Evaluation of different culture systems with low oxygen tension on the development, quality and oxidative stress-related genes of bovine embryos produced in vitro [0.03%]
不同低氧培养系统对体外生产牛胚发育、质量和氧化应激相关基因表达的影响评估
Maria Elena Arias,Raul Sanchez,Ricardo Felmer
Maria Elena Arias
The present study was conducted to assess the development, quality and gene expression profile of oxidative stress-related genes of bovine embryos cultured in different culture systems with low oxygen tension (5% CO2, 5% O2 and 90% N2). The...
Post-hatching development of bovine embryos in vitro: the effects of tunnel preparation and gender [0.03%]
雌雄对牛胚胎体外培养后发育的影响及钻孔处理的作用
Grazieli Marinheiro Machado,Ester Siqueira Caixeta,Carolina Madeira Lucci et al.
Grazieli Marinheiro Machado et al.
The objective of this study was to compare morphological characteristics, kinetics of development, and gene expression of male and female IVP embryos that were cultured until day (D)15 (fertilization = D0), using either phosphate-buffered s...
Early development of Astronotus ocellatus under stereomicroscopy and scanning electron microscopy [0.03%]
透镜和扫描电子显微镜下的豹纹燕鰩鱼早期发育过程研究
Maria do Carmo Faria Paes,Lilian Cristina Makino,Leonardo Avendaño Vasquez et al.
Maria do Carmo Faria Paes et al.
Astronotus ocellatus, popularly known as Oscar, is a cichlid fish from the Amazon basin (Brazil) with a great potential for fish farming. The aim of this research is to describe the morphology of eggs and larvae of A. ocellatus under stereo...
Leukemia inhibitory factor stimulates the transition of primordial to primary follicle and supports the goat primordial follicle viability in vitro [0.03%]
白血病抑制因子促进 goat 原始卵泡向初级卵泡的过渡并支持体外培养原始卵泡存活
Janduí Escarião da Nóbrega Jr,Paulo Bayard Dias Gonçalves,Roberta Nogueira Chaves et al.
Janduí Escarião da Nóbrega Jr et al.
The aim of this study was to evaluate the effect of leukemia inhibitory factor (LIF) on the activation and survival of preantral follicles cultured in vitro enclosed in ovarian fragments (in situ). Goat ovarian cortex was divided into fragm...
Effect of serum on the mitochondrial active area on developmental days 1 to 4 in in vitro-produced bovine embryos [0.03%]
胎盘血清对体外生产胚胎发育第1至第4天线粒体活性面积的影响
M Crocco,R H Alberio,L Lauria et al.
M Crocco et al.
Certain morphological changes at the subcellular level caused by the current techniques for in vitro embryo production seem to affect mitochondria. Many of these, including dysfunctional changes, have been associated with the presence of se...
Participation of the 39-kDa glycoprotein (gp39) of the vitelline envelope of Bufo arenarum eggs in sperm-egg interaction [0.03%]
阿根廷地蛙卵 envelopes39kDa糖蛋白(gp39)参与精子与卵的相互作用
Daniel Barrera,Ricardo J Llanos,Dora C Miceli
Daniel Barrera
The acquisition of egg fertilizability in Bufo arenarum takes place during the oviductal transit and during this process the extracellular coelomic envelope (CE) of the eggs is converted into the vitelline envelope (VE). It has been stated ...
Effects of different cryopreservation methods on post-thaw culture conditions of in vitro produced bovine embryos [0.03%]
不同冷冻方法对体外生产牛胚胎解冻后培养条件的影响
Alessandra Corallo Nicacio,Renata Simões,Fabiola Freitas de Paula-Lopes et al.
Alessandra Corallo Nicacio et al.
The aim of this work was to evaluate the effect of cryopreservation protocols on subsequent development of in vitro produced bovine embryos under different culture conditions. Expanded in vitro produced blastocysts (n = 600) harvested on da...
Expression of ZO-1 and occludin at mRNA and protein level during preimplantation development of the pig parthenogenetic diploids [0.03%]
猪孤雌发育期ZO-1和occludin的mRNA及蛋白表达模式研究
Shangdan Xu,Jibak Lee,Masashi Miyake
Shangdan Xu
Expression of mRNAs and proteins of ZO-1 and occludin was analyzed in pig oocytes and parthenogenetic diploid embryos during preimplantation development using real-time RT-PCR, western blotting and immunocytochemistry. All germinal vesicle ...
Ultrastructural changes of sheep cumulus-oocyte complexes following different methods of vitrification [0.03%]
不同玻璃化方法对绵羊卵母细胞-卵丘复合体超微结构的影响
Bita Ebrahimi,Mojtaba Rezazadeh Valojerdi,Poopak Eftekhari-Yazdi et al.
Bita Ebrahimi et al.
To determine the ultrastructural changes of sheep cumulus-oocyte complexes (COCs) following different methods of vitrification, good quality isolated COCs (GV stage) were randomly divided into the non-vitrified control, conventional straw, ...
Okadaic acid-sensitive phosphatase is related to MII/G1 transition in mouse oocytes [0.03%]
小鼠卵母细胞MII/G1转换与okadaic acid敏感性磷酸酶相关
Naoko Moride,Akira Kuwahara,Ayako Sutoh et al.
Naoko Moride et al.
It is reported that okadaic acid (OA)-sensitive phosphatase is related to mitogen-activated protein kinase (MAPK)/p90rsk activation in mammalian oocytes. OA is also involved in the positive feedback loop between M phase-promoting factor (MP...