting nesprin-2-SUN2 LINC complex function [0.03%]
焦磷酸法尼基化前体突变核纤层蛋白通过抑制nesprin-2-SUN2大型胞质衔接复合物的功能引起早衰症成纤维细胞极性缺陷
Chonkit Lio,Yuexia Wang,Paige C Wilson et al.
Chonkit Lio et al.
Disrupted processing of prelamin A causes Hutchinson-Gilford progeria syndrome (HGPS) and related premature aging disorders. The farnesylated prelamin A variant produced in HGPS, termed progerin, alters actin-nuclear interactions mediated b...
Regulation of Citron kinase by CDK1 and Aurora B regulates midbody formation and stability [0.03%]
CDK1和Aurora B对Citron激酶的调节控制中体的形成与稳定性
Luisa Capalbo,Ella F J Halcrow,Zuni I Bassi et al.
Luisa Capalbo et al.
Many cell division events are regulated by protein phosphorylation, which can result from cross-regulatory mechanisms among mitotic kinases and phosphatases that have yet to be fully elucidated. Here, we report the characterization of a nov...
Molecular features of myosin F adapted for driving actin flows in Toxoplasma gondii [0.03%]
托拉川吸虫肌球蛋白F适应驱动肌动蛋白流的分子特征
Thomas E Sladewski,Aoife T Heaslip
Thomas E Sladewski
Toxoplasma gondii is a single-celled apicomplexan parasite that relies on a highly polarized endomembrane system for its invasion of, and survival within, host cells. Recent advances in imaging technologies have revealed that vesicle transp...