A pangenome analysis pipeline provides insights into functional gene identification in rice [0.03%]
一种泛基因组分析管道可提供水稻功能基因鉴定见解
Jian Wang,Wu Yang,Shaohong Zhang et al.
Jian Wang et al.
Background: A pangenome aims to capture the complete genetic diversity within a species and reduce bias in genetic analysis inherent in using a single reference genome. However, the current linear format of most plant pan...
Auxin-inducible degron 2 system deciphers functions of CTCF domains in transcriptional regulation [0.03%]
组蛋白边界因子CTCF的功能结构域及其转录调控作用
Judith Hyle,Mohamed Nadhir Djekidel,Justin Williams et al.
Judith Hyle et al.
Background: CTCF is a well-established chromatin architectural protein that also plays various roles in transcriptional regulation. While CTCF biology has been extensively studied, how the domains of CTCF function to regu...
β-actin mediated H3K27ac changes demonstrate the link between compartment switching and enhancer-dependent transcriptional regulation [0.03%]
β-actin介导的H3K27ac变化表明了隔室转换和增强子依赖的转录调控之间的联系
Syed Raza Mahmood,Nadine Hosny El Said,Kristin C Gunsalus et al.
Syed Raza Mahmood et al.
Background: Recent work has demonstrated that three-dimensional genome organization is directly affected by changes in the levels of nuclear cytoskeletal proteins such as β-actin. The mechanisms which translate changes i...
Zhongyu Zou,Caraline Sepich-Poore,Xiaoming Zhou et al.
Zhongyu Zou et al.
The YTH N6-methyladenosine RNA binding proteins (YTHDFs) mediate the functional effects of N6-methyladenosine (m6A) on RNA. Recently, a report proposed that all YTHDFs work redundantly to facilitate RNA decay, raising questions about the ex...
An atlas of lamina-associated chromatin across twelve human cell types reveals an intermediate chromatin subtype [0.03%]
涵盖十二种人类细胞类型的染色质层粘连复合物图谱揭示了一种中间的染色质亚型
Parisha P Shah,Kathleen C Keough,Ketrin Gjoni et al.
Parisha P Shah et al.
Background: Association of chromatin with lamin proteins at the nuclear periphery has emerged as a potential mechanism to coordinate cell type-specific gene expression and maintain cellular identity via gene silencing. Un...
Anjana C Karawita,Yuanyuan Cheng,Keng Yih Chew et al.
Anjana C Karawita et al.
Background: The Australian black swan (Cygnus atratus) is an iconic species with contrasting plumage to that of the closely related northern hemisphere white swans. The relative geographic isolation of the black swan may ...
Pan-3D genome analysis reveals structural and functional differentiation of soybean genomes [0.03%]
泛-3D基因组分析揭示了大豆基因组的结构和功能分化
Lingbin Ni,Yucheng Liu,Xin Ma et al.
Lingbin Ni et al.
Background: High-order chromatin structure plays important roles in gene regulation. However, the diversity of the three-dimensional (3D) genome across plant accessions are seldom reported. ...
Accounting for cis-regulatory constraint prioritizes genes likely to affect species-specific traits [0.03%]
考虑顺式调控约束以优先选择可能影响物种特性的基因
Alexander L Starr,David Gokhman,Hunter B Fraser
Alexander L Starr
Measuring allele-specific expression in interspecies hybrids is a powerful way to detect cis-regulatory changes underlying adaptation. However, it remains difficult to identify genes most likely to explain species-specific traits. Here, we ...
CRISPR-dCas13-tracing reveals transcriptional memory and limited mRNA export in developing zebrafish embryos [0.03%]
CRISPR-dCas13-tracing揭示发育中的斑马鱼胚胎中转录记忆和有限的mRNA输出
Youkui Huang,Bao-Qing Gao,Quan Meng et al.
Youkui Huang et al.
Background: Understanding gene transcription and mRNA-protein (mRNP) dynamics in single cells in a multicellular organism has been challenging. The catalytically dead CRISPR-Cas13 (dCas13) system has been used to visualiz...
Enhanced RNA knockdown efficiency with engineered fusion guide RNAs that function with both CRISPR-CasRx and hammerhead ribozyme [0.03%]
工程融合导向RNA与CRISPR-CasRx和锤头核酶共同作用，提高RNA敲低效率
Yonghao Zhan,Congcong Cao,Aolin Li et al.
Yonghao Zhan et al.
Background: CRISPR-Cas13 is a newly emerging RNA knockdown technology that is comparable to RNAi. Among all members of Cas13, CasRx degrades RNA in human cells with high precision and effectiveness. However, it remains un...