Structural plasticity of green fluorescent protein to amino acid deletions and fluorescence rescue by folding-enhancing mutations [0.03%]
绿色荧光蛋白的结构塑性及其对氨基酸缺失和折叠增强突变的荧光恢复机制
Shu-su Liu,Xuan Wei,Xue Dong et al.
Shu-su Liu et al.
Background: Green fluorescent protein (GFP) and its derivative fluorescent proteins (FPs) are among the most commonly used reporter systems for studying gene expression and protein interaction in biomedical research. Most...
Kv1.3 contains an alternative C-terminal ER exit motif and is recruited into COPII vesicles by Sec24a [0.03%]
Kv1.3包含一个不同的C端内质网退出基序并由Sec24a募集到COPII囊泡中
John M Spear,Dolly Al Koborssy,Austin B Schwartz et al.
John M Spear et al.
Background: Potassium channels play a fundamental role in resetting the resting membrane potential of excitable cells. Determining the intracellular trafficking and localization mechanisms of potassium channels provides a...
Enzyme assays for synthesis and degradation of 2-5As and other 2'-5' oligonucleotides [0.03%]
关于2’-5’寡核苷酸（包括2-5A）的合成与降解的酶分析法研究
Jesper Buchhave Poulsen,Karina Hansen Kjær,Just Justesen et al.
Jesper Buchhave Poulsen et al.
Background: The 5'-triphosphorylated, 2'-5'-linked oligoadenylate polyribonucleotides (2-5As) are central to the interferon-induced antiviral 2-5A system. The 2-5As bind and activate the RNase L, an endoRNase degrading vi...
Jing Song,Ping Hong,Chengeng Liu et al.
Jing Song et al.
Background: The activity of eukaryotic DNA polymerase delta (Pol δ) plays an essential role in genome stability through its effects on DNA replication and repair. The p125 catalytic subunit of Pol δ is encoded by POLD1 ...
The androgen receptor plays a suppressive role in epithelial- mesenchymal transition of human prostate cancer stem progenitor cells [0.03%]
雄激素受体通过抑制人前列腺癌干细胞/progenitor细胞上皮间质转化发挥抑癌作用
Ma Zhifang,Wei Liang,Zhang Wei et al.
Ma Zhifang et al.
Background: To investigate the roles of androgen receptor (AR) in epithelial- mesenchymal transition (EMT) in human prostate cancer stem progenitor (S/P) cells isolated from LNCaP cell line. ...
Alternative divalent cations (Zn²⁺, Co²⁺, and Mn²⁺) are not mutagenic at conditions optimal for HIV-1 reverse transcriptase activity [0.03%]
替代二价阳离子(Zn²⁺，Co²⁺和Mn²⁺)在最佳HIV-1逆转录酶活性条件下不致突变
Vasudevan Achuthan,Jeffrey J DeStefano
Vasudevan Achuthan
Background: Fidelity of DNA polymerases can be influenced by cation co-factors. Physiologically, Mg(2+) is used as a co-factor by HIV reverse transcriptase (RT) to perform catalysis; however, alternative cations including...
Sigma-1 receptor directly interacts with Rac1-GTPase in the brain mitochondria [0.03%]
σ-1受体与脑线粒体中Rac1-GTP酶直接相互作用
Nino Natsvlishvili,Nino Goguadze,Elene Zhuravliova et al.
Nino Natsvlishvili et al.
Background: Small Rho-GTPases are critical mediators of neuronal plasticity and are involved in the pathogenesis of several psychiatric and neurological disorders. Rac-GTPase forms a multiprotein complex with upstream and...
Nickel quercetinase, a "promiscuous" metalloenzyme: metal incorporation and metal ligand substitution studies [0.03%]
一种"多功能"金属酶镍杨梅酸水解酶：有关金属参与和配体取代的研究
Dimitrios Nianios,Sven Thierbach,Lenz Steimer et al.
Dimitrios Nianios et al.
Background: Quercetinases are metal-dependent dioxygenases of the cupin superfamily. While fungal quercetinases are copper proteins, recombinant Streptomyces quercetinase (QueD) was previously described to be capable of i...
Solution structure and biophysical characterization of the multifaceted signalling effector protein growth arrest specific-1 [0.03%]
多功能信号转导分子效应蛋白生长停滞特异性基因蛋白1的溶液结构与生物物理性质表征
Katja Rosti,Adrian Goldman,Tommi Kajander
Katja Rosti
Background: The protein growth arrest specific-1 (GAS1) was discovered based on its ability to stop the cell cycle. During development it is involved in embryonic patterning, inhibits cell proliferation and mediates cell ...
The exchanged EF-hands in calmodulin and troponin C chimeras impair the Ca²⁺-induced hydrophobicity and alter the interaction with Orai1: a spectroscopic, thermodynamic and kinetic study [0.03%]
钙调蛋白和肌动蛋白C嵌合体中交换的EF手以影响Ca²⁺诱导的疏水性和与Orai1的相互作用：光谱，热力学和动力学研究
Drake Jensen,Nicole Reynolds,Ya-Ping Yang et al.
Drake Jensen et al.
Background: Calmodulin (CaM) plays an important role in Ca(2+)-dependent signal transduction. Ca(2+) binding to CaM triggers a conformational change, forming a hydrophobic patch that is important for target protein recogn...