Super-Resolution Live-Cell Mapping of Protein-Protein Interactions Using Chemogenetic Split Reporters and Stimulated Emission Depletion Microscopy [0.03%]
基于化学遗传学拆分报告基因和受激发射损耗显微镜的蛋白质相互作用超分辨活细胞图谱技术
Stephanie Board,Arnaud Gautier
Stephanie Board
The ability to map protein-protein interactions (PPI) within living cells at high spatial resolution is essential for unraveling their roles in cellular biology. Although several super-resolution microscopy techniques are available, visuali...
Practical Tips for Intentional and Inclusive Recruiting and Hiring for Academic Research Laboratories [0.03%]
学术研究实验室有意图和包容性招聘的实用建议
Madison K C Seto,Taiasean Wu,Aaron M Leconte
Madison K C Seto
Hiring decisions are some of the most important choices PIs make for their research groups. Whether it is undergraduates, graduate students, postdoctoral researchers, or other research assistants, trainees are pivotal for the success of a r...
Multifaceted Effects of a Multiple Nitric Oxide Photoreleaser and its Photoproducts on Amyloid-β Aggregation [0.03%]
多硝基氧化物光释放剂及其光产物对淀粉样蛋白聚集的多重影响
Francesca Laneri,Cristina Parisi,Salvatore Sortino
Francesca Laneri
Aberrant aggregation of β-amyloid (Aβ) peptides into insoluble fibrils is recognized as one of the hallmarks of Alzheimer's disease (AD). Among the post-translational modifications influencing Aβ behavior, nitration and nitrosation by ni...
Dynamic Anchoring of Peptides to the Extracellular Matrix Enabled by Boronic Acid [0.03%]
肽动态锚定至细胞外基质的硼酸促进法
Martin Mergenthaler,Eduardo Merino Asumendi,Andreas Inho Höpfel et al.
Martin Mergenthaler et al.
Given the extracellular matrix (ECM) essential role in tissue development, maintenance, and repair, exploiting its glycan structures offers new opportunities for dynamic drug storage and targeted delivery of peptides via injectable solution...
Christina Möller,Fabian Pohlschröder,Jan Wesche et al.
Christina Möller et al.
The transfusion of blood products requires ABO compatibility due to A and B antigens on blood cells and their corresponding antibodies in plasma. The supply of red cell concentrates (RCCs) and platelet concentrates (PCs) is challenged by ri...
Discovery and Tuning of RNA Editing Guides via High-Throughput Screening and Chemical Modification [0.03%]
基于高通量筛选及化学修饰的RNA编辑引导序列的挖掘与优化
Prince J Salvador,Sherry Lin,Megan M Chinn et al.
Prince J Salvador et al.
Adenosine deaminases acting on RNA (ADAR) catalyze the deamination of adenosine to inosine in double-stranded RNA. Because inosine is read as guanosine during translation, this process enables programmable A-to-G recoding at the transcript ...
Development of Granzyme a Turn-ON Fluorescent Activity-Based Probes [0.03%]
Granzyme A“开亮”型荧光活性探针的开发
Sebastian M Malespini,Muhammad Kazim,Euna Yoo
Sebastian M Malespini
Granzyme A (GzmA), a serine protease highly expressed in cytotoxic immune cells, plays complex roles in antitumor immunity and inflammation. While elevated GzmA levels correlate with favorable outcomes in certain cancers, extracellular GzmA...
Tong Ye Wang,Parmeetpal Dhillon,Svetlana M Krylova et al.
Tong Ye Wang et al.
The Michaelis constant (Km) is central to enzyme kinetics, guiding variant selection, inhibitor screening, and metabolic modeling. However, Km obtained by nonlinear regression can be substantially inaccurate even when the reported standard ...
Atanasio Gómez-Mulas,Elisabeth Schaffner-Reckinger,Hanne Peeters et al.
Atanasio Gómez-Mulas et al.
PDE6D is a trafficking chaperone of prenylated proteins, such as small GTPases. Several small molecule inhibitors have been developed against it, given that the oncogene K-Ras is one of the cargo proteins. Inhibitor development suffers from...
DNA-Based Ultrasensitive Cu(II) Sensor: Troponyl-Thymidine Oligo (T*4T) Forms Copper-Mediated Duplex Structure [0.03%]
基于DNA的超高灵敏度Cu(II)传感器：Troponyl-thymidine寡聚体(T*4T)形成铜介导的双链结构
Ankita Panda,Amarnath Bollu,Avani A et al.
Ankita Panda et al.
DNA-metal complexes provide new dimensions in synthesizing DNA-based conducting biomaterials such as aptamers, DNAzymes, and their metal base pairing duplex structure. Structural modifications in the native DNA generate metal-specific bindi...