Hideaki Takano,Tatsuya Nishiyama,Sho-ichi Amano et al.
Hideaki Takano et al.
Streptomyces and related bacteria produce a wide variety of secondary metabolites. Of these, many compounds have industrial applications, but the question of why this group of microorganism produces such various kinds of biologically active...
Effects of heterologous expression of phosphoenolpyruvate carboxykinase and phosphoenolpyruvate carboxylase on organic acid production in Aspergillus carbonarius [0.03%]
异源表达磷酸烯醇式丙酮酸羧基酶和磷酸烯醇式丙酮酸羧激酶对碳球茎丝状菌有机酸产生的影响
Lei Yang,Mette Lübeck,Peter S Lübeck
Lei Yang
Aspergillus carbonarius has a potential as a cell factory for production of various organic acids. In this study, the organic acid profile of A. carbonarius was investigated under different cultivation conditions. Moreover, two heterologous...
EasyClone 2.0: expanded toolkit of integrative vectors for stable gene expression in industrial Saccharomyces cerevisiae strains [0.03%]
简便克隆2.0版：工业毕赤酵母菌株稳定基因表达的整合型载体扩展工具包
Vratislav Stovicek,Gheorghe M Borja,Jochen Forster et al.
Vratislav Stovicek et al.
Saccharomyces cerevisiae is one of the key cell factories for production of chemicals and active pharmaceuticals. For large-scale fermentations, particularly in biorefinery applications, it is desirable to use stress-tolerant industrial str...
Genetic manipulation of secondary metabolite biosynthesis for improved production in Streptomyces and other actinomycetes [0.03%]
放线菌中次级代谢产物生物合成的遗传调控及改良生产
Richard H Baltz
Richard H Baltz
Actinomycetes continue to be important sources for the discovery of secondary metabolites for applications in human medicine, animal health, and crop protection. With the maturation of actinomycete genome mining as a robust approach to iden...
Development of engineered Escherichia coli whole-cell biocatalysts for high-level conversion of L-lysine into cadaverine [0.03%]
高效转化赖氨酸制备 cadaverine 工程细胞催化剂的开发
Young Hoon Oh,Kyoung-Hee Kang,Mi Jeong Kwon et al.
Young Hoon Oh et al.
A whole-cell biocatalytic system for the production of cadaverine from L-lysine has been developed. Among the investigated lysine decarboxylases from different microorganisms, Escherichia coli LdcC showed the best performance on cadaverine ...
Discovery of novel drug targets and their functions using phenotypic screening of natural products [0.03%]
利用天然产物的表型筛选发现新药靶点及其功能
Junghwa Chang,Ho Jeong Kwon
Junghwa Chang
Natural products are valuable resources that provide a variety of bioactive compounds and natural pharmacophores in modern drug discovery. Discovery of biologically active natural products and unraveling their target proteins to understand ...
Informatic search strategies to discover analogues and variants of natural product archetypes [0.03%]
用于发现天然产物原型类似物和变异的informatics搜索策略
Chad W Johnston,Alex D Connaty,Michael A Skinnider et al.
Chad W Johnston et al.
Natural products are a crucial source of antimicrobial agents, but reliance on low-resolution bioactivity-guided approaches has led to diminishing interest in discovery programmes. Here, we demonstrate that two in-house automated informatic...
Production of C4 and C5 branched-chain alcohols by engineered Escherichia. coli [0.03%]
工程化大肠杆菌生产C4和C5支链醇
Xiaoyan Chen,Jingliang Xu,Liu Yang et al.
Xiaoyan Chen et al.
Higher alcohols, longer chain alcohols, contain more than 3 carbon atoms, showed close energy advantages as gasoline, and were considered as the next generation substitution for chemical fuels. Higher alcohol biosynthesis by native microorg...
Structural and functional analysis of a low-temperature-active alkaline esterase from South China Sea marine sediment microbial metagenomic library [0.03%]
来自中国南海海洋沉积物微生物元基因组文库的低温活性碱性酯酶的结构和功能分析
Yongfei Hu,Yinghui Liu,Jing Li et al.
Yongfei Hu et al.
A low-temperature-active alkaline esterase, Est12, from a marine sediment metagenomic fosmid library was identified. Est12 prefers short- and middle-chain p-nitrophenol esters as substrate with optimum temperature and pH value of 50 °C and...
The biosynthetic pathway of FK506 and its engineering: from past achievements to future prospects [0.03%]
FK506的生物合成途径及其工程化研究：从过去到未来
Yeon Hee Ban,Sung Ryeol Park,Yeo Joon Yoon
Yeon Hee Ban
FK506, a 23-membered macrolide produced by several Streptomyces species, is an immunosuppressant widely used to prevent the rejection of transplanted organs. In addition, FK506 and its analogs possess numerous promising therapeutic potentia...