High-throughput single-cell proteomics and transcriptomics from same cells with a nanoliter-scale, spin-transfer approach [0.03%]
一种纳升级离心转接方法可对同一细胞进行高通量单细胞蛋白质组学和转录组学分析
Pranav Dawar,Lye Meng Markillie,Sarah M Williams et al.
Pranav Dawar et al.
Single-cell multiomic platforms provide a comprehensive snapshot of cellular states and cell types by offering critical insights into the spatiotemporal regulation of biomolecular networks at a systems level, thereby defining the basis of m...
Gabrielle Saint-Girons,Kaustav A Gopinathan,Sajad Razavi Bazaz et al.
Gabrielle Saint-Girons et al.
Although several contact-free trapping techniques exist in microfluidics - such as optical, acoustic, and dielectrophoretic - these approaches often face tradeoffs including: limited area, fine tuning, or special buffers. Here, we introduce...
Integrated strategy for breast cancer biomarker analysis using dual ionic liquid aqueous biphasic systems and microfluidic immunoassays [0.03%]
基于双离子液体水两相体系和微流控免疫测定的乳腺癌生物标志物分析集成策略
Maria S M Mendes,Inês Agostinho,Maria C Souza et al.
Maria S M Mendes et al.
Human epidermal growth factor receptor 2 (HER2) is a key biomarker in breast cancer diagnosis, prognosis, and therapeutic decision-making. While its determination is traditionally based on tissue biopsy, serum-based testing is a simpler and...
Integrated microfluidic platform based on potentiometric Sonogel-Carbon sensors for the simultaneous determination of Na+ and K+ in untreated human plasma and serum [0.03%]
基于Potentiometric Sonogel-碳传感器的集成微流控平台同时测定人血浆和血清中的Na+和K+
Álvaro Jesús Sainz-Calvo,Álvaro Cordero-Hernández,Marina Jiménez-Rodríguez et al.
Álvaro Jesús Sainz-Calvo et al.
In the present work, two potentiometric sensors for the simultaneous determination of sodium and potassium ions were successfully developed. A novel functionalized multi-walled carbon nanotube sonogel-based mixture served as ion-to-electron...
Dissociable perfusion chip (DPC): perfusable microfluidic chip for single-cell screening of anti-cancer drugs in live glioblastoma explants [0.03%]
可分离灌注芯片（DPC）：活胶质母细胞瘤标本中单细胞抗肿瘤药物筛选的灌注型微流控芯片
Darragh G Kennedy,Wenting Zhao,Terry L Chern et al.
Darragh G Kennedy et al.
New approaches are needed to screen anti-cancer drugs that can target specific subpopulations of tumor cells. This study presents a microfluidic chip that enables parallel culture and drug perturbation of five thick tissue slices from human...
Macroporous transport - mesoporous catalysis: a rapid microfluidic-fabricated biomimetic sponge photocatalytic microsphere reactor [0.03%]
宏观输运-介观催化：一种快速微流控制造的仿生海绵光催化微球反应器
Qikai Wang,Wenwen Shi,Qihang Yang et al.
Qikai Wang et al.
To address the low light-harvesting efficiency, rapid charge recombination, and restricted mass transport in conventional photocatalysts, this study proposes a bio-inspired SiO2@TiO2 photocatalytic microsphere reactor (ST-PCMR), rapidly fab...
Real-time impedance-based cell migration measurements with integrated electrodes on porous membranes for next generation microphysiological systems [0.03%]
基于阻抗的实时细胞迁移测量集成电极在多孔膜上的用于下一代微生理系统中的细胞迁移测量
Karina Torres-Castro,Aditya Rane,Darwin R Reyes
Karina Torres-Castro
We present a novel microfluidic device capable of electrically interrogating both surfaces of a porous membrane quantitatively and in real time using electrical impedance spectroscopy to monitor cell migration. This device holds patterned g...
Xian Chen,Chuanchao Zhang,Yaping Wang et al.
Xian Chen et al.
Micro-mixing plays an important role in biochemical analyses using miniaturized total analysis systems (μTASs) or lab-on-chips. However, existing micromixers primarily rely on a specific medium and lack the capability of being activated on...
Inkube: an all-in-one solution for neuron culturing, electrophysiology, and fluidic exchange [0.03%]
Inkube：用于神经元培养、电生理和流体交换的一站式解决方案
Benedikt Maurer,Selina Fassbind,Tobias Ruff et al.
Benedikt Maurer et al.
Culturing neuronal networks in vitro is a tedious and time-consuming endeavor. In addition, how the composition of the culture medium and environmental variables such as temperature, osmolarity, and pH affect the spiking behavior of neurona...
Leyla Akh,Apresio K Fajrial,Sunwoo Sohn et al.
Leyla Akh et al.
Genetic engineering of cells has a range of applications in treating incurable diseases. Plasmid DNA is a popular choice of nucleic acid for cell engineering due to its low cost and stability. However, plasmid DNA must survive the protectiv...