In vivo Labeling and Intravital Imaging of Bacterial Infection using a Near-infrared Fluorescent D-Amino Acid Probe [0.03%]
近红外荧光D-氨基酸探针活体标记和细菌感染的在体成像
Yixuan Li,Yingjun Zhou,Yahui Du et al.
Yixuan Li et al.
Bacterial infections still pose a severe threat to public health, necessitating novel tools for real-time analysis of microbial behaviors in living organisms. While genetically engineered strains with fluorescent or luminescent reporters ar...
Bettina M Nestl,Bernd A Nebel,Verena Resch et al.
Bettina M Nestl et al.
Recent advances in bioeconomy allow a holistic view of existing and new process chains and enable novel production routines continuously advanced by academia and industry. All this progress benefits from a growing number of prediction tools...
Chemical Production of Cytotoxic Bispecific Antibodies Using the Ugi Multicomponent Reaction [0.03%]
基于Ugi多组分反应生产细胞毒双特异性抗体
Valentine Vaur,Ilias Koutsopetras,Stéphane Erb et al.
Valentine Vaur et al.
Bispecific antibodies (bsAbs) have recently emerged as a promising platform for the treatment of several conditions, most importantly cancer. Based on the combination of two different antigen-binding motifs in a single macromolecule; bsAbs ...
Nuriye Korkmaz,Muhammad Usman,Minyoung Kim
Nuriye Korkmaz
C-terminal truncated variants (A, VA, NVA, ANVA, FANVA and GFANVA) of our recently identified Cu(II) specific peptide "HGFANVA" were displayed on filamentous fd phages. Wild type fd-tet and engineered virus variants were treated with 100 mM...
Inhibition of RNA Phosphodiester Backbone Cleavage in the Presence of Organic Cations of Different Sizes [0.03%]
不同大小的有机阳离子存在下抑制RNA磷酯主链切割
Senri Yoshioka,Asuka Doi,Shu-Ichi Nakano
Senri Yoshioka
Living cells contain various types of organic cations that may interact with nucleic acids. In order to understand the nucleic acid-binding properties of organic cations of different sizes, we investigated the ability of simple organic cati...
Amplification- and Enzyme-Free Magnetic Diagnostics Circuit for Whole-Genome Detection of SARS-CoV-2 RNA [0.03%]
无信号放大和酶的磁检测电路实现对SARS-CoV-2全基因组RNA的快速检测
Enja Laureen Rösch,Rebecca Sack,Mohammad Suman Chowdhury et al.
Enja Laureen Rösch et al.
Polymerase chain reaction (PCR) requires thermal cycling and enzymatic reactions for sequence amplification, hampering their applications in point-of-care (POC) settings. Magnetic bioassays based on magnetic particle spectroscopy (MPS) and ...
Probing Glycerolipid Metabolism using a Caged Clickable Glycerol-3-Phosphate Probe [0.03%]
使用笼型 clickable 甘油-3-磷酸探针探究甘油磷脂代谢
Jinchao Lou,Christelle F Ancajas,Yue Zhou et al.
Jinchao Lou et al.
In this study, we present the probe SATE-G3P-N3 as a novel tool for metabolic labeling of glycerolipids (GLs) to investigate lipid metabolism in yeast cells. By introducing a clickable azide handle onto the glycerol backbone, this probe ena...
Oligonucleotide-Based Photoaffinity Probes: Chemical Tools and Applications for Protein Labeling [0.03%]
基于寡核苷酸的光亲和探针：蛋白质标记的化学工具及应用
Carole Saintomé,Océane Monfret,Gilles Doisneau et al.
Carole Saintomé et al.
A variety of proteins interact with DNA and RNA, including polymerases, histones, ribosomes, transcription factors, and repair enzymes. However, the transient non-covalent nature of these interactions poses challenges for analysis. Introduc...
Using LanM Enzymes to Modify Glucagon-Like Peptides 1 and 2 in E.coli [0.03%]
利用LanM酶在大肠杆菌中修饰胰高血糖素样肽1和2
Camilla K Larsen,Peter Lindquist,Mette Rosenkilde et al.
Camilla K Larsen et al.
Selective modification of peptides is often exploited to improve pharmaceutically relevant properties of bioactive peptides like stability, circulation time, and potency. In Nature, natural products belonging to the class of ribosomally syn...
Caiqing Yuan,Fei Zhou,Zhihao Xu et al.
Caiqing Yuan et al.
Biomarkers are crucial physiological and pathological indicators in the host. Over the years, numerous detection methods have been developed for biomarkers, given their significant potential in various biological and biomedical applications...