Harnessing regulatory networks in Actinobacteria for natural product discovery [0.03%]
利用放线菌中的调控网络进行天然产物的发现
Hannah E Augustijn,Anna M Roseboom,Marnix H Medema et al.
Hannah E Augustijn et al.
Microbes typically live in complex habitats where they need to rapidly adapt to continuously changing growth conditions. To do so, they produce an astonishing array of natural products with diverse structures and functions. Actinobacteria s...
Using design of experiments to guide genetic optimization of engineered metabolic pathways [0.03%]
使用实验设计来指导基因优化的工程代谢途径
Seonyun Moon,Anna Saboe,Michael J Smanski
Seonyun Moon
Design of experiments (DoE) is a term used to describe the application of statistical approaches to interrogate the impact of many variables on the performance of a multivariate system. It is commonly used for process optimization in fields...
CRISPR-aided genome engineering for secondary metabolite biosynthesis in Streptomyces [0.03%]
CRISPR技术辅助链霉菌二级代谢产物生物合成中的基因工程改造
Yongjae Lee,Soonkyu Hwang,Woori Kim et al.
Yongjae Lee et al.
The demand for discovering novel microbial secondary metabolites is growing to address the limitations in bioactivities such as antibacterial, antifungal, anticancer, anthelmintic, and immunosuppressive functions. Among microbes, the genus ...
Expanding the synthetic biology toolbox of Cupriavidus necator for establishing fatty acid production [0.03%]
扩展Cupriavidus necator的合成生物学工具箱以建立脂肪酸生产体系
Shivangi Mishra,Paul M Perkovich,Wayne P Mitchell et al.
Shivangi Mishra et al.
The Gram-negative betaproteobacterium Cupriavidus necator is a chemolithotroph that can convert carbon dioxide into biomass. Cupriavidus necator has been engineered to produce a variety of high-value chemicals in the past. However, there is...
Cultivation of recombinant Aspergillus niger strains on dairy whey as a carbohydrate source [0.03%]
利用乳清作为碳源培养重组黑曲霉菌株
Teagan C Crament,Kayline Arendsen,Shaunita H Rose et al.
Teagan C Crament et al.
Agricultural waste valorisation provides a sustainable solution to waste management, and combining waste utilisation with commodity production allows for responsible production processes. Recombinant Aspergillus niger D15 strains expressing...
Transcriptional and translational flux optimization at the key regulatory node for enhanced production of naringenin using acetate in engineered Escherichia coli [0.03%]
通过乙酸改造大肠杆菌在关键调控节点优化转录和翻译通量从而提高柚皮素的产量
Dong H Kim,Hyun G Hwang,Dae-Yeol Ye et al.
Dong H Kim et al.
As a key molecular scaffold for various flavonoids, naringenin is a value-added chemical with broad pharmaceutical applicability. For efficient production of naringenin from acetate, it is crucial to precisely regulate the carbon flux of th...
Tsung-Han Chao,Xiangyu Wu,Hans Renata
Tsung-Han Chao
Despite their prevalent use in drug discovery and protein biochemistry, non-canonical amino acids are still challenging to synthesize through purely chemical means. In recent years, biocatalysis has emerged as a transformative paradigm for ...
Development of platensimycin, platencin, and platensilin overproducers by biosynthetic pathway engineering and fermentation medium optimization [0.03%]
通过生物合成途径工程和发酵培养基优化开发platensimycin, platencin 和platensilin 的高产菌株
Lucas L Fluegel,Ming-Rong Deng,Ping Su et al.
Lucas L Fluegel et al.
The platensimycin (PTM), platencin (PTN), and platensilin (PTL) family of natural products continues to inspire the discovery of new chemistry, enzymology, and medicine. Engineered production of this emerging family of natural products, how...
Secretory expression of amylosucrase in Bacillus licheniformis through twin-arginine translocation pathway [0.03%]
通过双精氨酸转运途径在嗜热脂孢子菌中分泌表达淀粉蔗糖酶
Caizhe Wang,Dandan Niu,Nokuthula Peace Mchunu et al.
Caizhe Wang et al.
Amylosucrase (EC 2.4.1.4) is a versatile enzyme with significant potential in biotechnology and food production. To facilitate its efficient preparation, a novel expression strategy was implemented in Bacillus licheniformis for the secretor...