Mosaic loss of Y chromosome is associated with aging and epithelial injury in chronic kidney disease [0.03%]
Y染色体的马赛克丢失与慢性肾病中衰老和上皮损伤有关
Parker C Wilson,Amit Verma,Yasuhiro Yoshimura et al.
Parker C Wilson et al.
Background: Mosaic loss of Y chromosome (LOY) is the most common chromosomal alteration in aging men. Here, we use single-cell RNA and ATAC sequencing to show that LOY is present in the kidney and increases with age and c...
Yida Zhang,Viktor Petukhov,Evan Biederstedt et al.
Yida Zhang et al.
Targeted spatial transcriptomics hold particular promise in analyzing complex tissues. Most such methods, however, measure only a limited panel of transcripts, which need to be selected in advance to inform on the cell types or processes be...
The unusual gene architecture of polyubiquitin is created by dual-specific splice sites [0.03%]
多聚泛素的不寻常基因结构是由双特异性剪接位点构建的
Chaorui Duan,Truman Mooney,Luke Buerer et al.
Chaorui Duan et al.
Background: The removal of introns occurs through the splicing of a 5' splice site (5'ss) with a 3' splice site (3'ss). These two elements are recognized by distinct components of the spliceosome. However, introns in high...
Quartet metabolite reference materials for inter-laboratory proficiency test and data integration of metabolomics profiling [0.03%]
用于代谢组学分析的四重代谢物参考物质以进行实验室间比对和数据整合
Naixin Zhang,Qiaochu Chen,Peipei Zhang et al.
Naixin Zhang et al.
Background: Various laboratory-developed metabolomic methods lead to big challenges in inter-laboratory comparability and effective integration of diverse datasets. ...
FMRP-mediated spatial regulation of physiologic NMD targets in neuronal cells [0.03%]
神经细胞中生理NMD靶标的定位调控介导于FMRP
Tatsuaki Kurosaki,Xavier Rambout,Lynne E Maquat
Tatsuaki Kurosaki
In non-polarized cells, nonsense-mediated mRNA decay (NMD) generally begins during the translation of newly synthesized mRNAs after the mRNAs are exported to the cytoplasm. Binding of the FMRP translational repressor to UPF1 on NMD targets ...
Unraveling the phylogenomic diversity of Methanomassiliicoccales and implications for mitigating ruminant methane emissions [0.03%]
揭秘Methanomassiliicoccales的系统基因组多样性及其对缓解反刍动物甲烷排放的意义
Fei Xie,Shengwei Zhao,Xiaoxiu Zhan et al.
Fei Xie et al.
Background: Methanomassiliicoccales are a recently identified order of methanogens that are diverse across global environments particularly the gastrointestinal tracts of animals; however, their metabolic capacities are d...
SURGE: uncovering context-specific genetic-regulation of gene expression from single-cell RNA sequencing using latent-factor models [0.03%]
_SURGE：使用潜因子模型从单细胞RNA测序中揭示特定环境下的遗传调控规律_
Benjamin J Strober,Karl Tayeb,Joshua Popp et al.
Benjamin J Strober et al.
Genetic regulation of gene expression is a complex process, with genetic effects known to vary across cellular contexts such as cell types and environmental conditions. We developed SURGE, a method for unsupervised discovery of context-spec...
Structural variation and DNA methylation shape the centromere-proximal meiotic crossover landscape in Arabidopsis [0.03%]
结构变异和DNA甲基化塑造了拟南芥着丝粒附近减数分裂交换图谱
Joiselle B Fernandes,Matthew Naish,Qichao Lian et al.
Joiselle B Fernandes et al.
Background: Centromeres load kinetochore complexes onto chromosomes, which mediate spindle attachment and allow segregation during cell division. Although centromeres perform a conserved cellular function, their underlyin...
PICALO: principal interaction component analysis for the identification of discrete technical, cell-type, and environmental factors that mediate eQTLs [0.03%]
PICALO：用于识别介导eQTL的离散技术、细胞类型和环境因素的主要交互成分分析
Martijn Vochteloo,Patrick Deelen,Britt Vink;BIOS Consortium;Ellen A Tsai et al.
Martijn Vochteloo et al.
Expression quantitative trait loci (eQTL) offer insights into the regulatory mechanisms of trait-associated variants, but their effects often rely on contexts that are unknown or unmeasured. We introduce PICALO, a method for hidden variable...
DeepVelo: deep learning extends RNA velocity to multi-lineage systems with cell-specific kinetics [0.03%]
DeepVelo：深度学习扩展了多系系统中具有细胞特异性动力学的RNA速度
Haotian Cui,Hassaan Maan,Maria C Vladoiu et al.
Haotian Cui et al.
Existing RNA velocity estimation methods strongly rely on predefined dynamics and cell-agnostic constant transcriptional kinetic rates, assumptions often violated in complex and heterogeneous single-cell RNA sequencing (scRNA-seq) data. Usi...