Systematic substitutions at BLIP position 50 result in changes in binding specificity for class A β-lactamases [0.03%]
BLIP第50位系统的替代导致针对A类β-内酰胺酶结合特异性的变化
Carolyn J Adamski,Timothy Palzkill
Carolyn J Adamski
Background: The production of β-lactamases by bacteria is the most common mechanism of resistance to the widely prescribed β-lactam antibiotics. β-lactamase inhibitory protein (BLIP) competitively inhibits class A β-l...
Copper chelation and interleukin-6 proinflammatory cytokine effects on expression of different proteins involved in iron metabolism in HepG2 cell line [0.03%]
铜螯合和白细胞介素-6炎症细胞因子对肝癌HepG2细胞系中铁代谢相关不同蛋白质表达的影响
Luca Marco Di Bella,Roberto Alampi,Flavia Biundo et al.
Luca Marco Di Bella et al.
Background: In vertebrates, there is an intimate relationship between copper and iron homeostasis. Copper deficiency, which leads to a defect in ceruloplasmin enzymatic activity, has a strong effect on iron homeostasis re...
Effects of protonation on the hydrolysis of triphosphate in vacuum and the implications for catalysis by nucleotide hydrolyzing enzymes [0.03%]
质子化对真空条件下三磷酸酯水解的影响及其对核苷酸水解酶催化的启示
Farooq Ahmad Kiani,Stefan Fischer
Farooq Ahmad Kiani
Background: Nucleoside triphosphate (NTP) hydrolysis is a key reaction in biology. It involves breaking two very stable bonds (one P-O bond and one O-H bond of water), in either a concurrent or a sequential way. Here, we ...
Knockdown of GGCT inhibits cell proliferation and induces late apoptosis in human gastric cancer [0.03%]
GGCT敲低通过抑制细胞增殖并诱导晚期凋亡来发挥抑胃癌作用
Wenjie Zhang,Lei Chen,Honggang Xiang et al.
Wenjie Zhang et al.
Background: Gamma glutamylcyclotransferase (GGCT) has been proved to be involved in various cancers, but the biological function of GGCT in gastric cancer is still largely unknown. ...
Identification of replication-dependent and replication-independent linker histone complexes: Tpr specifically promotes replication-dependent linker histone stability [0.03%]
识别依赖和不依赖DNA复制的连接子组蛋白复合体：Tpr特异性促进复制依赖型连接子组蛋白稳定性
Pei Zhang,Owen E Branson,Michael A Freitas et al.
Pei Zhang et al.
Background: There are 11 variants of linker histone H1 in mammalian cells. Beyond their shared abilities to stabilize and condense chromatin, the H1 variants have been found to have non-redundant functions, the mechanisms...
Pranali Patel,Uros Kuzmanov,Seema Mital
Pranali Patel
Background: Human tyrosine-protein phosphatase non-receptor type substrate 1α (SIRPA) is a surface marker identified in cardiomyocytes differentiated from human embryonic stem cells. Our objective was to determine if cir...
Computational studies of human class V alcohol dehydrogenase - the odd sibling [0.03%]
人V类酒精脱氢酶的计算研究——这个“怪异”的家族成员
Linus J Östberg,Bengt Persson,Jan-Olov Höög
Linus J Östberg
Background: All known attempts to isolate and characterize mammalian class V alcohol dehydrogenase (class V ADH), a member of the large ADH protein family, at the protein level have failed. This indicates that the class V...
Effect of mutations to amino acid A301 and F361 in thermostability and catalytic activity of the β-galactosidase from Bacillus subtilis VTCC-DVN-12-01 [0.03%]
芽孢杆菌β-半乳糖苷酶A301和F361位点的突变对其热稳定性和催化活性的影响
Thao Thi Nguyen,Hanh Van Vu,Nhung Thi Hong Nguyen et al.
Thao Thi Nguyen et al.
Background: Beta-galactosidase (EC 3.2.1.23), a commercially important enzyme, catalyses the hydrolysis of β-1,3- and β-1,4-galactosyl bonds of polymer or oligosaccharidesas well as transglycosylation of β-galactopyran...
M Zulema Cabail,Emily I Chen,Antonius Koller et al.
M Zulema Cabail et al.
Background: Intermolecular autophosphorylation at Tyr416 is a conserved mechanism of activation among the members of the Src family of nonreceptor tyrosine kinases. Like several other tyrosine kinases, Src can catalyze th...
Sustained activation of mTORC1 in macrophages increases AMPKα-dependent autophagy to maintain cellular homeostasis [0.03%]
巨噬细胞中mTORC1持续激活通过增加AMPKα依赖性自噬来维持细胞稳态
Hongjie Pan,Xiao-Ping Zhong,Sunhee Lee
Hongjie Pan
Background: The mechanistic target of rapamycin complex 1 (mTORC1) is a well-conserved serine/threonine protein kinase that controls autophagy as well as many other processes such as protein synthesis, cell growth, and me...