Folliculin-interacting protein FNIP2 impacts on overweight and obesity through a polymorphism in a conserved 3' untranslated region [0.03%]
新的多态性位点FNIP2通过调控FNIP2表达影响体重和肥胖
Lara P Fernández,Nerea Deleyto-Seldas,Gonzalo Colmenarejo et al.
Lara P Fernández et al.
Background: Overweight and obesity are defined by an anomalous or excessive fat accumulation that may compromise health. To find single-nucleotide polymorphisms (SNPs) influencing metabolic phenotypes associated with the ...
scTAM-seq enables targeted high-confidence analysis of DNA methylation in single cells [0.03%]
scTAM-seq使单细胞DNA甲基化分析具有靶向性和高可信度
Agostina Bianchi,Michael Scherer,Roser Zaurin et al.
Agostina Bianchi et al.
Single-cell DNA methylation profiling currently suffers from excessive noise and/or limited cellular throughput. We developed scTAM-seq, a targeted bisulfite-free method for profiling up to 650 CpGs in up to 10,000 cells per experiment, wit...
INSERT-seq enables high-resolution mapping of genomically integrated DNA using Nanopore sequencing [0.03%]
INSERT-seq利用Nanopore测序技术对基因组整合DNA进行高分辨率映射
Dimitrije Ivančić,Júlia Mir-Pedrol,Jessica Jaraba-Wallace et al.
Dimitrije Ivančić et al.
Comprehensive characterisation of genome engineering technologies is relevant for their development and safe use in human gene therapy. Short-read based methods can overlook insertion events in repetitive regions. We develop INSERT-seq, a m...
ReadZS detects cell type-specific and developmentally regulated RNA processing programs in single-cell RNA-seq [0.03%]
ReadZS检测单细胞RNA测序中的细胞类型特异性及发育过程中的RNA加工程序
Elisabeth Meyer,Kaitlin Chaung,Roozbeh Dehghannasiri et al.
Elisabeth Meyer et al.
RNA processing, including splicing and alternative polyadenylation, is crucial to gene function and regulation, but methods to detect RNA processing from single-cell RNA sequencing data are limited by reliance on pre-existing annotations, p...
CRISPR-KRISPR: a method to identify on-target and random insertion of donor DNAs and their characterization in knock-in mice [0.03%]
CRISPR-KRISPR方法鉴定基因组靶位点及随机位点的外源DNA整合并表征敲入小鼠中的插入片段
Masayuki Tanaka,Keiko Yokoyama,Hideki Hayashi et al.
Masayuki Tanaka et al.
CRISPR tools can generate knockout and knock-in animal models easily, but the models can contain off-target genomic lesions or random insertions of donor DNAs. Simpler methods to identify off-target lesions and random insertions, using tail...
Significant variation in the performance of DNA methylation predictors across data preprocessing and normalization strategies [0.03%]
DNA甲基化预测在数据预处理和规范化策略上的表现差异较大
Anil P S Ori,Ake T Lu,Steve Horvath et al.
Anil P S Ori et al.
Background: DNA methylation (DNAm)-based predictors hold great promise to serve as clinical tools for health interventions and disease management. While these algorithms often have high prediction accuracy, the consistenc...
Author Correction: Pooled extracellular receptor-ligand interaction screening using CRISPR activation [0.03%]
作者更正：使用CRISPR激活的汇集型细胞外受体-配体相互作用筛选
Zheng-Shan Chong,Shuhei Ohnishi,Kosuke Yusa et al.
Zheng-Shan Chong et al.
Published Erratum
Genome biology. 2022 Oct 21;23(1):224. DOI:10.1186/s13059-022-02797-6 2022
Replication stress generates distinctive landscapes of DNA copy number alterations and chromosome scale losses [0.03%]
复制压力产生独特的DNA拷贝数改变和染色体规模损失景观
Nadeem Shaikh,Alice Mazzagatti,Simone De Angelis et al.
Nadeem Shaikh et al.
Background: A major driver of cancer chromosomal instability is replication stress, the slowing or stalling of DNA replication. How replication stress and genomic instability are connected is not known. Aphidicolin-induce...
Local CpG density affects the trajectory and variance of age-associated DNA methylation changes [0.03%]
局部CpG密度影响年龄相关DNA甲基化变化的轨迹和方差
Jonathan Higham,Lyndsay Kerr,Qian Zhang et al.
Jonathan Higham et al.
Background: DNA methylation is an epigenetic mark associated with the repression of gene promoters. Its pattern in the genome is disrupted with age and these changes can be used to statistically predict age with epigeneti...
A quantitative metric of pioneer activity reveals that HNF4A has stronger in vivo pioneer activity than FOXA1 [0.03%]
量化先驱活性指标显示HNF4A的体内先驱活性强于FOXA1
Jeffrey L Hansen,Barak A Cohen
Jeffrey L Hansen
Background: We and others have suggested that pioneer activity - a transcription factor's (TF's) ability to bind and open inaccessible loci - is not a qualitative trait limited to a select class of pioneer TFs. We hypothe...