Long-term DNA methylation changes mediate heterologous cytokine responses after BCG vaccination [0.03%]
卡介苗接种后的长期DNA甲基化变化可介导异源细胞因子反应
Cancan Qi,Zhaoli Liu,Gizem Kilic et al.
Cancan Qi et al.
Background: Epigenetic reprogramming shapes immune memory in both innate (trained immunity) and adaptive immune cells following Bacillus Calmette-Guérin (BCG) vaccination. However, the role of dynamic DNA methylation cha...
Comprehensive protein datasets and benchmarking for liquid-liquid phase separation studies [0.03%]
用于液-液相分离研究的全面蛋白质数据集及基准测试
Carlos Pintado-Grima,Oriol Bárcenas,Eva Arribas-Ruiz et al.
Carlos Pintado-Grima et al.
Background: Proteins self-organize in dynamic cellular environments by assembling into reversible biomolecular condensates through liquid-liquid phase separation (LLPS). These condensates can comprise single or multiple p...
Cell cycle-dependent TICRR/TRESLIN and MTBP chromatin binding mechanisms and patterns [0.03%]
细胞周期依赖的TICRR/TRESLIN和MTBP染色质结合机制与模式
Tyler D Noble,Courtney G Sansam,Kimberlie A Wittig et al.
Tyler D Noble et al.
Background: The selection of replication origins is a defining characteristic of DNA replication in eukaryotes, yet its mechanism in humans has not been well defined. Potential DNA replication origins are licensed through...
A genome-wide association study integrated with single-cell and bulk profiles uncovers susceptibility genes for nasopharyngeal carcinoma involved in tumorigenesis via regulation of T cells [0.03%]
整合单细胞和批量谱型的全基因组关联研究发现鼻咽癌致病易感基因
Tong-Min Wang,Wen-Li Zhang,Jin-Ru Xie et al.
Tong-Min Wang et al.
Background: Nasopharyngeal carcinoma is an aggressive malignancy originating from the nasopharyngeal mucosa and associated with genetic factors. Many nasopharyngeal carcinoma susceptibility loci have been identified by ge...
Conserved missense variant pathogenicity and correlated phenotypes across paralogous genes [0.03%]
同源基因中保守的错义变异致病性及其相关表型
Tobias Brünger,Alina Ivaniuk,Eduardo Pérez-Palma et al.
Tobias Brünger et al.
Background: The majority of missense variants in clinical genetic tests are classified as variants of uncertain significance. Prior research shows that the deleterious effects and the subsequent molecular consequences of ...
IT-scC&T-seq streamlines scalable, parallel profiling of protein-DNA interactions in single cells [0.03%]
IT-scCO&T-seq简化了单细胞中蛋白质- DNA相互作用的可扩展并行谱型分析
Jingchun Ma,Wei Jin,Li Rong et al.
Jingchun Ma et al.
Single-cell profiling protein-chromatin interactions is often constrained by complex workflows, high cost, or dependence on specialized equipment. We present indexed tagmentation-based single-cell CUT&Tag-sequencing (IT-scC&T-seq), a modula...
Systematic assessment of microenvironment-dependent transcriptional patterns and intercellular communication [0.03%]
系统评估微环境依赖性转录模式和细胞间通讯
Elena Pareja-Lorente,Patrick Aloy
Elena Pareja-Lorente
Background: Understanding cell-cell communication and its dependence on spatial organization is critical for unraveling tissue complexity and organ function, and it has been demonstrated that cells are influenced by their...
A new IDH-independent hypermethylation phenotype is associated with astrocyte-like cell state in glioblastoma [0.03%]
IDH阴性的新型高甲基化表型与胶质母细胞瘤中的星形胶质细胞状态相关
Ana Luisa Costa,Daria Doncevic,Yonghe Wu et al.
Ana Luisa Costa et al.
Background: DNA methylation plays a crucial role in cancer development and progression and has been linked to genetically and clinically distinct tumor classes, including IDH-mutated and IDH-wildtype adult-type diffuse gl...
Ensemblex: an accuracy-weighted ensemble genetic demultiplexing framework for population-scale scRNAseq sample pooling [0.03%]
基于群体规模的单细胞测序样本池化遗传解复用的准确性加权集成框架（Ensemblex）
Michael R Fiorini,Saeid Amiri,Allison A Dilliott et al.
Michael R Fiorini et al.
Multiplexing samples from distinct individuals prior to sequencing is a promising step towards achieving population-scale single-cell RNA sequencing by reducing the restrictive costs of the technology. Individual genetic demultiplexing tool...
Optimized in-solution enrichment of over a million ancient human SNPs [0.03%]
优化古人类过百万单核苷酸多态位点的溶液捕获方法
Roberta Davidson,Xavier Roca-Rada,Shyamsundar Ravishankar et al.
Roberta Davidson et al.
Background: In-solution hybridization enrichment of genetic markers is a method of choice in paleogenomic studies, where the DNA of interest is generally heavily fragmented and contaminated with environmental DNA, and whe...