Response to: "Merit of integrating in situ transcriptomics and anatomical information for cell annotation and lineage construction in single-cell analyses of Populus" [0.03%]
对“将原位转录组和解剖信息整合用于单细胞杨树分析中的细胞注释和系谱构建的价值”的回应
Shaoming Liang,Yiling Li,Yang Chen et al.
Shaoming Liang et al.
Merit of integrating in situ transcriptomics and anatomical information for cell annotation and lineage construction in single-cell analyses of Populus [0.03%]
基于单细胞分析的杨树细胞注释和系谱构建的原位转录组学和解剖信息整合价值研究
Ying-Lan Chen,Jo-Wei Allison Hsieh,Shang-Che Kuo et al.
Ying-Lan Chen et al.
Cell type annotation and lineage construction are two of the most critical tasks conducted in the analyses of single-cell RNA sequencing (scRNA-seq). Four recent scRNA-seq studies of differentiating xylem propose four models on differentiat...
DNA methylation remodeling and the functional implication during male gametogenesis in rice [0.03%]
水稻雄配子体发生过程中的DNA甲基化重编程及其功能意义
Xue Li,Bo Zhu,Yue Lu et al.
Xue Li et al.
Background: Epigenetic marks are reprogrammed during sexual reproduction. In flowering plants, DNA methylation is only partially remodeled in the gametes and the zygote. However, the timing and functional significance of ...
Bento: a toolkit for subcellular analysis of spatial transcriptomics data [0.03%]
Bento：一种用于空间转录组学数据分析的工具包
Clarence K Mah,Noorsher Ahmed,Nicole A Lopez et al.
Clarence K Mah et al.
The spatial organization of molecules in a cell is essential for their functions. While current methods focus on discerning tissue architecture, cell-cell interactions, and spatial expression patterns, they are limited to the multicellular ...
Species-aware DNA language models capture regulatory elements and their evolution [0.03%]
考虑物种的DNA语言模型捕获调控元件及其进化特性
Alexander Karollus,Johannes Hingerl,Dennis Gankin et al.
Alexander Karollus et al.
Background: The rise of large-scale multi-species genome sequencing projects promises to shed new light on how genomes encode gene regulatory instructions. To this end, new algorithms are needed that can leverage conserva...
FixNCut: single-cell genomics through reversible tissue fixation and dissociation [0.03%]
Fix-Cut：通过可逆的组织固定和消化进行单细胞基因组学研究
Laura Jiménez-Gracia,Domenica Marchese,Juan C Nieto et al.
Laura Jiménez-Gracia et al.
The use of single-cell technologies for clinical applications requires disconnecting sampling from downstream processing steps. Early sample preservation can further increase robustness and reproducibility by avoiding artifacts introduced d...
Divergent composition and transposon-silencing activity of small RNAs in mammalian oocytes [0.03%]
哺乳动物卵母细胞中小RNA的组成分化及其转座子沉默活性
Li Hou,Wei Liu,Hongdao Zhang et al.
Li Hou et al.
Background: Small RNAs are essential for germ cell development and fertilization. However, fundamental questions remain, such as the level of conservation in small RNA composition between species and whether small RNAs co...
Giulia Vittoria Ruta,Matteo Ciciani,Eyemen Kheir et al.
Giulia Vittoria Ruta et al.
Background: Further advancement of genome editing highly depends on the development of tools with higher compatibility with eukaryotes. A multitude of described Cas9s have great potential but require optimization for geno...
Next-Gen GWAS: full 2D epistatic interaction maps retrieve part of missing heritability and improve phenotypic prediction [0.03%]
下一代GWAS：全二维上位交互作用图找回部分缺失遗传力并提高表型预测能力
Clément Carré,Jean Baptiste Carluer,Christian Chaux et al.
Clément Carré et al.
The problem of missing heritability requires the consideration of genetic interactions among different loci, called epistasis. Current GWAS statistical models require years to assess the entire combinatorial epistatic space for a single phe...
Depletion of lamins B1 and B2 promotes chromatin mobility and induces differential gene expression by a mesoscale-motion-dependent mechanism [0.03%]
通过介观尺度运动依赖机制耗竭小鼠Ⅱ型核纤层蛋白促进染色质流动性并诱导差异表达基因
Emily M Pujadas Liwag,Xiaolong Wei,Nicolas Acosta et al.
Emily M Pujadas Liwag et al.
Background: B-type lamins are critical nuclear envelope proteins that interact with the three-dimensional genomic architecture. However, identifying the direct roles of B-lamins on dynamic genome organization has been cha...