A xylanase from Streptomyces sp. FA1: heterologous expression, characterization, and its application in Chinese steamed bread [0.03%]
来源于链霉菌FA1的木聚糖酶的异源表达、酶学性质及其在馒头加工中的应用研究
Yang Xu,Jing Wu,Kaixuan Zheng et al.
Yang Xu et al.
Xylanases (EC 3.2.1.8) are hydrolytic enzymes that have found widespread application in the food, feed, and paper-pulp industries. Streptomyces sp. FA1 xynA was expressed as a secreted protein in Pichia pastoris, and the xylanase was applie...
A new approach for improving epothilone B yield in Sorangium cellulosum by the introduction of vgb epoF genes [0.03%]
通过引入vgb epoF基因提高Sorangium cellulosum中环氧菌素B产量的新方法
Wei Ye,Weimin Zhang,Yuchan Chen et al.
Wei Ye et al.
Epothilone B has drawn great attention due to its much stronger anticancer activity and weaker side effects compared with taxol. The relative low yield of epothilone B limited its application. In this study, we report the successful introdu...
Production of biorenewable styrene: utilization of biomass-derived sugars and insights into toxicity [0.03%]
生物基苯乙烯的生产：生物质衍生物和毒性的利用和见解
Jieni Lian,Rebekah McKenna,Marjorie R Rover et al.
Jieni Lian et al.
Fermentative production of styrene from glucose has been previously demonstrated in Escherichia coli. Here, we demonstrate the production of styrene from the sugars derived from lignocellulosic biomass depolymerized by fast pyrolysis. A pre...
Optimization of growth medium for Sporosarcina pasteurii in bio-based cement pastes to mitigate delay in hydration kinetics [0.03%]
用于生物基水泥浆以减轻水化动力学延迟的Sporosarcina pasteurii生长培养基的优化
Sarah L Williams,Mary Jo Kirisits,Raissa Douglas Ferron
Sarah L Williams
Microbial-induced calcium carbonate precipitation has been identified as a novel method to improve durability and remediate cracks in concrete. One way to introduce microorganisms to concrete is by replacing the mixing water with a bacteria...
A chimeric α-amylase engineered from Bacillus acidicola and Geobacillus thermoleovorans with improved thermostability and catalytic efficiency [0.03%]
来自Bacillus acidicola和Geobacillus thermoleovorans的嵌合α-淀粉酶的工程设计：提高其热稳定性和催化效率
Deepak Parashar,T Satyanarayana
Deepak Parashar
The α-amylase (Ba-amy) of Bacillus acidicola was fused with DNA fragments encoding partial N- and C-terminal region of thermostable α-amylase gene of Geobacillus thermoleovorans (Gt-amy). The chimeric enzyme (Ba-Gt-amy) expressed in Esche...
Influence of methanol/sorbitol co-feeding rate on pAOX1 induction in a Pichia pastoris Mut+ strain in bioreactor with limited oxygen transfer rate [0.03%]
甲醇/山梨醇混养速率对氧传递速率受限条件下毕赤酵母Mut+菌株pAOX1启动子诱导表达的影响研究
F Carly,H Niu,F Delvigne et al.
F Carly et al.
High Pichia pastoris biomass density could be obtained using high co-feeding rate of methanol and sorbitol in a fed-batch or continuous culture, while further higher feeding rate finally leads to oxygen limitation in bioreactor. In the lite...
Characterization of representative rpoB gene mutations leading to a significant change in toyocamycin production of Streptomyces diastatochromogenes 1628 [0.03%]
导致放线菌链丝菌1628号strain toyocamycin产量显著变化的rpoB基因代表型突变体的特征研究
Zheng Ma,Shuai Luo,Xianhao Xu et al.
Zheng Ma et al.
Modification of enzymes involved in transcription- or translation-processes is an interesting way to increase secondary metabolite production in Streptomycetes. However, application of such methods has not been widely described for strains ...
Proteomics analysis of global regulatory cascades involved in clavulanic acid production and morphological development in Streptomyces clavuligerus [0.03%]
头孢菌素酸产生及链霉菌形态发生的全局调控级联的蛋白质组分析
Nicole L Ferguson,Lourdes Peña-Castillo,Marcus A Moore et al.
Nicole L Ferguson et al.
The genus Streptomyces comprises bacteria that undergo a complex developmental life cycle and produce many metabolites of importance to industry and medicine. Streptomyces clavuligerus produces the β-lactamase inhibitor clavulanic acid, wh...
The yajC gene from Lactobacillus buchneri and Escherichia coli and its role in ethanol tolerance [0.03%]
芽孢乳杆菌和大肠杆菌中的yajC基因及其在耐乙醇性中的作用
Siqing Liu,Chris Skory,Nasib Qureshi et al.
Siqing Liu et al.
The yajC gene (Lbuc_0921) from Lactobacillus buchneri NRRL B-30929 was identified from previous proteomics analyses in response to ethanol treatment. The YajC protein expression was increased by 15-fold in response to 10 % ethanol vs 0 % et...
High-throughput screening of high Monascus pigment-producing strain based on digital image processing [0.03%]
基于数字图像处理的红曲色素高产菌株高效筛选技术
Meng-lei Xia,Lan Wang,Zhi-xia Yang et al.
Meng-lei Xia et al.
This work proposed a new method which applied image processing and support vector machine (SVM) for screening of mold strains. Taking Monascus as example, morphological characteristics of Monascus colony were quantified by image processing....