NAD+ supply and redox state limit developmental speed in the Drosophila eye [0.03%]
果蝇眼睛中的烟酰胺腺嘌呤二核苷酸供应和氧化还原状态限制了发育速度
Nisha Veits,Yuting Guo,Jingjing He et al.
Nisha Veits et al.
The cellular and biochemical processes that define the speed at which embryos develop, tissues form, and cells differentiate remain largely unknown. Using the speed of progression of a differentiation front in the developing Drosophila eye ...
One-step generation of T-cell receptor knock-in mice in the TCRβ locus [0.03%]
一次性在TCRβ位点生成T细胞受体敲入小鼠
Jana Bilanovic,Juliana Bortolatto,Susu Duan et al.
Jana Bilanovic et al.
Transgenic mouse models expressing predefined T-cell receptors (TCRs) have been instrumental in advancing our understanding of T-cell biology. However, these traditional models rely on random genomic insertion of large constructs, require l...
Host-transposable element coexistence: a matter of resistance, tolerance and trade-off [0.03%]
宿主与转座子的共存：抵抗、忍耐和权衡的问题
Séverine Chambeyron,Alain Pélisson,Yuica Koga et al.
Séverine Chambeyron et al.
Transposable elements (TEs) are ubiquitous, mobile DNA elements that often exist as multiple copies within host genomes. To persist despite ongoing mutational decay, these genomic parasites must continuously generate new insertions into the...
A CHK1-mediated phosphorylation switch suppresses human Topoisomerase 1-associated genomic instability [0.03%]
CHK1介导的磷酸化调控开关抑制人拓扑异构酶1相关基因组不稳定性
Ananda Guha Majumdar,Nitish Chauhan,Pooja Gupta et al.
Ananda Guha Majumdar et al.
Topoisomerase 1 (TOP1) is essential for relieving DNA supercoils during replication and transcription. However, its transient reaction intermediates (TOP1 cleavage complexes or TOP1-DNA covalent complexes, i.e., TOP1ccs) become highly genot...
Short autoinhibitory sequences control phase separation of an essential bacterial transcription termination factor [0.03%]
短自抑制序列控制基本细菌转录终止因子的相分离
Emilia Krypotou,Kiersten M Ruff,Leah K McKinney et al.
Emilia Krypotou et al.
Within cells, across diverse organisms, macromolecular condensation enables spatial and temporal organization of biochemical reactions by organizing proteins and nucleic acids into compositionally distinct membraneless biomolecular condensa...
Benchmarking plant single cell RNA-sequencing sample processing strategies [0.03%]
植物单细胞RNA测序样本处理策略的基准测试
Thomas Eekhout,Lindsy De Veirman,Jolien De Block et al.
Thomas Eekhout et al.
The isolation of single plant cells from complex tissues is prone to selective enrichment and sampling biases, which complicates accurate profiling of the large diversity in cell types. Optimizing methodologies for cell enrichment and singl...
Beyond quality control: biological roles of nonsense-mediated RNA decay [0.03%]
超越质量控制：无意义介导的RNA降解的生物学作用
Kun Tan,Miles F Wilkinson
Kun Tan
Nonsense-mediated RNA decay (NMD) was originally discovered by virtue of its "quality control" function of degrading aberrant mRNAs with premature termination codons (PTCs). NMD was subsequently found to be a highly selective and conserved ...
Structural insights into bacterial dimethylsulfoniopropionate import by BCCT-family transporters [0.03%]
BCCT家族转运蛋白介导细菌对二甲基硫代丙酸内盐摄取的结构洞察
Yu-Zhong Zhang,Wen-Jing Zhu,Kang Li et al.
Yu-Zhong Zhang et al.
Dimethylsulfoniopropionate (DMSP) is a ubiquitous marine organosulfur compound central to microbial stress responses, chemotaxis, and nutrient cycling. Its catabolism produces dimethylsulfide (DMS), a climate-active gas, and plays a key rol...
Jessica Spindler,Francesca Pandolfo,Anna Eva Koch et al.
Jessica Spindler et al.
Faithful DNA replication is essential for genome stability, yet replication forks face constant stress. The Bloom syndrome helicase (BLM) safeguards fork integrity, but excessive BLM activity can itself induce replication stress. We identif...
Cytosolic DNA inhibits rDNA transcription by retaining the RNA polymerase I transcription machinery [0.03%]
细胞质DNA通过滞留RNA聚合酶I转录装置抑制rDNA转录
Yinfeng Xu,Qian Wang,Chuying Qian et al.
Yinfeng Xu et al.
Cytosolic DNA, derived from cellular damage or microbial infection, functions as a pivotal trigger for the host innate immune responses by activating intracellular DNA-sensing machinery, including the cGAS-STING pathway. However, whether cy...