Disulfide engineering of the FANCM-MHF complex reveals constraints on crosslink design in symmetric oligomers [0.03%]
硫基工程揭示了FANCM-MHF复合物在对称寡聚体交联设计中的约束条件
Sho Ito,Tatsuya Nishino
Sho Ito
The Fanconi anemia complementation group M protein (FANCM)-MHF complex is required for branched DNA recognition in the Fanconi anemia pathway, but structural analysis of the intact complex has been hindered by dissociation of FANCM from the...
Donor-induced conformational gating and substrate-assisted catalysis in α-1,3-galactosyltransferase [0.03%]
受体诱导的构象门控及底物辅助催化作用在α-1,3-半乳糖基转移酶中的作用
Javier A Linares-Pastén,Antoni Planas
Javier A Linares-Pastén
Retaining glycosyltransferases catalyze the formation of stereochemically conserved glycosidic bonds through mechanisms that remain debated. Using bovine α1,3-galactosyltransferase (α3GalT) as a model, we combine mutagenesis, equilibrium ...
A protein chaperone can stabilize the intein-containing precursor to indirectly promote protein splicing [0.03%]
一种蛋白质分子伴侣可通过稳定内含肽前体间接促进蛋白质剪接
John S Smetana,Christopher R Powell,Noy Bagdadi et al.
John S Smetana et al.
Intervening proteins (inteins) interrupt host protein sequences and are removed by a self-mediated protein splicing reaction. Inteins are abundant in the microbial world and are often found within essential genes involved in DNA replication...
Sven Dennerlein,Peter Rehling
Sven Dennerlein
Metabolic cues regulate the formation of the mitochondrial OXPHOS machinery. These regulatory processes are tightly linked to mitochondrial translation, proteolytic degradation of unassembled subunits, and the formation of supercomplexes, c...
Pro4S: Prediction of protein solubility by fusing sequence, structure, and surface [0.03%]
基于序列、结构和表面的融合预测蛋白质可溶性(Pro4S)
Jie Qian,Lin Yang,Renxiao Wang et al.
Jie Qian et al.
Protein solubility is a critical physicochemical property influencing protein stability, therapeutic efficacy, and overall developability in drug discovery. However, traditional experimental methods for assessing solubility are often resour...
The C117D oxidation mimic reveals the monomeric structure of SARS-CoV-2 main protease [0.03%]
C117D氧化模拟体揭示了SARS-CoV-2主要蛋白酶单体结构
Sam Andress,Matthew J McLeod,Todd Holyoak
Sam Andress
The SARS-CoV-2 main protease (Mpro) is essential for viral replication and functions as a homodimer, with dimerization being critical for catalytic activity. Mpro contains an unusually high number of cysteine residues. Among these, C117 and...
Graph identification of proteins in tomograms (GRIP-Tomo) 2.0: Topologically aware classification for proteins [0.03%]
基于拓扑的蛋白质图谱鉴定软件GRIP-Tomo 2.0版本：具有拓扑感知能力的分类方法以表征电子断层扫描数据中的蛋白质分子
Chengxuan Li,August George,Reece Neff et al.
Chengxuan Li et al.
Cryo-electron tomography (cryo-ET) enables structural characterization of biomolecules under near-native conditions. Existing approaches for interpreting the resulting three-dimensional volumes are computationally expensive and have difficu...
Identification of a key water molecule involved in the macrophage migration inhibitory factor-catalyzed tautomerization of para-hydroxyphenylpyruvate using neutron crystallography [0.03%]
利用中子晶体学鉴定参与巨噬细胞迁移抑制因子催化对羟苯基乳酸互变异构的关键水分子
Gabriela C Schröder,Gregg V Crichlow,Ewelina Jablonowski et al.
Gabriela C Schröder et al.
Neutron crystallography was used to determine a 2.5-Å resolution all-atom structure of macrophage migration inhibitory factor (MIF) interacting with 3-(4-hydroxyphenyl)-pyruvate (HPP). MIF is a pro-inflammatory, pro-tumorigenic protein tha...
Quaternary structures of Streptococcus pneumoniae nucleoside diphosphate kinase: From hexamers to supramolecular assemblies [0.03%]
肺炎链球菌核苷二磷酸激酶的四聚体结构：从六聚体到超分子组装体
Julie Kerboeuf,Paul Nouri,Frédéric Galisson et al.
Julie Kerboeuf et al.
The nucleoside diphosphate kinase (NDK) is a key enzyme that controls the balance of nucleotide pools in all living organisms. Beyond this fundamental role, NDKs exert pleiotropic effects in many cellular processes, including cell developme...
The modular evolution of multiheme cytochromes c bucks the general trend observed in most proteins [0.03%]
多血红素细胞色素c的模块化进化与大多数蛋白质观察到的一般趋势相反
Ricardo Soares,Catarina M Paquete,Ricardo O Louro
Ricardo Soares
Multiheme cytochromes c are versatile enzymes which contribute to key steps in diverse biogeochemical cycles of chemical elements such as nitrogen, iron, and sulfur, and are also key players in microbial electrochemical technologies. Unders...