Dual targeting of SLC6A14 and autophagy/macropinocytosis enhances therapeutic efficacy in pancreatic ductal adenocarcinoma [0.03%]
双重靶向SLC6A14和自噬/巨胞饮作用可增强胰腺导管腺癌的治疗效果
Yangzom D Bhutia,Mosharaf Mahmud Syed,Devaraja Rajasekaran et al.
Yangzom D Bhutia et al.
PDAC is highly desmoplastic and undergoes metabolic reprogramming to sustain their growth and proliferation. Our laboratory has identified SLC6A14, an amino acid transporter, as a novel drug target for PDAC. Genetic deletion of SLC6A14 or i...
Eduardo Fuentes-Lemus
Eduardo Fuentes-Lemus
Proteins are the most abundant macromolecules in biological systems. This high abundance and the presence of electron-rich side-chains make proteins a major target for biological oxidants. Protein oxidation encompasses a complex set of reac...
Sonal Garg,George-Lucian Moldovan
Sonal Garg
Single-stranded DNA gaps (ssDNA gaps) have emerged as a potential indicator of therapeutic response in cancer. Accumulation of ssDNA gaps is associated with increased sensitivity of cancer cells to genotoxic therapies like PARP inhibitors (...
The CRISPR ring nuclease Csx15 oligomerises on cyclic nucleotide binding to regulate antiviral defence [0.03%]
CRISPR 核酸酶 Csx15 通过结合环状核苷酸形成寡聚体来调控抗病毒免疫反应
Haotian Chi,Stephen A McMahon,Shirley Graham et al.
Haotian Chi et al.
Prokaryotic type III CRISPR systems signal infection by generating cyclic oligoadenylate (cOA) second messengers, which activate defence proteins allosterically, providing immunity. cOA molecules are typically degraded by extrinsic, stand-a...
Structural enzymology of a Fusarium graminearum aldehyde oxidase reveals a distinct active-site and reactivity versus its paralog galactose oxidase [0.03%]
一支顶头孢菌醛氧化酶的结构酶学揭示了其与半乳糖氧化酶同源蛋白的不同活性位点及反应性
Jessica K Fong,Laura Mazo,Alison K Nairn et al.
Jessica K Fong et al.
Copper radical oxidases (CROs), which comprise Auxiliary Activity Family 5 (AA5) in the Carbohydrate-Active Enzymes (CAZy) classification, have a long history of study due to their unique catalytic mechanism and biotechnological application...
Oligomeric assemblies of plant biotin carboxylase revealed by cryo-EM and cross-linking [0.03%]
低温电子显微镜和交联揭示了生物素羧酶的寡聚装配体
Hunter J Madison,Luke Dunn,Youngki You et al.
Hunter J Madison et al.
Due to the interest in fatty acid synthesis by oilseed crops, we conducted structural studies of the biotin carboxylase (BC) subunit of the plastid acetyl-CoA carboxylase. Acetyl-CoA carboxylase catalyzes the first committed step in the fat...
Cold exposure remodels bile acid metabolism in a mouse strain- and tissue-specific manner [0.03%]
冷暴露以小鼠品系和组织特异性的方式重塑胆汁酸代谢
Sarra Beji,Mathilde Mouchiroud,Bernie Efole et al.
Sarra Beji et al.
Cold exposure remodels bile acid metabolism, but whether these changes are conserved across mouse strains and biological compartments is unclear. We profiled bile acid composition in C57BL/6J (C57) and C3H/HeJ (C3H) mice housed at 30°C or ...
Binding of 14-3-3 stabilises recombinant AMPKγ2-containing complexes [0.03%]
14-3-3结合稳定了重组的AMPKγ2含有的复合物
David Carling,Shu-Yang Chen,Jane Bennett et al.
David Carling et al.
AMP-activated protein kinase (AMPK) plays an important role in maintaining energy homeostasis in mammals. AMPK is heterotrimer of an α catalytic subunit, and two regulatory subunits, β and γ. In mammals, each subunit has different isofor...
Structural characterization of OsPDIL2-3, a rice protein disulfide isomerase involved in prolamin accumulation [0.03%]
OsPDIL2-3的结构特征分析及其对水稻清蛋白积累的作用
Zui Fujimoto PhD,Kento Yonezawa PhD,Mutsumi Sakurai et al.
Zui Fujimoto PhD et al.
Protein disulfide isomerase-like 2-3 (OsPDIL2-3) is a rice endosperm-specific member of the PDI family that localizes to protein body-I and is essential for the accumulation of the cysteine‑rich 10-kDa prolamin crP10. OsPDIL2-3 comprises t...
Effect of ADCC-enhancing Mutations on N-glycan Profiles of Human IgG1 Antibodies [0.03%]
ADCC增强型变异对人源IgG1抗体N-聚糖谱型的影响
Lu Han,Hao Jiang,Mark Chiu
Lu Han
Human antibody-based therapeutics display characteristic N-glycan profiles that are critical determinants of developability, safety, and pharmacokinetics, and efficacy. Although Fc engineering strategies to modulate effector functions are w...