Severe COVID-19-associated variants linked to chemokine receptor gene control in monocytes and macrophages [0.03%]
与单核细胞和巨噬细胞中的趋化因子受体基因控制相关的严重COVID-19变异株
Bernard S Stikker,Grégoire Stik,Antoinette F van Ouwerkerk et al.
Bernard S Stikker et al.
Genome-wide association studies have identified 3p21.31 as the main risk locus for severe COVID-19, although underlying mechanisms remain elusive. We perform an epigenomic dissection of 3p21.31, identifying a CTCF-dependent tissue-specific ...
LinDA: linear models for differential abundance analysis of microbiome compositional data [0.03%]
基于微生物组组成数据的差异丰度分析的线性模型LinDA
Huijuan Zhou,Kejun He,Jun Chen et al.
Huijuan Zhou et al.
Differential abundance analysis is at the core of statistical analysis of microbiome data. The compositional nature of microbiome sequencing data makes false positive control challenging. Here, we show that the compositional effects can be ...
Strand asymmetry influences mismatch resolution during a single-strand annealing [0.03%]
链不对称影响单链 annealing 过程中的错配解析
Victoria O Pokusaeva,Aránzazu Rosado Diez,Lorena Espinar et al.
Victoria O Pokusaeva et al.
Background: Biases of DNA repair can shape the nucleotide landscape of genomes at evolutionary timescales. The molecular mechanisms of those biases are still poorly understood because it is difficult to isolate the contri...
Local euchromatin enrichment in lamina-associated domains anticipates their repositioning in the adipogenic lineage [0.03%]
染色质重塑在细胞向脂肪细胞分化过程中的作用
Julia Madsen-Østerbye,Mohamed Abdelhalim,Marie-Odile Baudement et al.
Julia Madsen-Østerbye et al.
Background: Interactions of chromatin with the nuclear lamina via lamina-associated domains (LADs) confer structural stability to the genome. The dynamics of positioning of LADs during differentiation, and how LADs imping...
Expansion of the prime editing modality with Cas9 from Francisella novicida [0.03%]
利用Francisella novicida的Cas9扩展prime编辑体系
Yeounsun Oh,Wi-Jae Lee,Junho K Hur et al.
Yeounsun Oh et al.
Prime editing can induce a desired base substitution, insertion, or deletion in a target gene using reverse transcriptase after nick formation by CRISPR nickase. In this study, we develop a technology that can be used to insert or replace e...
Cell type determination for cardiac differentiation occurs soon after seeding of human-induced pluripotent stem cells [0.03%]
人诱导多能干细胞播种后不久心脏分化细胞类型决定便发生了
Connie L Jiang,Yogesh Goyal,Naveen Jain et al.
Connie L Jiang et al.
Background: Cardiac differentiation of human-induced pluripotent stem (hiPS) cells consistently produces a mixed population of cardiomyocytes and non-cardiac cell types, even when using well-characterized protocols. We so...
The contribution of evolutionarily volatile promoters to molecular phenotypes and human trait variation [0.03%]
演化易变启动子对分子表型和人类性状变异的贡献
Robert S Young,Lana Talmane,Sophie Marion de Procé et al.
Robert S Young et al.
Background: Promoters are sites of transcription initiation that harbour a high concentration of phenotype-associated genetic variation. The evolutionary gain and loss of promoters between species (collectively, termed tu...
Decoding the multicellular ecosystem of vena caval tumor thrombus in clear cell renal cell carcinoma by single-cell RNA sequencing [0.03%]
单细胞RNA测序解码透明细胞肾细胞癌腔静脉瘤栓的多细胞生态系统
Yue Shi,Qi Zhang,Hai Bi et al.
Yue Shi et al.
Background: Vascular invasion with tumor thrombus frequently occurs in advanced renal cell carcinoma (RCC). Thrombectomy is one of the most challenging surgeries with high rate of perioperative morbidity and mortality. Ho...
Aleksandar Janjic,Lucas E Wange,Johannes W Bagnoli et al.
Aleksandar Janjic et al.
Cost-efficient library generation by early barcoding has been central in propelling single-cell RNA sequencing. Here, we optimize and validate prime-seq, an early barcoding bulk RNA-seq method. We show that it performs equivalently to TruSe...
David Mark Emms,Steven Kelly
David Mark Emms
Determining the evolutionary relationships between genes is fundamental to comparative biological research. Here, we present SHOOT. SHOOT searches a user query sequence against a database of phylogenetic trees and returns a tree with the qu...