Modulation of Ube2R1 activity by a nanobody that binds near its N-terminus [0.03%]
一个结合在Ube2R1近N端的纳米抗体对其活性的调节作用研究
Charlotte Wijne,Pavana Suresh,Richard Dela Rosa et al.
Charlotte Wijne et al.
Ube2R1 (Cdc34) is a K48-ubiquitin chain-specific ubiquitin-conjugating (E2) enzyme central to proteasomal degradation, yet the regulatory potential of its unique structural elements remains underexplored. Here, we report the isolation and b...
Huanjie Huang,Tianshu Gui,Boudewijn Mt Burgering
Huanjie Huang
The Forkhead box O (FOXO) class of transcription factors is evolutionary conserved both structurally and at least in part also functionally. FOXO activation results in transcriptional programs that provide cellular resilience toward exogeno...
ChIP happens: from biochemical origins to the modern omics toolbox for understanding steroid hormone receptors [0.03%]
从生化起源到现代组学工具箱：了解类固醇激素受体发生的染色质免疫沉淀技术
Thomas F Grimes,Jacob Pope,Jack Stenning et al.
Thomas F Grimes et al.
Nuclear steroid hormone receptors (SHRs) are ligand-activated transcription factors that mediate cellular responses to steroid hormones (SHs) through regulating gene expression. Understanding the SHR function is crucial for elucidating SH-d...
Post-translational modifications of triosephosphate isomerase reveal metabolic vulnerabilities in T-ALL. Effect of combining dichloroacetic acid and the PPI rabeprazole [0.03%]
二氯乙酸与PPI拉贝拉唑联合使用对T细胞急性淋巴细胞白血病糖酵解代谢的抑制作用及机制研究
Yoalli Martínez-Pérez PhD,Ignacio De la Mora-De la Mora PhD,Gloria Hernández-Alcántara PhD et al.
Yoalli Martínez-Pérez PhD et al.
Acute lymphoblastic leukemia, particularly the T-cell subtype, remains associated with poor outcomes in relapsed and adult patients, highlighting the need for novel therapeutic strategies. Metabolic reprogramming, especially glycolytic depe...
Zhenyu Tan,Alex Missman,Michael A Cianfrocco
Zhenyu Tan
Intracellular organization is crucial for supporting cell function in an ever-changing environment. The eukaryotic microtubule cytoskeleton and its associated motor proteins are the vast molecular highways and motor vehicles that connect, p...
Construction and regulation of microbial cell factories for enhancing the biosynthesis of O-acetyl-L-homoserine in Escherichia coli W3110 Biosynthesis of O-acetyl-L-homoserine in E. coli [0.03%]
用于增强大肠杆菌O-乙酰-L-高丝氨酸生物合成的微生物细胞工厂的构建与调控研究
Kun Niu,Yi-Fan Zhao,Zi-Xuan Zhang et al.
Kun Niu et al.
O-Acetyl-L-homoserine (OAH) is a versatile platform compound with extensive potential applications. It is a key precursor for the synthesis of L-methionine and adenosylmethionine. Currently, the microbial fermentation process for the produc...
Clostridium beijerinckii displays a soluble [FeFe]-hydrogenase/formate dehydrogenase enzyme complex that links H2 and CO2 metabolism [0.03%]
溶剂产生性产氢菌热厌氧杆菌贝杰氏亚种内源表达一种可溶性的[铁-铁]氢酶/甲醛脱氢酶复合物将H2和CO2代谢相关联
Sabrina Dezzani,Abdulrahman Alogaidi,Anca Pordea et al.
Sabrina Dezzani et al.
The production of biofuels by bacterial fermentation receives sustained attention due to the need to develop novel circular and sustainable technologies. Clostridium beijerinckii produces both hydrogen (H2) and carbon-based biofuels acetone...
Structural basis for sequence-specific DNA recognition by a group IId WRKY transcription factor GhWRKY17 in cotton [0.03%]
棉纤维素II型WRKY转录因子GhWRKY17特异性识别DNA的结构基础
Qin Xiao,Yu Wang,Xinci Shang et al.
Qin Xiao et al.
WRKY transcription factors, a plant-specific family of transcriptional regulators, are classified into four groups (I-IV) and play pivotal roles in plant defense, development, and stress responses. These proteins are characterized by conser...
Serendipitous Discovery of an Allosteric Inhibitor Binding Groove in the Proline Biosynthetic Enzyme Pyrroline-5-Carboxylate Reductase 1 (PYCR1) [0.03%]
丙氨酸生物合成酶吡咯啉-5-羧酸还原酶1(PYCR1)的变构抑制剂结合凹槽的偶然发现
Kaylen R Meeks,Caitlin J Mattingly,Jay C Nix et al.
Kaylen R Meeks et al.
Δ1-pyrroline-5-carboxylate (P5C) reductase 1 (PYCR1) catalyzes the NAD(P)H-dependent conversion of L-P5C to L-proline and is one of the most consistently upregulated metabolic enzymes in cancer cells. High PYCR1 expression is associated wi...
An expanded role for single-cell chemical genomics profiling in drug discovery [0.03%]
单细胞化学基因组学在药物发现中的作用扩大
Adeya Wyatt,Kevin Hoffer-Hawlik,Ross M Giglio et al.
Adeya Wyatt et al.
The integration of single-cell genomics into the chemical genetics paradigm is reshaping how researchers profile drug activity, prioritize lead candidates, and uncover new therapeutic opportunities. Traditional chemical genetic approaches, ...