Integrating mass spectrometry and genomics for cyanobacterial metabolite discovery [0.03%]
整合质谱和基因组学进行蓝藻代谢物发现
Nathan A Moss,Matthew J Bertin,Karin Kleigrewe et al.
Nathan A Moss et al.
Filamentous marine cyanobacteria produce bioactive natural products with both potential therapeutic value and capacity to be harmful to human health. Genome sequencing has revealed that cyanobacteria have the capacity to produce many more s...
Degradation of phenolic compounds by the lignocellulose deconstructing thermoacidophilic bacterium Alicyclobacillus Acidocaldarius [0.03%]
枯草芽孢杆菌Alicyclobacillus acidocaldarius通过解聚木质纤维素使菲尼酮化合物退化
John E Aston,William A Apel,Brady D Lee et al.
John E Aston et al.
Alicyclobacillus acidocaldarius, a thermoacidophilic bacterium, has a repertoire of thermo- and acid-stable enzymes that deconstruct lignocellulosic compounds. The work presented here describes the ability of A. acidocaldarius to reduce the...
C R Strachan,J Davies
C R Strachan
The role of secondary metabolites in effecting and modulating reactions during early biochemical evolution has been largely unappreciated. It is possible that low molecular weight effectors were gradually replaced by polypeptides as polymer...
Harnessing natural product assembly lines: structure, promiscuity, and engineering [0.03%]
利用天然产物合成途径：结构、多功能性及工程改造
Christopher C Ladner,Gavin J Williams
Christopher C Ladner
Many therapeutically relevant natural products are biosynthesized by the action of giant mega-enzyme assembly lines. By leveraging the specificity, promiscuity, and modularity of assembly lines, a variety of strategies has been developed th...
Controlling the transcription levels of argGH redistributed L-arginine metabolic flux in N-acetylglutamate kinase and ArgR-deregulated Corynebacterium crenatum [0.03%]
调控N-乙酰谷氨酸激酶和ArgR去抑制棒状杆菌argGH重分布L-精氨酸代谢流量的转录水平
Qinqin Zhao,Yuchang Luo,Wenfang Dou et al.
Qinqin Zhao et al.
Corynebacterium crenatum SYPA5-5, an L-arginine high-producer obtained through multiple mutation-screening steps, had been deregulated by the repression of ArgR that inhibits L-arginine biosynthesis at genetic level. Further study indicated...
Sonia I Maffioli,João C S Cruz,Paolo Monciardini et al.
Sonia I Maffioli et al.
Natural products represent a major source of approved drugs and still play an important role in supplying chemical diversity. Consistently, 2014 has seen new, natural product-derived antibiotics approved for human use by the US Food and Dru...
Biosynthesis of mercapturic acid derivative of the labdane-type diterpene, cyslabdan that potentiates imipenem activity against methicillin-resistant Staphylococcus aureus: cyslabdan is generated by mycothiol-mediated xenobiotic detoxification [0.03%]
半萜二萜烃类衍生物cyslabdan的生物合成：通过麦考素介导的外来物质解毒增强亚胺培南对耐甲氧西林金黄色葡萄球菌的作用：cyslabdan产生机制研究
Haruo Ikeda,Kazuo Shin-Ya,Tohru Nagamitsu et al.
Haruo Ikeda et al.
Genome mining of cyslabdan-producing Streptomyces cyslabdanicus K04-0144 revealed that a set of four genes, cldA, cldB, cldC, and cldD (the cld cluster), which formed a single transcriptional unit, were involved in the biosynthesis of cysla...
Lei Sun,Fan Yang,Hongbing Sun et al.
Lei Sun et al.
1,2,4-Butanetriol (BT) is an important non-natural chemical with a variety of industrial applications. Identifying the bottlenecks for BT biosynthesis is expected to contribute to improving the efficiency of this process. In this work, we f...
Quantitative relationship between the mRNA secondary structure of translational initiation region and the expression level of heterologous protein in Escherichia coli [0.03%]
异源蛋白质在大肠杆菌中的表达量与转录起始区mRNA二级结构的量化关系研究
Jun Yin,Lichen Bao,Hong Tian et al.
Jun Yin et al.
Translational efficiency in Escherichia coli is strongly influenced by mRNA secondary structure of translational initiation region (TIR). We have previously reported that the expression of heterologous protein is directly related to the min...
Studies on properties of the xylan‑binding domain and linker sequence of xylanase XynG1‑1 from Paenibacillus campinasensis G1‑1 [0.03%]
来自Paenibacillus campinasensis G1-1的木聚糖酶XynG1-1的木聚糖结合域及其连接肽的研究
Yihan Liu,Lin Huang,Weiguo Li et al.
Yihan Liu et al.
Xylanase XynG1-1 from Paenibacillus campinasensis G1-1 consists of a catalytic domain (CD), a family 6_36 carbohydrate-binding module which is a xylan-binding domain (XBD), and a linker sequence (LS)between them. The structure of XynG1-3 fr...