Efficient CRISPR/Cas9-mediated knockin of reporter genes in rats at ROSA26 by pronuclear microinjection [0.03%]
高效ROSALIND-CRISPR-CAS9介导的报道基因敲入大鼠的方法
Takaya Abe,Ken-Ichi Inoue,Hiroshi Kiyonari
Takaya Abe
The genetic modification of rats is a key technology for advancing biomedical research on human diseases. CRISPR/Cas9-mediated genome editing enables the generation of knockout rats in a single step, without the need for embryonic stem cell...
Integrative approaches in Xenopus-based research: A report from the 1st Asian Xenopus Conference [0.03%]
基于非洲爪蟾研究的整合方法：来自第一届亚洲非洲爪蟾会议的报告
Dong Gil Jang,Hyo Jung Sim,Keun Yeong Kwon
Dong Gil Jang
The first Asian community conference on "Scientific research using Xenopus as an animal model system" was held in Osaka, Japan, from November 24 to 26, 2024. This event was organized by the Korean Society for Xenopus Development of KSMCB (K...
Tadashi Nomura,Chiaki Ohtaka-Maruyama
Tadashi Nomura
The mammalian neocortex is characterized by tangential surface expansion and a six-layer layered structure. However, developmental mechanisms underlying the evolution of the neocortex remain to be elucidated. We hold the symposium entitled ...
Chisaki Shima,Ayaka Ohashi,Saya Furukawa et al.
Chisaki Shima et al.
As skin ages, its structure and function undergo significant transformations driven by complex cellular and molecular processes. In this study, we explore these changes using the axolotl, an amphibian model known for its transparent skin, a...
Atsuo Kawahara,Sakyo Yasojima,Junko Koiwa et al.
Atsuo Kawahara et al.
The adap1 (ADP-ribosylation factor GTPase-activating protein [ArfGAP] with dual pleckstrin homology [PH] domains 1) gene is predominantly expressed in the mouse brain and is important in neural differentiation and development. However, the ...
Transforming growth factor-β-mediated regulation of atoh1-expressing neural progenitors is involved in the generation of cerebellar granule cells in larval and adult zebrafish [0.03%]
转化生长因子-β介导的atoh1表达的神经前体细胞调控参与斑马鱼幼鱼和成年小脑颗粒细胞的生成
Jui Chun Wang,Takashi Shimizu,Masahiko Hibi
Jui Chun Wang
Granule cells in the cerebellum are the most numerous neurons in the vertebrate brain. They are derived from neural progenitor cells that express the proneural gene atoh1 (atoh1a, b, c in zebrafish) during early neurogenesis. In zebrafish, ...
The property of larval cells of the scleractinian coral, Acropora tenuis, deduced from in vitro cultured cells [0.03%]
从群体外培养细胞推断的鹿角珊瑚Acropora tenuis幼虫细胞的特性
Kaz Kawamura,Satoko Sekida,Koki Nishitsuji et al.
Kaz Kawamura et al.
In previous studies, we have established approximately 15 cultured cell-lines derived from planula larvae of Acropora tenuis. Based on their morphology and behavior, these cells were classified into three types, flattened amorphous cells (F...
Chromosomal localization of PHOX2B during M-phase is disrupted in disease-associated mutants [0.03%]
与疾病相关的PHOX2B变异会破坏其在细胞分裂期的染色体定位
Yuki Sato,Shinichi Hayashi,Souichi Oe et al.
Yuki Sato et al.
In the M-phase, the nuclear membrane is broken down, nucleosomes are condensed as mitotic chromosomes, and transcription factors are generally known to be dislocated from their recognition sequences and dispersed to the cytoplasm. However, ...
Mild cryoinjury in zebrafish fin induces regenerative response without blastema formation [0.03%]
轻度冷冻损伤诱导斑马鱼鳍再生响应但不形成 blastema
Takafumi Yoshida,Atsushi Kawakami
Takafumi Yoshida
Previous studies have shown that tissue regeneration induces expression of genes that play important roles in regeneration. Recently, several studies have identified regeneration-response enhancers (RREs) that activate gene expression by ti...
Understanding disorders of the human nervous system: How fish models reveal disease mechanisms from single molecules to behavior (part 2) [0.03%]
解析人类神经系统紊乱：如何通过鱼类模型揭示从单一分子到行为的致病机制（二）
Christina Lillesaar,William Norton,Daniel Liedtke et al.
Christina Lillesaar et al.
The usefulness of zebrafish for understanding the human nervous system is exemplified by the articles in part 1. The virtual special issue part 2 not only covers more work using this well-established species, but also highlights that other ...