PhaseDancer: a novel targeted assembler of segmental duplications unravels the complexity of the human chromosome 2 fusion going from 48 to 46 chromosomes in hominin evolution [0.03%]
相位舞者：一种新颖的靶向重复序列组装程序揭示了从四十八到四十六染色体的人类二号染色体融合复杂性
Barbara Poszewiecka,Krzysztof Gogolewski,Justyna A Karolak et al.
Barbara Poszewiecka et al.
Resolving complex genomic regions rich in segmental duplications (SDs) is challenging due to the high error rate of long-read sequencing. Here, we describe a targeted approach with a novel genome assembler PhaseDancer that extends SD-rich r...
The relationship between regulatory changes in cis and trans and the evolution of gene expression in humans and chimpanzees [0.03%]
人类和黑猩猩顺式和反式调控变化与基因表达进化的关系研究
Kenneth A Barr,Katherine L Rhodes,Yoav Gilad
Kenneth A Barr
Background: Comparative gene expression studies in apes are fundamentally limited by the challenges associated with sampling across different tissues. Here, we used single-cell RNA sequencing of embryoid bodies to collect...
ZINBMM: a general mixture model for simultaneous clustering and gene selection using single-cell transcriptomic data [0.03%]
基于单细胞转录组数据的同时聚类和基因选择的通用混合模型(ZINBMM)
Yang Li,Mingcong Wu,Shuangge Ma et al.
Yang Li et al.
Clustering is a critical component of single-cell RNA sequencing (scRNA-seq) data analysis and can help reveal cell types and infer cell lineages. Despite considerable successes, there are few methods tailored to investigating cluster-speci...
Artur Jaroszewicz,Jason Ernst
Artur Jaroszewicz
Various computational approaches have been developed to annotate epigenomes on a per-position basis by modeling combinatorial and spatial patterns within epigenomic data. However, such annotations are less suitable for gene-based analyses. ...
Quartet protein reference materials and datasets for multi-platform assessment of label-free proteomics [0.03%]
基于无标记蛋白质组学多平台评估的四重奏蛋白参考材料和数据集
Sha Tian,Dongdong Zhan,Ying Yu et al.
Sha Tian et al.
Background: Quantitative proteomics is an indispensable tool in life science research. However, there is a lack of reference materials for evaluating the reproducibility of label-free liquid chromatography-tandem mass spe...
Correcting batch effects in large-scale multiomics studies using a reference-material-based ratio method [0.03%]
利用参考材料的比率法校正大规模多组学研究中的批次效应
Ying Yu,Naixin Zhang,Yuanbang Mai et al.
Ying Yu et al.
Background: Batch effects are notoriously common technical variations in multiomics data and may result in misleading outcomes if uncorrected or over-corrected. A plethora of batch-effect correction algorithms are propose...
Pervasive under-dominance in gene expression underlying emergent growth trajectories in Arabidopsis thaliana hybrids [0.03%]
拟南芥杂种生长轨迹中普遍存在的转录潜隐现象
Wei Yuan,Fiona Beitel,Thanvi Srikant et al.
Wei Yuan et al.
Background: Complex traits, such as growth and fitness, are typically controlled by a very large number of variants, which can interact in both additive and non-additive fashion. In an attempt to gauge the relative import...
Single-cell transcriptomics reveals multiple chemoresistant properties in leukemic stem and progenitor cells in pediatric AML [0.03%]
单细胞转录组揭示了儿童急性髓系白血病干细胞和祖细胞中的多种化疗耐药属性
Yongping Zhang,Shuting Jiang,Fuhong He et al.
Yongping Zhang et al.
Background: Cancer patients can achieve dramatic responses to chemotherapy yet retain resistant tumor cells, which ultimately results in relapse. Although xenograft model studies have identified several cellular and molec...
A DNA adenine demethylase impairs PRC2-mediated repression of genes marked by a specific chromatin signature [0.03%]
DNA腺嘌呤去甲基化酶通过特定染色质标志物阻碍PRC2介导的基因抑制作用
Qingxiao Jia,Xinran Zhang,Qian Liu et al.
Qingxiao Jia et al.
Background: The Fe (II)- and α-ketoglutarate-dependent AlkB family dioxygenases are implicated in nucleotide demethylation. AlkB homolog1 (ALKBH1) is shown to demethylate DNA adenine methylation (6mA) preferentially from...
Lauren Mak,Dmitry Meleshko,David C Danko et al.
Lauren Mak et al.
Synthetic long read sequencing techniques such as UST's TELL-Seq and Loop Genomics' LoopSeq combine 3[Formula: see text] barcoding with standard short-read sequencing to expand the range of linkage resolution from hundreds to tens of thousa...