Metabolic engineering of Corynebacterium glutamicum WM001 to improve l-isoleucine production [0.03%]
谷氨酸棒杆菌WM001代谢工程改进l-异亮氨酸生产
Yanchao Zhang,Yadi Liu,Shuyan Zhang et al.
Yanchao Zhang et al.
In this study, l-isoleucine production in Corynebacterium glutamicum WM001 was improved by deleting three genes in the genome, replacing the native promoter of ilvA in the genome, and overexpression of five genes in an alr-based auxotrophic...
Development of novel strategies for higher fermentative biohydrogen recovery along with novel metabolites from organic wastes: The present state of the art [0.03%]
有机废弃物产氢及新型代谢物的研究进展与发展前景
Raman Rao,Nitai Basak
Raman Rao
Depletion of fossil fuels and environmental concern has compelled us to search for alternative fuel. Hydrogen is considered as a dream fuel as it has high energy content (142 kJ g-1 ) and is not chemically bound to carbon. At present, fossi...
16S rRNA-functionalized multi-HCR concatemers in a signal amplification nanostructure for visual detection of Salmonella [0.03%]
基于信号放大的用于沙门氏菌可视化检测的16S rRNA功能化多HCR嵌合体纳米结构
Guijuan Qu,Xiuwei Sun,Na Ying et al.
Guijuan Qu et al.
To prevent foodborne diseases and minimize their impacts, it is extremely important to develop a cost-effective and efficient bacterial detection assay for diagnostics, particularly in resource-poor settings. In this study, 16S rRNA from fo...
DNA-RNA complementation on silicon wafer for thyroid cancer determination [0.03%]
基于芯片的DNA-RNA互补技术在甲状腺癌诊断中的应用研究
Subash C B Gopinath,Shijin Xuan
Subash C B Gopinath
One of the current issues with thyroid tumor is early diagnosis as it makes the higher possibility of curing. This research was focused to detect and quantify the level of specific target sequence complementation of miR-222 with capture DNA...
Purification of cell-derived Japanese encephalitis virus by dual-mode chromatography [0.03%]
利用双模式色谱法纯化细胞来源的日本脑炎病毒
Fuliang Zhang,Jun Luo,Man Teng et al.
Fuliang Zhang et al.
Purification of the enveloped virus poses a challenge as one must retain viral infectivity to preserve immunogenicity. The traditional process of virus purification is time-consuming, laborious and hard to scale up. Here, a rapid, simple an...
Computational investigations on the dynamic binding effect of molecular tweezer CLR01 toward intrinsically disordered HIV-1 Nef [0.03%]
计算研究分子钳CLR01与HIV-1 Nef内在无序蛋白的动态结合效应
Anil Bhattarai,Isaac Arnold Emerson
Anil Bhattarai
Intrinsically disordered proteins (IDPs) are highly flexible molecules that undergo disorder to order transition through their interaction with other molecules. IDPs play a vital role in several biological processes ranging from molecular r...
Application of lipase immobilized on a hydrophobic support for the synthesis of aromatic esters [0.03%]
固定在疏水性载体上的脂肪酶用于芳香族酯合成中的应用研究
Marta Maria Oliveira Dos Santos,Rafaela Santos Gama,Iasnaia Maria de Carvalho Tavares et al.
Marta Maria Oliveira Dos Santos et al.
The present study aimed at preparing three biocatalysts via physical adsorption of lipases from Candida rugosa (CRL), Mucor javanicus, and Candida sp. on a hydrophobic and mesoporous support (Diaion HP-20). These biocatalysts were later app...
Effect of phenolic compounds from the leaves of Psidium guajava on the activity of three metabolism-related enzymes [0.03%]
_psidium guajava_叶中酚类化合物对三种代谢相关酶活性的影响
Mohamed H Ahmed,Hala M Aldesouki,Farid A Badria
Mohamed H Ahmed
Enzyme activity modulation by synthetic compounds provide strategies combining the inhibitory and therapeutic mode of action of the confirmed inhibitors. However, natural modulators could offer a valuable alternative for synthetic ones for ...
Sumangala Darsandhari,Dipesh Dhakal,Biplav Shrestha et al.
Sumangala Darsandhari et al.
Tamarixetin, a monomethylated derivative of quercetin, has been reported to possess many important biological activities. In the present study, a whole cell biotransformation system was used for regiospecific methylation of quercetin to pro...
Vanillic acid as phospholipase A2 and proteases inhibitor: In vitro and computational analyses [0.03%]
香草酸作为磷脂酶A2和蛋白酶抑制剂的活性：体外和计算分析
Pedro H S Cesar,Marcus V Trento,Thais A Sales et al.
Pedro H S Cesar et al.
Enzymatic inhibition by natural compounds may represent a valuable adjuvant in snakebite serum therapy. The objective in this work was to evaluate possible in vitro interactions between vanillic acid and enzymes from Bothrops spp. and Crota...