Ensilication preserves high-molecular weight native DNA for clinical long-read sequencing [0.03%]
恩西利化可保护临床长读序列表达的高分子量基因组DNA
Alexis Ferrasse,Rodrigo Mendez,John E Gorzynski et al.
Alexis Ferrasse et al.
Background: Native long-read DNA sequencing simultaneously captures genetic variants and epigenetic modifications from single molecules, but preserving molecular length and base modifications currently depends on cold-cha...
Centromeric tandem repeats expansions in plateau zokor enhance chromosome stability for high-altitude adaptation [0.03%]
喜马拉雅旱獭染色体着丝点串联重复序列的扩增可增强其高原适应性
Zhen-Long Wang,Lu-Ye Shi,Xiu-Yun Liu et al.
Zhen-Long Wang et al.
Background: Repetitive elements constitute nearly 50% of mammalian genomes; however, their roles in adaptive evolution remain poorly understood. Their identification and assembly, particularly for tandem repeats, are tech...
Phylogenetic tree inference from single-cell RNA sequencing data with SCITE-RNA [0.03%]
基于单细胞RNA测序数据的SCITE-RNA谱系树重建方法
Norio Zimmermann,Xiaoyu Sun,Joanna Hård et al.
Norio Zimmermann et al.
We present SCITE-RNA, a novel phylogenetic tree inference method designed for single-cell RNA sequencing data which takes reference and alternative read counts of single-nucleotide variants as input. Our approach uses a maximum-likelihood r...
Genome and transcriptome analyses reveal parallel altitude adaptation in Chenopodium [0.03%]
基因和转录组分析揭示了草本植物属中的平行海拔适应性进化
Chaofan Zhang,Xiaolong Li,Jiangnan Huang et al.
Chaofan Zhang et al.
Background: Elucidating how crops adapt to heterogeneous environments requires integrative analyses of genome-wide variation, regulatory architecture, and evolutionary processes. Chenopodium quinoa, a globally important p...
Trans-regulation of heterochromatin underlies genetic variation in 3D genome contacts in mouse embryonic stem cells [0.03%]
在小鼠胚胎干细胞中调控异染色质的跨调节是基因组三维接触变异的基础
Haley J Fortin,Anna Z Struba,Arad Bustan et al.
Haley J Fortin et al.
Background: Genetic variation drives phenotypic diversity and disease susceptibility. Trans-acting genetic variation coordinates genome-wide chromatin changes, yet the molecular mechanisms underlying this distal regulatio...
Glycoproteomic and genetic analysis of N-glycosylation of complement component C3 reveals immune pathway regulation [0.03%]
对补体成分C3的N-糖基化进行糖蛋白组学和遗传分析以揭示免疫途径调节作用
Dinko Šoić,Najda Rudman,Oliver C Grant et al.
Dinko Šoić et al.
Background: Post-translational modifications represent a critical layer of protein regulation, yet their genetic control and population variation remain poorly characterized. Here we present a comprehensive analysis integ...
Mesopotamian ancient DNA reveals Iron Age integration of heterogeneous Bronze Age genetic ancestries following resettlement [0.03%]
美索不达米亚古代DNA揭示了在重新安置后铁器时代整合了异质性青铜时代遗传血统
Matthew P Williams,Rafał Fetner,Yassine Souilmi et al.
Matthew P Williams et al.
Background: The development of complex urban societies in Mesopotamia fundamentally shaped human history, yet the genetic dynamics underlying this process remain poorly understood. Here, we sequence DNA from 17 individual...
Benchmarking Q40 sequencing for sensitive and efficient detection of rare genomic variants [0.03%]
Q40测序表征及其在罕见基因变异敏感而高效检测中的应用
Shumeng Duan,Yaqing Liu,Xiaorou Guo et al.
Shumeng Duan et al.
Background: Phred quality score (Q score) is critical for sequencing accuracy, yet the impact of Q40-achieving sequencing technologies (99.99% accuracy) on detecting subtle biological variations remains unvalidated. ...
Premeiotic chromatin states orchestrate gene expression during male gametogenesis in rice [0.03%]
水稻配子发生过程中，次生体染色质状态调控基因表达
Bo Zhu,Feng Zhao,Qian Liu et al.
Bo Zhu et al.
Background: Male meiocyte specification from somatic tissue and male germ line development require extensive epigenetic reconfiguration. However, how the extent and the timing of the reconfiguration required for gene expr...
IDMME and IDMDE: inducible CRISPR-dCasRx platforms for spatiotemporal RNA m5C editing [0.03%]
IDMME和IDMDE：时空RNA m5C编辑的CRISPR-dCasRx平台
Jinming Xu,Jiaju Xu,Congcong Cao et al.
Jinming Xu et al.
RNA 5-methylcytosine (m5C) is a dynamic epigenetic mark implicated in tumorigenesis, however, existing editors lack spatiotemporal regulation and reversibility. Here, we develop abscisic acid (ABA)-inducible CRISPR-dCasRx systems for progra...