Mitochondrial protein aggregates recruit key chaperones and are sequestered in a fission/fusion-dependent process [0.03%]
线粒体蛋白质聚集物通过依赖于分裂和融合的过程募集关键分子伴侣并被分选出来
Laura Ruland,Marc Sylvester,Lydia Maus et al.
Laura Ruland et al.
The potential proteotoxicity of mitochondrial aggregates in yeast cells is reduced by a sequestration of affected polypeptides into a mitochondrial protein quality control compartment (IMiQ). Based on the expression of an aggregation-prone ...
Shun Kato,Naoki Okada,Toshiki Ohata et al.
Shun Kato et al.
Acyl-CoA synthetases (ACSLs) are a family of enzymes that convert intracellular fatty acids into acyl-CoA. A previous study has demonstrated that the yeast ACSL Faa1 (a homolog of mammalian ACSL4) is involved in autophagosome membrane elong...
Cell volume regulates terminal differentiation of cultured human epidermal keratinocytes [0.03%]
细胞体积调节培养的人表皮角质形成细胞的分化成熟过程
Sebastiaan Zijl,Toru Hiratsuka,Atefeh Mobasseri et al.
Sebastiaan Zijl et al.
To gain insights into the human epidermal stem cell niche we have previously identified micron-scale topographical substrates that regulate differentiation of spread keratinocytes. On one substrate (S1), cells interact with circular topogra...
Arsenic binds to nuclear transport factors and disrupts nucleocytoplasmic transport [0.03%]
砷与核转运因子结合并干扰核质运输
Emma Lorentzon,Jongmin Lee,Jakub Masaryk et al.
Emma Lorentzon et al.
Human exposure to arsenicals is associated with devastating diseases such as cancer and neurodegeneration. At the same time, arsenic-based drugs are used as therapeutic agents. The ability of arsenic to directly bind to proteins is correlat...
Anna Roza Dimogkioka,Anni Elias,Doron Rapaport
Anna Roza Dimogkioka
The outer mitochondrial membrane (OMM) hosts a variety of proteins such as import machineries, enzymes, fission/fusion factors, and pore proteins. In Saccharomyces cerevisiae, the MIM complex, consisting of Mim1 and Mim2, mediates the inser...
Creation and validation of a proteome-wide yeast library for protein detection and analysis [0.03%]
蛋白质检测和分析的酵母蛋白组文库的创建及验证
Din Baruch,Ioannis Tsirkas,Ehud Sass et al.
Din Baruch et al.
A significant challenge in cell biology is to uncover the function of uncharacterized proteins. Surprisingly a quarter of the proteome is still poorly understood even in the most well studied model organisms. Systematic methodologies, inclu...
Sub-ciliary localization of CEP290 and effects of its loss in mouse photoreceptors during development [0.03%]
CEP290在睫状沟的定位及其在小鼠视杆细胞发育过程中丢失的影响
Abigail R Moye,Michael A Robichaux,Melina A Agosto et al.
Abigail R Moye et al.
The most common genetic cause of the childhood blinding disease Leber congenital amaurosis is mutation of the ciliopathy gene CEP290. Despite extensive study, the photoreceptor-specific roles of CEP290 remain unclear. Using advanced microsc...
APEX2-based quantitative proteomics of LAT and CD3σ interactomes in living human Jurkat T cells unveils new interactors [0.03%]
基于APEX2的定量蛋白质组学揭示活体人类Jurkat T细胞中LAT和CD3σ互作蛋白的新成员
Juan-José Saez,Michaël Richard,Vivien Caillens et al.
Juan-José Saez et al.
TCR stimulation induces a signaling cascade starting by the phosphorylation of immunoreceptor tyrosine-based activation motif (ITAMs) present in the TCR-CD3 complex. This is followed by the phosphorylation of proteins including LAT, which o...
Cell cycle- and dose-dependent effects on mitochondrial DNA copy number variation following irradiation [0.03%]
辐照后细胞周期及剂量依赖性对线粒体DNA拷贝数变异的影响
Ryosuke Seino,Kai Nishikubo,Hisanori Fukunaga
Ryosuke Seino
Cell survival after irradiation depends on the cell cycle at the time of exposure. This has been thought to be due to cell cycle-dependent nuclear DNA damage repair mechanisms. Here we show the relationships between the exposed dose, the ce...
Front-biased activation of Ras-Rab5-Rac1 loop coordinates collective cell migration [0.03%]
前端偏向激活的Ras-Rab5-Rac1环路协调集体细胞迁移
Yuya Jikko,Eriko Deguchi,Kimiya Matsuda et al.
Yuya Jikko et al.
Collective cell migration is coordinated by the front-to-rear intercellular propagation of EGFR-Ras-ERK pathway activation. However, the molecular mechanisms integrating front-to-rear information into this intercellular signaling cascade, p...