Optimized ACE2-Fc fusion proteins with picomolar neutralization activity against highly evolved SARS-CoV-2 variants [0.03%]
具有纳摩尔活性的优化ACE2-Fc融合蛋白可中和高度进化的SARS-CoV-2变异毒株
Ferran Abancó,Ferran Tarrés-Freixas,Rosalba Lepore et al.
Ferran Abancó et al.
The rapid evolution of severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) has compromised the efficacy of many authorized monoclonal antibody products. This highlights the need for alternative strategies, especially for vulnerable...
Expanding the therapeutic landscape of PDE10A inhibition through alchemical absolute binding free energy calculations and in-vitro evaluation [0.03%]
基于万向绝对结合自由能计算和体外评估的PDE10A抑制剂治疗领域的拓展
Bhanu Sharma,Rahul Singh,Ashish Kumar et al.
Bhanu Sharma et al.
In preclinical models, Phosphodiesterase-10A (PDE10A) inhibition has shown efficacy for Parkinson's and Huntington's diseases and is potentially a therapeutic approach for schizophrenia. In this study, computational approaches were employed...
Mifen Chen,Soufyan Lakbir,Mihyeon Jeon et al.
Mifen Chen et al.
Until today, the exact function of mammalian odorant binding proteins (OBPs) remains a topic of debate. Although their main established function lacks direct evidence in human olfaction, OBPs are traditionally believed to act as odorant tra...
Efficient and rapid isolation of native AMPA receptor complexes for cryo-EM [0.03%]
用于冷冻电子显微镜的原生AMPA受体复合物的有效快速分离
Jumi Park,Eric Gouaux
Jumi Park
Isolating native ion channels for structural characterization is routinely achieved by extraction from membrane fractions of tissue with prolonged mild detergent treatment. AMPA receptors (AMPARs), glutamatergic receptors that mediate fast ...
Cardiomyopathy-associated and basic residue mutations in myopalladin alter actin binding, bundling, and structural stability [0.03%]
心肌病相关和基础残基变异改变肌节蛋白myopalladinn的肌动蛋白结合、聚合作用及结构稳定性
Asha Rankoth Arachchige,Julie Tran,Ziwei Zhao et al.
Asha Rankoth Arachchige et al.
Myopalladin (MYPN) is a striated muscle-specific protein essential for sarcomere integrity and actin filament organization. The Ig3 domain of MYPN mediates direct binding and bundling of filamentous actin (F-actin), and several cardiomyopat...
Guiding AlphaFold predictions with experimental knowledge to inform dynamics and interactions with VAIRO [0.03%]
利用实验知识指导AlphaFold预测以了解VAIRO的动态及相互作用
Josep Triviño,Elisabet Jiménez,Christoph Grininger et al.
Josep Triviño et al.
Structural predictions have reached unprecedented accuracy. They leverage sequence-specific data to capture all potential interactions a sequence has evolved to fulfill. AlphaFold derives information from three sources: learned parameters c...
Protein-lipid interplay governs ion channel gating and bioenergetics in human mitochondrial VDAC3 [0.03%]
蛋白质-脂质相互作用调控人源线粒体VDAC3离子通道开关及生物能量代谢功能
Aadish Rawat,Radhakrishnan Mahalakshmi
Aadish Rawat
The interaction between transmembrane proteins and their lipid environment is central to protein stability and function. Yet, the molecular factors guiding specificity of protein-lipid interactions remain poorly defined. Here, using the hum...
Stabilization versus flexibility: Detergent-dependent trade-offs in neurotensin receptor 1 GPCR ensembles [0.03%]
稳定性和灵活性之间的权衡：洗涤剂依赖性的神经紧张素受体1GPCRensemble中的trade-offs
James B Bower,Wijnand J C van der Velden,Karen P Gomez et al.
James B Bower et al.
Detergents provide essential membrane-mimetic environments for studying G protein-coupled receptors (GPCRs), but their molecular impact on receptor energetics remains incompletely understood. We combined ligand binding, thermostability meas...
The soluble state of the HIV-1 Vpu protein forms a complex with Ca2+-calmodulin [0.03%]
HIV-1病毒蛋白Vpu的可溶状态与Ca2+-钙调蛋白形成复合物
Olamide Ishola,Md Majharul Islam,Elaheh Hadadianpour et al.
Olamide Ishola et al.
The HIV-1 Vpu membrane protein is crucial to the virus lifecycle. Our recent studies revealed soluble Vpu oligomers, prompting further investigation into their interactions with cellular proteins. Notably, Vpu may form a complex with calmod...
The secreted staphylococcal biofilm protein Sbp forms biomolecular condensates in the presence of DNA [0.03%]
分泌葡萄球菌生物被膜蛋白Sbp在DNA存在下形成生物分子凝聚体
P Ethan Adkins,Alexander E Yarawsky,Andrew B Herr
P Ethan Adkins
Staphylococcus epidermidis is the leading cause of device-related infections, primarily due to its ability to form biofilms, surface-adherent bacterial communities that confer remarkable resistance to antibiotics and host defenses. Small ba...