Integrating the Biosynthesis and Genetic Encoding of Noncanonical Amino Acids for Enzyme Design and Catalysis [0.03%]
非 canonical 氨基酸的生物合成和遗传编码整合在酶设计及催化中的应用
Yuhui Sheng,Zhi Zhou
Yuhui Sheng
Artificial enzymes engineered by site-specific incorporation of catalytically active noncanonical amino acids (ncAAs) into protein scaffolds represent a rapidly advancing class of biocatalysts, particularly for chemical transformations lack...
Activated Carbon-Supported Nanoscale Zero-Valent Iron for CO2 Biomethanation: Stability, Transport in Porous Media, and Potential Reaction Pathways [0.03%]
活化碳负载纳米零价铁用于二氧化碳生物甲烷化：稳定性、多孔介质中运移特性及潜在反应路径
Zhan-Hong Chen,Lei Zhou,Yu-Xuan Li et al.
Zhan-Hong Chen et al.
Nanoscale zero-valent iron (nZVI) is an effective electron donor for the microbial reduction of CO2 to methane as an energy carrier. Nevertheless, the natural aggregation of nanostructures in aqueous porous media such as oil reservoirs is s...
Dissecting the Chemical and Thermal Stabilities of Tetrads in G-Quadruplexes to Derive a Structure-Activity Relation for a Thrombin-Binding DNA G-Quadruplex Aptamer [0.03%]
通过分析G-四链体中四联体的化学和热稳定性来推导凝血酶结合DNA G-四链体适配子的结构活性关系
Julia Wirmer-Bartoschek,Jan-Peter Ferner,Alexander Heckel et al.
Julia Wirmer-Bartoschek et al.
Guanosine- and deoxyguanosine-rich nucleic acids can form G-quadruplex structures (G4) that are stabilized by guanine tetrads (G4 tetrads). G4s find numerous applications in biotechnology. Here, we study a so called thrombin-binding aptamer...
Specific and Multi-Product Clade I and Clade IV Sesquiterpene Synthases Contribute to the Psilocybe cubensis Volatilome [0.03%]
石硫醚类I族和IV族倍半萜合成酶对Psilocybe cubensis挥发性代谢组的贡献
Sebastian Schober,Lisa Dorfmann,Karl Walther et al.
Sebastian Schober et al.
Apart from the psychedelic psilocybin, the metabolite spectrum of Psilocybe "magic mushrooms" comprises sesquiterpenes, a class of natural products known to exhibit receptor-modulating bioactivities. However, the composition of the sesquite...
Synthetic Cell-Based Artificial Stem Cell Niches for Hematopoietic Stem Cell Differentiation [0.03%]
基于合成细胞的造血干细胞分化人工生态位
Ivaylo Balabanov,Sara Madureira,Anna Burgstaller et al.
Ivaylo Balabanov et al.
Hematopoietic stem cells (HSCs) receive a combination of biochemical and biomechanical signals within the bone marrow that guide their differentiation process. These include soluble factor signaling with cytokines, cellular confinement in t...
Enzyme-Coupled Fluorescence Assays for the Detection and Quantification of Amino Acids [0.03%]
酶偶联荧光测定法在氨基酸检测和定量中的应用
Jhilik Mondal,Afzal Azim,Syed Masood Husain
Jhilik Mondal
Amino acids are fundamental to cellular physiology, and deviations in their concentrations are closely linked with metabolic and pathological disorders. Accurate and rapid quantification of amino acids is therefore essential for clinical di...
Postsynthetic Construction and Properties of Oligonucleotides Containing Fluorescent 7-Arylethenyl-7-Deazaguanosine Derivatives [0.03%]
含荧光7-芳亚乙烯-7-脱氮鸟苷衍生物的寡核苷酸的合成后修饰及其性质研究
Miho Kawaguchi,Yasufumi Fuchi,Yuta Ito et al.
Miho Kawaguchi et al.
Fluorescent nucleobase analogs (FBAs) are widely used probes for nucleic acid analysis. In this study, we designed, synthesized, and investigated fluorescent 7-arylethenyl-7-deazaguanosine derivatives as novel FBAs conjugated to molecular r...
Heterologous Production of Barnesin A, an NRPS-PKS Hybrid Containing a Rare Vinylogous Arginine Moiety [0.03%]
异源生产巴恩辛A及其罕见的烯丙基精氨酸结构单元
Sven Balluff,Marie Dayras,Christine Beemelmanns
Sven Balluff
Natural products containing vinylogous amino acids are rarely found in nature and often possess significant biological activity. Barnesin A was the first NP reported from an anaerobic bacterium (Sulfurospirillum barnesii) postulated to be b...
Biocatalytic Indigo Synthesis From L-Tryptophan Using a Three-Step Cascade Without Cofactor Regeneration [0.03%]
一种无需辅因子再生的L-色氨酸生物催化法合成靛蓝的方法
Vivian P Willers,Nikola Lončar,Marco W Fraaije
Vivian P Willers
Indigo is currently produced from petrochemical sources, which poses significant environmental challenges. Sustainable biotechnological alternatives are therefore highly desirable. Enzymatic synthesis of indigo from L-tryptophan via indole ...
Luminescent Fe3O4 Nanohybrid for Intra-Cellular Imaging and Combinatorial Chemo-Photothermal Therapy in Cancer [0.03%]
用于细胞内成像及癌症综合化疗和光热治疗的发光Fe3O4纳米复合材料
Bijaideep Dutta,Sonali Gupta,Sharanaya Purandare et al.
Bijaideep Dutta et al.
Highly stable water dispersible carbon dot decorated hybrid magnetic nanoparticles (FCDs) that combines magnetic Fe3O4 nanocrystals and fluorescent carbon dots (GUCDs) were successfully synthesized using a facile strategy. The resultant Fe3...