A comparison of marker gene selection methods for single-cell RNA sequencing data [0.03%]
单细胞RNA测序数据中标志基因选择方法的比较
Jeffrey M Pullin,Davis J McCarthy
Jeffrey M Pullin
Background: The development of single-cell RNA sequencing (scRNA-seq) has enabled scientists to catalog and probe the transcriptional heterogeneity of individual cells in unprecedented detail. A common step in the analysi...
HiHo-AID2: boosting homozygous knock-in efficiency enables robust generation of human auxin-inducible degron cells [0.03%]
嗨嗬AID2：提高纯合敲入效率以实现稳定生成人类吲哚乙酸可诱导降解细胞系统
Shiqian Li,Yafei Wang,Miesje van der Stoel et al.
Shiqian Li et al.
Recent developments in auxin-inducible degron (AID) technology have increased its popularity for chemogenetic control of proteolysis. However, generation of human AID cell lines is challenging, especially in human embryonic stem cells (hESC...
Precise fine-turning of GhTFL1 by base editing tools defines ideal cotton plant architecture [0.03%]
基于编辑工具对GhTFL1的精确微调定义了理想的棉花植株结构
Guanying Wang,Fuqiu Wang,Zhongping Xu et al.
Guanying Wang et al.
Background: CRISPR/Cas-derived base editor enables precise editing of target sites and has been widely used for basic research and crop genetic improvement. However, the editing efficiency of base editors at different tar...
scGIST: gene panel design for spatial transcriptomics with prioritized gene sets [0.03%]
scGIST：基于优先基因集的空间转录组学基因panel设计
Mashrur Ahmed Yafi,Md Hasibul Husain Hisham,Francisco Grisanti et al.
Mashrur Ahmed Yafi et al.
A critical challenge of single-cell spatial transcriptomics (sc-ST) technologies is their panel size. Being based on fluorescence in situ hybridization, they are typically limited to panels of about a thousand genes. This constrains researc...
SHARE-Topic: Bayesian interpretable modeling of single-cell multi-omic data [0.03%]
分享主题：单细胞多组学数据的贝叶斯可解释建模
Nour El Kazwini,Guido Sanguinetti
Nour El Kazwini
Multi-omic single-cell technologies, which simultaneously measure the transcriptional and epigenomic state of the same cell, enable understanding epigenetic mechanisms of gene regulation. However, noisy and sparse data pose fundamental stat...
CAGI, the Critical Assessment of Genome Interpretation, establishes progress and prospects for computational genetic variant interpretation methods [0.03%]
基因组解读关键评估(CAGI)建立计算遗传变异解读方法的进展和前景
Critical Assessment of Genome Interpretation Consortium
Critical Assessment of Genome Interpretation Consortium
Background: The Critical Assessment of Genome Interpretation (CAGI) aims to advance the state-of-the-art for computational prediction of genetic variant impact, particularly where relevant to disease. The five complete ed...
Tianru Zhang,Changhao Li,Jiaying Zhu et al.
Tianru Zhang et al.
Background: RNA secondary structure (RSS) can influence the regulation of transcription, RNA processing, and protein synthesis, among other processes. 3' untranslated regions (3' UTRs) of mRNA also hold the key for many a...
Undetectable off-target effects induced by FokI catalytic domain in mouse embryos [0.03%]
由FokI催化域在小鼠胚胎中诱导的无法检测到的脱靶效应
Long Xie,Hu Feng,Zhifang Li et al.
Long Xie et al.
The FokI catalytic domain can be fused to various DNA binding architectures to improve the precision of genome editing tools. However, evaluation of off-target effects is essential for developing these tools. We use Genome-wide Off-target a...
DNA satellite and chromatin organization at mouse centromeres and pericentromeres [0.03%]
小鼠着丝粒和近着丝粒的DNA卫星及染色质构象
Jenika Packiaraj,Jitendra Thakur
Jenika Packiaraj
Background: Centromeres are essential for faithful chromosome segregation during mitosis and meiosis. However, the organization of satellite DNA and chromatin at mouse centromeres and pericentromeres is poorly understood ...
Author Correction: Cross-phyla protein annotation by structural prediction and alignment [0.03%]
作者更正：通过结构预测和比对进行跨门蛋白注释
Fabian Ruperti,Nikolaos Papadopoulos,Jacob M Musser et al.
Fabian Ruperti et al.
Published Erratum
Genome biology. 2024 Feb 19;25(1):50. DOI:10.1186/s13059-024-03196-9 2024