Genomic evidence for low genetic diversity but purging of strong deleterious variants in snow leopards [0.03%]
基因组证据表明雪豹遗传多样性较低但强烈有害变异被清除
Lin Yang,Hong Jin,Qien Yang et al.
Lin Yang et al.
Background: Long-term persistence of species with low genetic diversity is the focus of widespread attention in conservation biology. The snow leopard, Panthera uncia, is a big cat from high-alpine regions of Asia. Howeve...
Spatiotemporal transcriptomics reveals key gene regulation for grain yield and quality in wheat [0.03%]
时空转录组揭示了影响小麦产量和质量的关键基因调控机制
Xiaohui Li,Yiman Wan,Dongzhi Wang et al.
Xiaohui Li et al.
Background: Cereal grain size and quality are critical agronomic traits in crop production. Wheat grain development is governed by intricate regulatory networks that require precise spatiotemporal coordination of gene exp...
TF Profiler: a transcription factor inference method that broadly measures transcription factor activity and identifies mechanistically distinct networks [0.03%]
TFProfiler：一种广泛测量转录因子活性并识别机制上不同的网络的转录因子推理方法
Taylor Jones,Rutendo F Sigauke,Lynn Sanford et al.
Taylor Jones et al.
TF Profiler is a method of inferring transcription factor (TF) regulatory activity, i.e., when a TF is present and actively participating in the regulation of transcription, directly from nascent sequencing assays such as PRO-seq and GRO-se...
Filtering cells with high mitochondrial content depletes viable metabolically altered malignant cell populations in cancer single-cell studies [0.03%]
根据癌症单细胞研究过滤高线粒体含量的细胞可耗尽活的代谢改变的恶性细胞群
Josephine Yates,Agnieszka Kraft,Valentina Boeva
Josephine Yates
Background: Single-cell transcriptomics has transformed our understanding of cellular diversity, yet noise from technical artifacts and low-quality cells can obscure key biological signals. A common practice is filtering ...
The histone acetyltransferase CBP participates in regulating the DNA damage response through ATM after double-strand breaks [0.03%]
CBP组蛋白乙酰转移酶通过ATM参与DNA双链断裂后的损伤修复反应调节作用
Wafaa S Ramadan,Samrein B M Ahmed,Iman M Talaat et al.
Wafaa S Ramadan et al.
Background: Spatial and temporal control of DNA damage response pathways after DNA damage is crucial for maintenance of genomic stability. Ataxia telangiectasia mutated (ATM) protein plays a central role in DNA damage res...
MIDAA: deep archetypal analysis for interpretable multi-omic data integration based on biological principles [0.03%]
基于生物原理的可解释多组学数据集成的深度原型分析（MIDAA）
Salvatore Milite,Giulio Caravagna,Andrea Sottoriva
Salvatore Milite
High-throughput multi-omic molecular profiling allows the probing of biological systems at unprecedented resolution. However, integrating and interpreting high-dimensional, sparse, and noisy multimodal datasets remains challenging. Deriving...
CNRein: an evolution-aware deep reinforcement learning algorithm for single-cell DNA copy number calling [0.03%]
基于进化且适用于单细胞DNA拷贝数呼叫的深度强化学习算法CNRein
Stefan Ivanovic,Mohammed El-Kebir
Stefan Ivanovic
Low-pass single-cell DNA sequencing technologies and algorithmic advancements have enabled haplotype-specific copy number calling on thousands of cells within tumors. However, measurement uncertainty may result in spurious CNAs inconsistent...
Missing cell types in single-cell references impact deconvolution of bulk data but are detectable [0.03%]
单细胞参考中缺失的细胞类型会影响批量数据的去卷积但可以检测到
Adriana Ivich,Natalie R Davidson,Laurie Grieshober et al.
Adriana Ivich et al.
Background: Advancements in RNA sequencing have expanded our ability to study gene expression profiles of biological samples in bulk tissue and single cells. Deconvolution of bulk data with single-cell references provides...
Benchmark of cellular deconvolution methods using a multi-assay dataset from postmortem human prefrontal cortex [0.03%]
基于死后人大脑额叶皮层多模态数据的细胞去卷积方法评估研究
Louise A Huuki-Myers,Kelsey D Montgomery,Sang Ho Kwon et al.
Louise A Huuki-Myers et al.
Cellular deconvolution of bulk RNA-sequencing data using single cell/nuclei RNA-seq reference data is an important strategy for estimating cell type composition in heterogeneous tissues, such as the human brain. Here, we generate a multi-as...
Systematic optimization and prediction of cre recombinase for precise genome editing in mice [0.03%]
系统优化和预测Cre重组酶以实现小鼠的精准基因组编辑
Valerie Erhardt,Elli Hartig,Kristian Lorenzo et al.
Valerie Erhardt et al.
Background: The Cre-Lox system is a powerful tool in mouse genetics, enabling precise spatiotemporal control of gene expression and conditional knockout models. Since its development, it has transformed genome editing by ...