The biosynthetic gene cluster landscape of the oral microbiome across health and dental caries [0.03%]
口腔微生物组在健康和龋齿状况下的生物合成基因簇特征分布景观分析研究
McKenna Loop Yao,Peijun Lin,Kailey Hua et al.
McKenna Loop Yao et al.
Specialized metabolites encoded by biosynthetic gene clusters (BGCs) in the oral microbiome remain largely unexplored in the context of oral health and disease. Previous genome-centric surveys have identified hundreds of uncharacterized BGC...
Statistical approach for media optimization of fHbp-PorA chimeric protein expression in Escherichia coli; Development of a promising Meningococcal B vaccine [0.03%]
统计方法优化脑膜炎球菌B型结合疫苗候选抗原fHbp-PorA嵌合蛋白在大肠杆菌中的表达
Annamraju Aswini,Annamraju D Sarma,Sambhaji S Pisal et al.
Annamraju Aswini et al.
Successful design of an industrial biological product such as a vaccine requires an efficient, robust and reproducible process. In this study, we investigated conditions for process optimization of a promising recombinant fHbp-PorA chimeric...
Complete Diploid Genome of the Type Strain Yarrowia lipolytica YB-423 (ATCC 18942™) [0.03%]
解脂耶罗酵母标准菌株YB-423（ATCC 18942TM）的全二倍体基因组
James E Crill nd,Scott V Nguyen,Corina Tabron et al.
James E Crill nd et al.
Here, we present the first complete, fully phased diploid genome of type strain Yarrowia lipolytica YB-423 (=ATCC 18942™), constructed using a combination of Oxford Nanopore long-read and Illumina short-read sequencing. Y. lipolytica is an...
Exploration of Yeast Biodiversity from Thai Flowers and Optimization of Carotenoid Production by a Promising Isolate [0.03%]
来自泰国花卉的酵母菌多样性的研究及一类极具潜力的酵母菌色素生产的优化
Pirapan Polburee,Thippawan Kodpan,Krittawan Tondee et al.
Pirapan Polburee et al.
Microbial synthesis of carotenoids has garnered significant attention as an eco-friendly alternative to conventional synthetic methods and its facile extraction for impressive yield. This study delves into the efficacy of carotenoid product...
Synergistic strategies for high production of Geobacillus stearothermophilus α-amylase in Bacillus subtilis [0.03%]
地衣芽孢杆菌α-淀粉酶在枯草芽孢杆菌中高效表达的协同策略研究
Deming Rao,Changhui Wang,Xiaolin Li et al.
Deming Rao et al.
Although the α-amylase (AmyS) from Geobacillus stearothermophilus exhibits high thermostability, its low enzymatic activity (44.36 ± 2.02 U/mL) severely hinders industrial applications. Given its strong protein secretion capability and GR...
Protoplast fusion as a strategy to increase ploidy in Rhodotorula toruloides for strain development [0.03%]
用于雷氏红球盖菌菌株开发的增加染色体倍性的原生质体融合方法
Daiane Dias Lopes,Bruce S Dien,Ronald E Hector et al.
Daiane Dias Lopes et al.
Rhodotorula toruloides is a red oleaginous yeast with growing commercial interest because of its hardiness and exceptional lipid production capacity. Because it is a basidiomycete yeast with a complex life cycle, many of the classical breed...
Production of glycolic acid through whole-cell bioconversion from PET monomer ethylene glycol using engineered Corynebacterium glutamicum [0.03%]
采用工程谷氨酸棒杆菌以PET单体乙二醇为底物胞内转化合成甘醇酸
Mohammad Rifqi Ghiffary,Fong Tian Wong,Yee Hwee Lim
Mohammad Rifqi Ghiffary
In the last decade, the global warming and plastic pollution issue have driven research on developing a more sustainable platform for chemicals production from alternative feedstocks. Ethylene glycol (EG), a monomer of polyethylene terephth...
Mapping Epoxyquinoid Biosynthesis: Enzyme Functions Across Bacteria and Fungi [0.03%]
表征环氧化咯酮生物合成途径：细菌和真菌中的酶功能研究
Hsin-Yu Wei,Wei-Hsuan Lin,Hsiao-Ching Lin
Hsin-Yu Wei
Epoxyquinoids are a unique class of natural products featuring an epoxide embedded within a quinone/quinol scaffold, typically as an epoxycyclohexenone (ECH). Their striking stereochemical diversity arises from multiple permutations of epox...
Bioprospecting and Phylogenetic Analysis of Priestia flexa AW3: An Industrially Significant Amylase-Producing Bacterium from Unexplored Contaminated Soil in Layyah [0.03%]
拉亚未探索的污染土壤中具有工业价值的淀粉酶产生菌Priestia flexa AW3 的生物勘探和系统发育分析
Sobia Ramzan,Musrat Shaheen,Mohsin Khurshid et al.
Sobia Ramzan et al.
In spite of wonderful industrial applications of microbial enzymes, still most of habitats in various parts of world are unexplored for bioprospecting of industrial potent microbes. This study represents the first bioprospecting effort in L...
Sustainable β-Carotene Production from Sorghum Syrup Using Rhodotorula glutinis: Bioprocess Optimization and Scale-Up [0.03%]
利用红酵母Rhodotorula glutinis从高粱糖浆中生产可持续β-胡萝卜素：发酵工艺优化及放大研究
Kevaughn Prout,Keerthi Mandyam,Ananda Nanjundaswamy
Kevaughn Prout
β-Carotene, a key provitamin A carotenoid, is widely used as an antioxidant and natural pigment. Due to animals' inability to synthesize carotenoids, dietary sources are essential. This study utilized low-cost sorghum syrup for β-carotene...