Supplementation with the histone deacetylase inhibitor trichostatin A during in vitro culture of bovine embryos [0.03%]
组蛋白去乙酰化酶抑制剂曲古抑菌素A对体外培养牛胚胎的影响
Clara Slade Oliveira,Naiara Zoccal Saraiva,Marcela Maria de Souza et al.
Clara Slade Oliveira et al.
Trichostatin A (TSA) is a histone deacetylase inhibitor that induces histone hyperacetylation and increases gene expression levels. The aim of the present study was to establish a suitable condition for the use of TSA in in vitro cultures o...
Artificial oocyte activation and human failed-matured oocyte vitrification followed by in vitro maturation [0.03%]
人工激活卵母细胞及人未成熟卵母细胞玻璃化冷冻体外培养成熟的实验研究
Y Liu,Y X Cao,Z G Zhang et al.
Y Liu et al.
The investigation presented in this paper was conducted on the effect of oocytes activation on frozen-thawed human immature oocytes followed by in vitro maturation (IVM). A total of 386 failed-matured oocytes (germinal vesicle (GV) and meta...
Randomized Controlled Trial
Zygote (Cambridge, England). 2013 Feb;21(1):71-6. DOI:10.1017/S0967199411000530 2013
Construction of Ipr1 expression vector and development of cloned embryos in vitro [0.03%]
Ipr1表达载体的构建及体外克隆胚胎的发育研究
Yongli Song,Xiaoning He,Song Hua et al.
Yongli Song et al.
The purpose of this study was to prepare intracellular pathogen resistance 1 (Ipr1) transgenic donor cells for somatic cell nuclear transfer (SCNT). Based on our current understanding of Ipr1, a macrophage special expression vector pSP-EGFP...
P Chrenek,Z Turanová,J Slamečka Jr et al.
P Chrenek et al.
The aim of our study was to investigate the influence of vitrification on developmental rate and quality (total number of cells, number of blastomeres in inner cell mass (ICM) area, apoptotic index and embryo diameter) of transgenic (carryi...
Structural analysis of oocytes, post-fertilization events and embryonic development of the Brazilian endangered teleost Brycon insignis (Characiformes) [0.03%]
濒危鱼类巴西斑马鱼受精前后及胚胎发育的结构分析
Ziara A Isaú,Elizete Rizzo,Thiciana B Amaral et al.
Ziara A Isaú et al.
The aim of this study was to evaluate the oocytes, post-fertilization events and embryonic development in Brycon insignis, under both scanning electron microscopy and stereomicroscopy. Oocytes and embryos were sampled from spawning up to ha...
Yue-Liang Zheng
Yue-Liang Zheng
Whale oocytes recovered from follicles can be matured in vitro. Whale sperm and mature oocytes can be used for in vitro fertilization (IVF), and IVF embryos have the ability to develop to morula stage. Whale sperm injected into bovine or mo...
Effect of different culture systems on mRNA expression in developing rabbit embryos [0.03%]
不同培养体系对发育兔胚基因表达的影响
M D Saenz-de-Juano,C Naturil-Alfonso,J S Vicente et al.
M D Saenz-de-Juano et al.
The rate of zygotes in vitro developed to hatched blastocyst stage was evaluated between two different commercial media (TCM-199 and Ham's F10) and two different culture systems (renewal and non-renewal single medium) to determine the effec...
Cytochalasin B treatment of mouse oocytes during intracytoplasmic sperm injection (ICSI) increases embryo survival without impairment of development [0.03%]
卡普滴注小鼠卵母细胞进行卵浆内精子注射不损害卵裂率但可提高胚胎的存活率
Li-li Hu,Xing-hui Shen,Zhong Zheng et al.
Li-li Hu et al.
Intracytoplasmic sperm injection (ICSI) is a technique commonly used in clinical and research settings. In mouse oocytes, conventional ICSI has a poor survival rate caused by a high level of lysis. Cytochalasin B (CB) is a toxic microfilame...
Effect of lanosterol on the in vitro maturation in semi-defined culture system of prepubertal ewe oocytes [0.03%]
羊毛固醇对半确定培养体系中未成熟羊卵母细胞体外成熟的效应研究
F Marco-Jiménez,J S Vicente,M P Viudes-de-Castro
F Marco-Jiménez
The choice of medium and supplements can affect meiotic regulation and may have an impact on the regulation of mammalian oocyte growth and embryonic cell function. The aim of the present study was to assess the effects of oxygen concentrati...
The effect of growth hormone (GH) and insulin-like growth factor-I (IGF-I) on in vitro maturation of equine oocytes [0.03%]
生长激素(GH)和胰岛素样生长因子-Ⅰ(IGF-Ⅰ)对马卵母细胞体外成熟的效应
Gabriel Ribas Pereira,Pedro Luis Lorenzo,Gustavo Ferrer Carneiro et al.
Gabriel Ribas Pereira et al.
The objective of this study was to test the hypothesis that equine growth hormone (eGH), in combination with insulin growth factor-I (IGF-I), influences positively in vitro nuclear and cytoplasmic maturation of equine oocytes. Cumulus-oocyt...