Human cells for human proteins: Isotope labeling in mammalian cells in suspension for functional NMR studies [0.03%]
人细胞中的人类蛋白质：悬浮哺乳动物细胞中的同位素标记用于功能性NMR研究
Philip Rößler,Marco M Ruckstuhl,Arnelle Löbbert et al.
Philip Rößler et al.
In biological and biomedical research, the focus progressively moves towards difficult human proteins, which often can only be expressed in higher eukaryotic cells. Nuclear magnetic resonance (NMR) could contribute significantly to the unde...
Supervised learning of protein variant effects across large-scale mutagenesis datasets [0.03%]
基于大规模突变数据集的蛋白质变异效应的监督学习
Thea K Schulze,Lasse M Blaabjerg,Matteo Cagiada et al.
Thea K Schulze et al.
The increasing availability of data from multiplexed assays of variant effects (MAVEs) enables supervised model training against large quantities of experimental data to learn sequence-function relationships. Variant effect scores from MAVE...
StabLyzeGraph: High-throughput screening of combinatorial mutations using graph neural networks [0.03%]
基于图神经网络的高通量组合突变筛选方法
Muhammad Waqas,Benito Natale,Michele Roggia et al.
Muhammad Waqas et al.
Engineering protein stability is a powerful strategy across biotechnology and medicine, supporting a broad range of applications such as atomic structure determination, discovery of therapeutic molecules, biomanufacturing, diagnostic reagen...
Ultrasonication-induced in vitro formation of transthyretin mature amyloid fibrils at neutral pH [0.03%]
中性pH值条件下超声诱导的转运蛋白成熟淀粉样纤维的体外形成
Keisuke Yuzu,Naoki Yamamoto,Misato Matsumura et al.
Keisuke Yuzu et al.
Transthyretin (TTR) is a plasma protein that functions as a carrier for thyroxine and retinol binding proteins. On the other hand, TTR is also known as one of the major amyloidogenic proteins, and the deposition of TTR in various organs is ...
Structural and morphological dynamics of "on-path" and "off-path" oligomers of human islet amyloid polypeptide [0.03%]
人胰岛淀粉样多肽“在途径上”和“不在途径上”的低聚物的结构和形貌动力学研究
Daniel Warren,Jadon Sitton,Dmitry Kurouski
Daniel Warren
The deposition of cytotoxic human islet amyloid polypeptide (IAPP) aggregates is a hallmark feature of Type 2 Diabetes. However, the structural evolution and cytotoxicity of IAPP aggregate species remain poorly understood. This study combin...
Vitasta Tiku,Georg Bossenz,Janine Kirstein et al.
Vitasta Tiku et al.
Mitochondrial biogenesis and functions depend on the import and assembly of more than 1000 proteins that are made as precursors on cytosolic ribosomes. The majority of these precursor proteins are transported from the ribosome to the transl...
Unveiling nuclear localization signals in human arginine deiminase proteins [0.03%]
人类鸟氨酸甲酰基转移酶核定位信号的鉴定
José L Neira,Olga Abian,Adrián Velazquez-Campoy et al.
José L Neira et al.
Arginine iminohydrolases are a family of enzymes involved in the conversion of arginine to citrulline. There are five isoforms in humans (PADI1, 2, 3, 4, and 6). Some of them are observed experimentally in the cytoplasm and in the nucleus o...
Uriel L Jean-Baptiste,Simcha R Singh,Ming J Wu et al.
Uriel L Jean-Baptiste et al.
Endothelial lipase (EL) is a key regulator of high-density lipoprotein (HDL) metabolism. Many aspects of EL function remain incompletely understood due to challenges in purifying active EL. This study identifies apolipoprotein J (ApoJ) as a...
The endoplasmic reticulum in mitochondrial protein targeting: A neuronal perspective on organelle crosstalk [0.03%]
从神经元角度探究内质网在蛋白质定位到线粒体中的作用：细胞器间的信号转导机制
J Tabitha Hees,Angelika B Harbauer
J Tabitha Hees
Neurons depend on tightly regulated spatial proteostasis to maintain function across their extended morphology. The endoplasmic reticulum (ER), traditionally known for its function in protein synthesis, folding, and trafficking, has long be...
Intermediate heterogeneity modulates coupling between chain compaction and structure formation during protein folding [0.03%]
介观不均匀性调控蛋白质折叠过程中链收缩与结构形成的耦合现象
Anushka Kaushik,Jayant B Udgaonkar
Anushka Kaushik
Polypeptide chains undergo both compaction and structure formation during folding, but the extent to which these processes are mechanistically coupled remains unclear. Although initial chain collapse can precede structure formation, the two...