A comparison of methods for detecting DNA methylation from long-read sequencing of human genomes [0.03%]
比较来自人类基因组合成长读序列的DNA甲基化检测方法
Brynja D Sigurpalsdottir,Olafur A Stefansson,Guillaume Holley et al.
Brynja D Sigurpalsdottir et al.
Background: Long-read sequencing can enable the detection of base modifications, such as CpG methylation, in single molecules of DNA. The most commonly used methods for long-read sequencing are nanopore developed by Oxfor...
Joint analysis of mutational and transcriptional landscapes in human cancer reveals key perturbations during cancer evolution [0.03%]
人癌症中突变和转录景观的联合分析揭示了癌症进化期间的关键扰动
Jae-Won Cho,Jingyi Cao,Martin Hemberg
Jae-Won Cho
Background: Tumors are able to acquire new capabilities, including traits such as drug resistance and metastasis that are associated with unfavorable clinical outcomes. Single-cell technologies have made it possible to st...
Three near-complete genome assemblies reveal substantial centromere dynamics from diploid to tetraploid in Brachypodium genus [0.03%]
三个近乎完整的基因组装配揭示了短柄草属从二倍体到四倍体的染色体中心粒动态变化
Chuanye Chen,Siying Wu,Yishuang Sun et al.
Chuanye Chen et al.
Background: Centromeres are critical for maintaining genomic stability in eukaryotes, and their turnover shapes genome architectures and drives karyotype evolution. However, the co-evolution of centromeres from different ...
Michael P Schneider,Amy E Cullen,Justina Pangonyte et al.
Michael P Schneider et al.
Cancer cells often exhibit DNA copy number aberrations and can vary widely in their ploidy. Correct estimation of the ploidy of single-cell genomes is paramount for downstream analysis. Based only on single-cell DNA sequencing information, ...
Early-life ruminal microbiome-derived indole-3-carboxaldehyde and prostaglandin D2 are effective promoters of rumen development [0.03%]
早年瘤胃微生物群衍生的吲哚-3-甲醛和前列腺素D2是有效的瘤胃发育促进剂
Daming Sun,Gaorui Bian,Kai Zhang et al.
Daming Sun et al.
Background: The function of diverse ruminal microbes is tightly linked to rumen development and host physiology. The system of ruminal microbes is an excellent model to clarify the fundamental ecological relationships amo...
Genomic insight into the origin, domestication, dispersal, diversification and human selection of Tartary buckwheat [0.03%]
苦荞的起源、驯化、传播、分化及人类选择的基因组学研究
Yuqi He,Kaixuan Zhang,Yaliang Shi et al.
Yuqi He et al.
Background: Tartary buckwheat, Fagopyrum tataricum, is a pseudocereal crop with worldwide distribution and high nutritional value. However, the origin and domestication history of this crop remain to be elucidated. ...
Rapid and sensitive detection of genome contamination at scale with FCS-GX [0.03%]
使用FCS-GX进行大规模快速敏感的基因组污染检测
Alexander Astashyn,Eric S Tvedte,Deacon Sweeney et al.
Alexander Astashyn et al.
Assembled genome sequences are being generated at an exponential rate. Here we present FCS-GX, part of NCBI's Foreign Contamination Screen (FCS) tool suite, optimized to identify and remove contaminant sequences in new genomes. FCS-GX scree...
A comparison of marker gene selection methods for single-cell RNA sequencing data [0.03%]
单细胞RNA测序数据中标志基因选择方法的比较
Jeffrey M Pullin,Davis J McCarthy
Jeffrey M Pullin
Background: The development of single-cell RNA sequencing (scRNA-seq) has enabled scientists to catalog and probe the transcriptional heterogeneity of individual cells in unprecedented detail. A common step in the analysi...
HiHo-AID2: boosting homozygous knock-in efficiency enables robust generation of human auxin-inducible degron cells [0.03%]
嗨嗬AID2：提高纯合敲入效率以实现稳定生成人类吲哚乙酸可诱导降解细胞系统
Shiqian Li,Yafei Wang,Miesje van der Stoel et al.
Shiqian Li et al.
Recent developments in auxin-inducible degron (AID) technology have increased its popularity for chemogenetic control of proteolysis. However, generation of human AID cell lines is challenging, especially in human embryonic stem cells (hESC...
Precise fine-turning of GhTFL1 by base editing tools defines ideal cotton plant architecture [0.03%]
基于编辑工具对GhTFL1的精确微调定义了理想的棉花植株结构
Guanying Wang,Fuqiu Wang,Zhongping Xu et al.
Guanying Wang et al.
Background: CRISPR/Cas-derived base editor enables precise editing of target sites and has been widely used for basic research and crop genetic improvement. However, the editing efficiency of base editors at different tar...