Target residence of Cas9-sgRNA influences DNA double-strand break repair pathway choices in CRISPR/Cas9 genome editing [0.03%]
CRISPR/Cas9基因编辑中Cas9-sgRNA的作用靶点影响DNA双链断裂的修复路径选择
Si-Cheng Liu,Yi-Li Feng,Xiu-Na Sun et al.
Si-Cheng Liu et al.
Background: Due to post-cleavage residence of the Cas9-sgRNA complex at its target, Cas9-induced DNA double-strand breaks (DSBs) have to be exposed to engage DSB repair pathways. Target interaction of Cas9-sgRNA determine...
Author Correction: High-throughput techniques enable advances in the roles of DNA and RNA secondary structures in transcriptional and post-transcriptional gene regulation [0.03%]
作者更正：高通量技术使人们能够推进对DNA和RNA二级结构在转录和转录后基因调控中的作用的理解
Ilias Georgakopoulos-Soares,Candace S Y Chan,Nadav Ahituv et al.
Ilias Georgakopoulos-Soares et al.
Published Erratum
Genome biology. 2022 Jul 27;23(1):164. DOI:10.1186/s13059-022-02733-8 2022
Large-scale manipulation of promoter DNA methylation reveals context-specific transcriptional responses and stability [0.03%]
大规模操纵启动子DNA甲基化揭示了特定环境的转录反应和稳定性
Alex de Mendoza,Trung Viet Nguyen,Ethan Ford et al.
Alex de Mendoza et al.
Background: Cytosine DNA methylation is widely described as a transcriptional repressive mark with the capacity to silence promoters. Epigenome engineering techniques enable direct testing of the effect of induced DNA met...
Juan Li,Like Chen,Jing Liang et al.
Juan Li et al.
Low efficiency has seriously restricted the application of prime editing (PE) systems in plants. In this study, we develop an enhanced plant prime editor 2 system, enpPE2, by stacking various optimization strategies, including updating the ...
ZetaSuite: computational analysis of two-dimensional high-throughput data from multi-target screens and single-cell transcriptomics [0.03%]
ZetaSuite：来自多靶标筛选和单细胞转录组学的二维高通量数据的计算分析
Yajing Hao,Shuyang Zhang,Changwei Shao et al.
Yajing Hao et al.
Two-dimensional high-throughput data have become increasingly common in functional genomics studies, which raises new challenges in data analysis. Here, we introduce a new statistic called Zeta, initially developed to identify global splici...
Integration of single-cell multi-omics data by regression analysis on unpaired observations [0.03%]
基于未配对样本的回归分析集成单细胞多组学数据
Qiuyue Yuan,Zhana Duren
Qiuyue Yuan
Despite recent developments, it is hard to profile all multi-omics single-cell data modalities on the same cell. Thus, huge amounts of single-cell genomics data of unpaired observations on different cells are generated. We propose a method ...
High-throughput techniques enable advances in the roles of DNA and RNA secondary structures in transcriptional and post-transcriptional gene regulation [0.03%]
高通量技术助力DNA和RNA二级结构在转录及转录后基因调控中的作用研究取得进展
Ilias Georgakopoulos-Soares,Candace S Y Chan,Nadav Ahituv et al.
Ilias Georgakopoulos-Soares et al.
The most stable structure of DNA is the canonical right-handed double helix termed B DNA. However, certain environments and sequence motifs favor alternative conformations, termed non-canonical secondary structures. The roles of DNA and RNA...
Detecting cell-of-origin and cancer-specific methylation features of cell-free DNA from Nanopore sequencing [0.03%]
基于纳米孔测序的细胞游离DNA的细胞起源及肿瘤特异性甲基化特征检测
Efrat Katsman,Shari Orlanski,Filippo Martignano et al.
Efrat Katsman et al.
The Oxford Nanopore (ONT) platform provides portable and rapid genome sequencing, and its ability to natively profile DNA methylation without complex sample processing is attractive for point-of-care real-time sequencing. We recently demons...
Correction: Refining colorectal cancer classification and clinical stratification through a single-cell atlas [0.03%]
纠正：通过单细胞图谱完善结直肠癌的分类和临床分层
Ateeq M Khaliq,Cihat Erdogan,Zeyneb Kurt et al.
Ateeq M Khaliq et al.
Published Erratum
Genome biology. 2022 Jul 13;23(1):156. DOI:10.1186/s13059-022-02724-9 2022
Publisher Correction: Bookend: precise transcript reconstruction with end-guided assembly [0.03%]
出版更正：Bookend：末端引导组装的精确转录重建
Michael A Schon,Stefan Lutzmayer,Falko Hofmann et al.
Michael A Schon et al.
Published Erratum
Genome biology. 2022 Jul 13;23(1):157. DOI:10.1186/s13059-022-02725-8 2022