Structural and functional characterization of a metagenomically derived γ-type carbonic anhydrase and its engineering into a hyperthermostable esterase [0.03%]
γ-型碳酸酐酶的结构和功能表征及其工程化改造成为超嗜热酯酶
Charoutioun S Bodourian,Mohsin Imran,Nikolaos D Georgakis et al.
Charoutioun S Bodourian et al.
The 16S microbial community profiling of a metagenomics library from geothermal spring at Lisvori (Lesvos island, Greece) enabled the identification of a putative sequence exhibiting 95% identity to the γ-type carbonic anhydrase (γ-CA) fr...
Jimin Pei,Antonina Andreeva,Tiago Grego et al.
Jimin Pei et al.
The classification of novel protein folds remains a central challenge in structural bioinformatics, particularly as deep learning models like AlphaFold2 dramatically expand the universe of predicted protein structures. In this study, we inv...
Surface-modified protein crowders influence mutant huntingtin exon 1 aggregation via crowding effects, crowder association, and crowder solution stability [0.03%]
表面修饰的蛋白质封闭剂通过封闭效应、封闭剂缔合和封闭剂溶液稳定性影响突变型亨廷顿蛋白第1外显子聚集
Jakub Haduła,Sabrina T Krepel,Dhanya Babu et al.
Jakub Haduła et al.
Intracellular protein aggregation occurs in a highly crowded environment. The intracellular environment is highly heterogeneous, featuring diverse crowder protein surface chemistries along with varying crowder stability and solubility. It r...
Metadynamics simulations and site-directed mutagenesis determine the binding of cannabidiol to the adenosine A2A receptor [0.03%]
元动力学模拟和位点特异性诱变确定大麻二酚与腺苷A2A受体的结合
Aleix Quintana-Garcia,Iu Raïch,Claudia Del Llinas Del Torrent et al.
Aleix Quintana-Garcia et al.
Cannabidiol (CBD), the second most abundant of the active compounds found in the Cannabis sativa plant, is nonaddictive and of increasing interest due to its potential therapeutic utilities. The actions of CBD are mediated by cannabinoid re...
Role of associated water in stabilizing human γ-D crystallin under crowded eye lens conditions [0.03%]
关联水在人眼晶状体拥挤条件下稳定γ-D晶状体蛋白的作用
Bhupendra Singh,Shreya Ghosh,Akash Rana et al.
Bhupendra Singh et al.
The cytoplasm of a cell is inherently crowded with diverse biological macromolecules of varying sizes, which significantly influence thermodynamics and kinetics of essential processes such as protein folding, molecular association, and enzy...
A large-scale evolutionary and structural analysis of CLC channels and transporters [0.03%]
CLC通道和转运蛋白的大型进化和结构分析
Ayush Mishra,Gladys Díaz Vázquez,Janice L Robertson
Ayush Mishra
The CLC family of membrane proteins consists of chloride channels and anion/proton antiporters. How the same fold accommodates two distinct mechanisms remains poorly understood, and the small set of experimental structures provides limited ...
Membrane binding of a cyanobacterial ESCRT-III protein crucially involves the helix α1-3 hairpin conserved in all superfamily members [0.03%]
一个蓝藻ESCRT-III蛋白的膜结合关键依赖于所有超家族成员所保守的α1-3发夹结构中的helix α1-3
Lukas Schlösser,Mirka Kutzner,Nadja Hellmann et al.
Lukas Schlösser et al.
IM30, the inner membrane-associated protein of 30 kDa (also known as Vipp1) is essential for thylakoid membrane biogenesis and/or maintenance in chloroplasts and cyanobacteria. IM30 and its bacterial homolog PspA belong to the ESCRT-III sup...
PLNet: Persistent Laplacian neural network for protein-protein binding free energy prediction [0.03%]
持久拉普拉斯神经网络在蛋白质-蛋白质结合自由能预测中的应用
Xingjian Xu,Chunmei Wang,Guo-Wei Wei et al.
Xingjian Xu et al.
Recent advances in topology-based modeling have greatly improved molecular prediction tasks, particularly in protein-ligand binding affinity. However, when the focus shifts to predicting protein-protein interactions (PPIs) binding free ener...
Membrane-forming phospholipids allosterically modulate native-state prolyl isomerization in a CNG channel [0.03%]
膜形成磷脂以别构调节环核苷酸门控通道中的原生态脯氨酰异构化反应
Ashley J Newton,Robert D Latvala,Adefoluke E Kuforiji et al.
Ashley J Newton et al.
Ion channel activity is intricately linked to the surrounding lipid environment, yet the molecular effects of lipid-mediated regulation remain largely understudied. Here, we show that membrane-forming phospholipids, which are known to modul...