From animal testing to in vitro systems: advancing standardization in microphysiological systems [0.03%]
从动物实验到体外系统：在微生理系统中推进标准化进程
Darwin R Reyes,Mandy B Esch,Lorna Ewart et al.
Darwin R Reyes et al.
Limitations with cell cultures and experimental animal-based studies have had the scientific and industrial communities searching for new approaches that can provide reliable human models for applications such as drug development, toxicolog...
Immune cells and inflammatory mediators cause endothelial dysfunction in a vascular microphysiological system [0.03%]
免疫细胞和炎症介质导致血管微生理系统中的内皮功能障碍
Aishwarya Rengarajan,Hannah E Goldblatt,David J Beebe et al.
Aishwarya Rengarajan et al.
Functional assessment of endothelium serves as an important indicator of vascular health and is compromised in vascular disorders including hypertension, atherosclerosis, and preeclampsia. Endothelial dysfunction in these cases is linked to...
Gravity-perfused airway-on-a-chip optimized for quantitative BSL-3 studies of SARS-CoV-2 infection: barrier permeability, cytokine production, immunohistochemistry, and viral load assays [0.03%]
用于SARS-CoV-2感染定量化BSL-3研究的重力灌流器官芯片系统：屏障通透性，细胞因子生成，免疫组化和病毒载量检测优化
Shannon L Faley,Niloufar A Boghdeh,David K Schaffer et al.
Shannon L Faley et al.
Human microphysiological systems, such as organs on chips, are an emerging technology for modeling human physiology in a preclinical setting to understand the mechanism of action of drugs, to evaluate the efficacy of treatment options for h...
Assessing bioartificial organ function: the 3P model framework and its validation [0.03%]
生物人工器官功能评估：“3P”模型框架及其验证
Jingmin An,Shuyu Zhang,Juan Wu et al.
Jingmin An et al.
The rapid advancement in the fabrication and culture of in vitro organs has marked a new era in biomedical research. While strides have been made in creating structurally diverse bioartificial organs, such as the liver, which serves as the ...
On-chip droplet analysis and cell spheroid screening by capillary wrapping enabled shape-adaptive ferrofluid transporters [0.03%]
通过毛细管缠绕使形状自适应磁流体运输器实现芯片上的液滴分析和细胞微球筛选
Xuejiao Wang,Xin Li,Aoyang Pu et al.
Xuejiao Wang et al.
Non-invasive droplet manipulation with no physical damage to the sample is important for the practical value of manipulation tools in multidisciplinary applications from biochemical analysis and diagnostics to cell engineering. It is a chal...
Nanoscale sorting of extracellular vesicles via optically-induced dielectrophoresis on an integrated microfluidic system [0.03%]
集成微流控系统中通过光诱导介电电泳进行纳米级外泌体分选
Wei-Jen Soong,Chih-Hung Wang,Chihchen Chen et al.
Wei-Jen Soong et al.
We reported a microfluidic system for sorting of extracellular vesicles (EVs), which can house DNAs, RNAs, lipids, proteins, and metabolites that are important in intercellular communication. Their presence within bodily fluids has demonstr...
William S Harley,Kirill Kolesnik,Daniel E Heath et al.
William S Harley et al.
Acoustofluidic micromanipulation is an important tool for biomedical research, where acoustic forces offer the ability to manipulate fluids, cells, and particles in a rapid, biocompatible, and contact-free manner. Of particular interest is ...
Integrated phase separation in microliter droplets for ultratrace-enriching biomarker analysis [0.03%]
微升液滴中的集成相分离用于超痕量生物标志物分析富集
Qihao Zha,Yong Luo,Conghui Liu et al.
Qihao Zha et al.
Ultratrace-enriching biomarker analysis is an effective method for achieving highly accurate and enhanced sensitive detection. In this study, we have developed an enrichment detection platform by combining a minipillar array with an aqueous...
Open microfluidics: droplet microarrays as next generation multiwell plates for high throughput screening [0.03%]
开放微流控：液滴微阵列作为高通量筛选的下一代多孔板
Robert Strutt,Bijing Xiong,Vanessa Fabienne Abegg et al.
Robert Strutt et al.
Multiwell plates are prominent in the biological and chemical sciences; however, they face limitations in terms of throughput and deployment in emerging bioengineering fields. Droplet microarrays, as an open microfluidic technology, organis...
A flexible electrode Array for genetic transfection of different layers of the retina by electroporation [0.03%]
用于通过电穿孔对视网膜不同层次进行基因转染的柔性电极阵列
Yu Zhang,Tao Peng,Yu Ge et al.
Yu Zhang et al.
Electroporation (in which the permeability of a cell membrane is increased transiently by exposure to an appropriate electric field) has exhibited great potential of becoming an alternative to adeno-associated virus (AAV)-based retina gene ...