Sustainable Biocatalytic Synthesis of Chiral Cyanohydrins Using Engineered Hydroxynitrile Lyases [0.03%]
利用工程化羟腈酶的可持续生物催化合成手性氰醇
Mohit,Sowmya Andole,Shalini Mishra et al.
Mohit et al.
Enantiopure cyanohydrins are versatile chiral intermediates of several bioactive molecules and life-saving drugs. Consequently, the demand for their efficient and sustainable preparation has grown significantly. We aimed to synthesise them ...
Molecular Dynamics Study of the Binding of Cationic, Anionic, and Neutral Luminescent Conjugated Ligands to the Alzheimer Folds of Aβ(1-42) and Tau Fibrils [0.03%]
阳离子、阴离子和中性发光共轭配体与Aβ(1-42)淀粉样原纤维和Tau原纤维结合的分子动力学研究
Yogesh Todarwal,Mathieu Linares,Patrick Norman
Yogesh Todarwal
Unbiased atomistic molecular dynamics simulations have been employed to examine the binding interactions between amyloid fibrils (Aβ(1-42) and tau) with various ligands: anionic pFTAA, qFTAA-CN, neutral HS-276, and cationic bTVBT4. The lig...
Lei Liu,Peiyong Song,Meiqi Luo et al.
Lei Liu et al.
Nature has evolved sophisticated light-harvesting systems and sensory mechanisms that detect light, convert it into biochemical energy, regulate biological processes, and synthesize organic compounds and oxygen. Extensive studies have explo...
Mechanistic Insight into Conformational Control of Enzyme Activity by Genetically Encoded Metal-Responsive Switches [0.03%]
基因编码金属响应开关控制酶活性的构象机制研究
Payal,Jonathan Thirman,Katherine A Edmonds et al.
Payal et al.
We previously introduced a genetically encoded, metal-responsive system for reversible control of protein function based on metal chelation by bipyridylalanine (BpyAla) residues. The efficacy of this linking group approach was demonstrated ...
How to Enhance the Applicability of Metal Ion Affinity-bound Biocatalysts? Comprehensive Overview and Case Study With a Phenylalanine Ammonia Lyase and an Amine Transaminase [0.03%]
如何增强金属离子亲和结合生物催化剂的适用性？苯丙氨酸氨酶和胺转氨酶的综合概述与案例研究
Máté Laurinyecz,Bálint Alács,Zsófia Molnár et al.
Máté Laurinyecz et al.
Selective metal ion affinity binding as a simple and renewable enzyme immobilization was investigated using the same affinity function on various supports. Phenylalanine ammonia-lyase from parsley (PcPAL) and an amine transaminase from Vibr...
Mapping the SHP2 Allosteric Pocket With Target-Biased Covalent Fragments [0.03%]
基于靶点的SHP2 allostericpocket共价片段映射研究
Nina-Louisa Efrém,Noémi Csorba,Machoud Amoussa et al.
Nina-Louisa Efrém et al.
Targeted covalent inhibitors (TCIs) form covalent bonds with a specific amino acid in their target proteins, offering high selectivity and sustained pharmacologic effects. However, identifying optimal electrophilic warheads and nucleophilic...
Juliane Breiltgens,Ziruo Zou,Sascha Ferlaino et al.
Juliane Breiltgens et al.
S-adenosyl-l-methionine (SAM)-dependent methyltransferases (MTs) are generally classified as C-, O-, N-, S-, or halide MTs depending on their methyl acceptor. C-MTs catalyze selective methylation reactions of carbon nucleophiles and play a ...
Single-Molecule Analysis of Conformation Dynamics and Transition Kinetics of DNA Holliday Junction by Single-Molecule Fluorescence Resonance Energy Transfer [0.03%]
单分子荧光共振能量转移法分析DNA Holiday夹字的构象动态与转换动力学
Pratibha Agarwala,Saikat Sadhukhan,Soumen Mondal et al.
Pratibha Agarwala et al.
The relationship between conformational dynamics and multistate transition kinetics has been the fundamental focus for understanding the DNA Holliday junction (HJ) intermediated homologous genetic recombination process. Although the crystal...
Fluorogenic Peptide Aptamer Generated Using Bioorthogonal cDNA Display Technology for Cellular Imaging of Heat Shock Protein 90α [0.03%]
利用生物正交cDNA展示技术生成的用于热休克蛋白90α细胞成像的荧光肽适配体
Takanori Uzawa,Seiichi Tada,Chinmay Phadke et al.
Takanori Uzawa et al.
Fluorogenic probes are valuable tools for rapid detection and bioimaging of target molecules without requiring bound/free separation. Although antibody-based probes are widely used, their large size, reliance on secondary labeling, and limi...
Harnessing S. aureus tRNA Guanine Transglycosylase for Efficient Site-Specific Covalent Labeling of DNA [0.03%]
利用金黄色葡萄球菌tRNA鸟嘌呤转糖基酶对DNA进行高效定点共价标记
Zhenglong Zhai,Evan McCormack,Alexander Harjung et al.
Zhenglong Zhai et al.
Site-specific DNA modification enables the construction of functional nucleic acid architectures for imaging, diagnostics, and information encoding. Bacterial tRNA guanine transglycosylases (TGTs) have been shown to exchange guanine-34 in c...