Ki-67 is necessary during DNA replication for fork protection and genome stability [0.03%]
Ki-67在DNA复制过程中对于叉保护和基因组稳定性是必要的
Konstantinos Stamatiou,Florentin Huguet,Lukas V Serapinas et al.
Konstantinos Stamatiou et al.
Background: The proliferation antigen Ki-67 has been widely used in clinical settings for cancer staging for many years, but investigations on its biological functions have lagged. Recently, Ki-67 has been shown to regula...
spVC for the detection and interpretation of spatial gene expression variation [0.03%]
用于检测和解释空间基因表达变异的spVC方法
Shan Yu,Wei Vivian Li
Shan Yu
Spatially resolved transcriptomics technologies have opened new avenues for understanding gene expression heterogeneity in spatial contexts. However, existing methods for identifying spatially variable genes often focus solely on statistica...
Lu Pan,Paolo Parini,Roman Tremmel et al.
Lu Pan et al.
Single-cell sequencing datasets are key in biology and medicine for unraveling insights into heterogeneous cell populations with unprecedented resolution. Here, we construct a single-cell multi-omics map of human tissues through in-depth ch...
RBM22 regulates RNA polymerase II 5' pausing, elongation rate, and termination by coordinating 7SK-P-TEFb complex and SPT5 [0.03%]
RBM22通过协调7SK-P-TEFb复合物和SPT5调节RNA聚合酶II的5'暂停、延长速度及终止作用
Xian Du,Wenying Qin,Chunyu Yang et al.
Xian Du et al.
Background: Splicing factors are vital for the regulation of RNA splicing, but some have also been implicated in regulating transcription. The underlying molecular mechanisms of their involvement in transcriptional proces...
Minimum information and guidelines for reporting a multiplexed assay of variant effect [0.03%]
变异效应多重测定的报告最低信息和指南
Melina Claussnitzer,Victoria N Parikh,Alex H Wagner et al.
Melina Claussnitzer et al.
Multiplexed assays of variant effect (MAVEs) have emerged as a powerful approach for interrogating thousands of genetic variants in a single experiment. The flexibility and widespread adoption of these techniques across diverse disciplines ...
Measuring, visualizing, and diagnosing reference bias with biastools [0.03%]
利用biastools测量、可视化和诊断引用偏差
Mao-Jan Lin,Sheila Iyer,Nae-Chyun Chen et al.
Mao-Jan Lin et al.
Many bioinformatics methods seek to reduce reference bias, but no methods exist to comprehensively measure it. Biastools analyzes and categorizes instances of reference bias. It works in various scenarios: when the donor's variants are know...
Dharmesh D Bhuva,Chin Wee Tan,Agus Salim et al.
Dharmesh D Bhuva et al.
Spatial molecular data has transformed the study of disease microenvironments, though, larger datasets pose an analytics challenge prompting the direct adoption of single-cell RNA-sequencing tools including normalization methods. Here, we d...
Characterizing glucokinase variant mechanisms using a multiplexed abundance assay [0.03%]
利用多重丰度测定法表征葡萄糖激酶变异体的机制
Sarah Gersing,Thea K Schulze,Matteo Cagiada et al.
Sarah Gersing et al.
Background: Amino acid substitutions can perturb protein activity in multiple ways. Understanding their mechanistic basis may pinpoint how residues contribute to protein function. Here, we characterize the mechanisms unde...
Powerful and accurate detection of temporal gene expression patterns from multi-sample multi-stage single-cell transcriptomics data with TDEseq [0.03%]
利用TDEseq从多样本多阶段单细胞转录组数据中检测强大且准确的时空基因表达模式
Yue Fan,Lei Li,Shiquan Sun
Yue Fan
We present a non-parametric statistical method called TDEseq that takes full advantage of smoothing splines basis functions to account for the dependence of multiple time points in scRNA-seq studies, and uses hierarchical structure linear a...
Benchmarking bioinformatic virus identification tools using real-world metagenomic data across biomes [0.03%]
基于真实世界跨生境宏基因组数据的生物信息学病毒识别工具评测
Ling-Yi Wu,Yasas Wijesekara,Gonçalo J Piedade et al.
Ling-Yi Wu et al.
Background: As most viruses remain uncultivated, metagenomics is currently the main method for virus discovery. Detecting viruses in metagenomic data is not trivial. In the past few years, many bioinformatic virus identif...