Cell therapy manufacturing at full clinical scale: enhancing the quality CAR-T cell therapy starting materials through massively parallel automated microfluidic cell sorting [0.03%]
全规模临床细胞治疗制造：通过大规模自动微流体分选提高CAR-T细胞疗法起始材料的质量
Alison M Skelley,Yasna Behmardi,Luke F Peterson et al.
Alison M Skelley et al.
Autologous CAR-T cell therapy has demonstrated remarkable clinical efficacy in hematologic malignancies, yet its broader application remains limited by complex, labor-intensive cell therapy manufacturing and inconsistent product quality. We...
Topology-based coordination control for multi-droplet tasks in autonomous digital microfluidics [0.03%]
基于拓扑的自主数字微流控多液滴任务协调控制方法研究
Kunlun Guo,Zerui Song,Boyi Feng et al.
Kunlun Guo et al.
Digital microfluidics (DMF) is a versatile technique for parallel and field-programmable control of individual droplets. The challenge of large-scale parallel droplet manipulation in DMF is essentially a cross-scale complex system control p...
LEGO®-inspired electrically-actuated microfluidics for on-chip protein crystallization and in situ X-ray crystallography [0.03%]
用于芯片上蛋白质结晶和原位X射线晶体学的乐高（LEGO）概念电驱动微流控设备
Sarthak Saha,Logan Chen,Gabrielle R Budziszewski et al.
Sarthak Saha et al.
X-ray crystallography has long been the workhorse technique for enabling the analysis and investigation of 3D protein structures. This understanding is crucial for deciphering protein function, including enzymatic reactions, signaling pathw...
Doyeon Lim,Youngjun Song
Doyeon Lim
This study introduces a robust selective on-chip DNA synthesis platform utilizing an electric field-assisted polymerase chain reaction (E-PCR) system based on a microelectrode array. We demonstrate the use of electric field manipulation for...
A microfluidic skin-on-a-chip enabling in situ construction of full-thickness human skin for modeling inflammatory diseases [0.03%]
一种微流体皮肤芯片，可在原位构建全厚度人体皮肤以模拟炎症性疾病
Linwei Sang,Ajing Liu,Junjie Zhu et al.
Linwei Sang et al.
Physiologically relevant in vitro skin models are crucial tools for investigating disease-related responses, evaluating drug efficacy, and enhancing the translational value of dermatological research. While skin-on-a-chip technologies enabl...
A tunable 50 MHz acoustic vortex tweezer for size-selective manipulation and cell pre-concentration [0.03%]
一种可调的50MHz声涡旋夹具用于尺寸选择性操控及细胞预富集
Xiongwei Wei,Xin Wang,Lili Miao et al.
Xiongwei Wei et al.
In microfluidic bioanalysis, efficient target manipulation, enrichment, and sample pretreatment are key bottlenecks. Although high-frequency ultrasound (>50 MHz) can provide tens of micrometers resolution and enable precise non-contact mani...
Estimating single-cell elastic modulus in a serial microfluidic cytometer from time-of-flight and fluorescence signals analysis [0.03%]
基于飞行时间和荧光信号分析的串行微流控细胞仪单细胞弹性模量估算
Graylen R Chickering,Leroy L Jia,Matthew DiSalvo et al.
Graylen R Chickering et al.
Cellular state, function, and disease all contribute to whole-cell mechanical properties. Investigating these relationships is often difficult due to low measurement throughput, inability to draw one-to-one connections between mechanical an...
Sequential intracellular delivery of genetic coding molecules using an acoustic electric microfluidic platform [0.03%]
利用声电微流控平台顺序实现细胞内遗传物质递送
Michelle Zhang,Aida Z Taravatfard,Mohammad Aghaamoo et al.
Michelle Zhang et al.
Intracellular delivery of genetic coding molecules is a critical step in both fundamental cell biology and the development of cell and gene therapies. With the emergence of diverse genetic coding molecules, next-generation cell engineering ...
Region-specific proteomic profiling of brain interstitial fluid via a micro-invasive sampling platform [0.03%]
基于微创取样的脑间质液区域性蛋白组学研究
Qun Cao,Hannah D Jackson,Aidan J Duncan et al.
Qun Cao et al.
Interstitial fluid (ISF) within the brain parenchyma contains secreted factors related to brain function, metabolism, and neurodegenerative disorders. Cerebrospinal fluid (CSF) is commonly sampled due to its accessibility in well-defined sp...
A hypoxic microfluidic organoid-on-a-chip system for studying the efficacy of metronidazole-modified nanomaterials against cholangiocarcinoma established within the chip [0.03%]
一种微流控芯片系统，用于研究甲硝唑修饰的纳米材料在芯片内建立的胆管癌中的疗效
Ao Xie,Zipeng Yao,Qijun Du et al.
Ao Xie et al.
Cholangiocarcinoma (CCA) is a highly aggressive biliary malignancy characterized by a dismal prognosis. Tumor progression relies heavily on the hypoxic tumor microenvironment (TME), a key factor that promotes drug resistance and reduces the...