Fibrinogen and Ceruloplasmin Modulate Neutrophils' Biomechanics and Motility [0.03%]
纤维蛋白原和细胞铜蓝蛋白调节中性粒细胞的生物力学和运动能力
Natalia Fedorova,Vasilii Kolmogorov,Sergey Gorelov et al.
Natalia Fedorova et al.
Neutrophils are the most abundant cells of the innate immune system, migrating from the bloodstream into tissues to the focus of inflammation. They are extremely sensitive to the microenvironment, including the composition of blood plasma. ...
A Native Nepenthesin Reactor for Improved Proteolytic Digestion of Intrinsically Disordered Proteins in Proteomics Workflows [0.03%]
一种用于改进质谱工作流程中内在无序蛋白酶解的原生茅膏菜蛋白酶反应器
Christian Wall,Frank Hause,Wiebke Grimm et al.
Christian Wall et al.
Intrinsically disordered proteins and proteins containing intrinsically disordered regions often harbor sequences that are difficult to digest with conventional proteases, such as trypsin, Asp-N, or pepsin. In particular, proline-rich regio...
Biocatalysis Under Reduced Pressure; Two-Step, One-Pot Amide Synthesis Using an Immobilised Transaminase/Lipase Cascade in Combination With By-Product Removal [0.03%]
减压生物催化；结合副产物去除的固定化转氨酶/脂肪酶级联反应的一锅两步法合成酰胺反应
Lisa Kennedy,Nicholas Mulholland,Andrew Gomm et al.
Lisa Kennedy et al.
Immobilisation of biocatalysts has expanded their applicability under non-conventional conditions, particularly for compatibility in synthetic processes by improving stability in non-aqueous media and enabling reusability. The combination o...
Advances in Biomolecular Condensates: From Disease Therapy to Synthetic Biology [0.03%]
生物分子凝聚体研究进展：从疾病治疗到合成生物学
Wenjun Xie,Chaojuan Yang,Mengqian Shi et al.
Wenjun Xie et al.
Biomolecular condensates (BMCs) are membraneless organelles (MLOs) formed by liquid-liquid phase separation (LLPS) of biomolecules such as proteins and nucleic acids (NAs). They are widely present in organisms and participate in key life pr...
Dan Li,Leif A Kirsebom,Roland K Hartmann
Dan Li
The RNA subunit of human nuclear RNase P, H1 RNA, has low catalytic activity in the absence of its protein cofactors. To improve H1 RNA activity, we introduced minor structural changes toward the bacterial consensus into its catalytic (C) d...
David Cullinane,Karmel Sofia Gkika,Surajit Ghosh et al.
David Cullinane et al.
Photoactive ruthenium polypyridyl complexes are intensively studied as imaging probes and (photo)therapeutics because of their attractive photophysical properties, including long-lived, oxygen-responsive luminescence, and their ability to s...
Fluorescence-Quenched Mycoloyl-Arabinofuranoside Analogs: Fluorogenic Probes for Mycobacterial Outer Membrane-Degrading Enzymes [0.03%]
荧光淬灭的分枝菌酸阿拉伯糖苷类似物：分枝杆菌外膜降解酶的荧光探针
Abdulai Zigli,Ulysses G Johnson,Douglas C Wing et al.
Abdulai Zigli et al.
Mycobacteria, including the tuberculosis pathogen Mycobacterium tuberculosis, are enclosed by a highly complex cell envelope with an outer membrane, or mycomembrane, which provides extraordinary protection from antibiotics and other stresse...
Bioactive and Antibacterial Gallic Acid Incorporated 3D-Printed Hydroxyapatite/Gelatin/Carboxymethyl Cellulose Scaffolds for Bone Tissue Engineering [0.03%]
一种载有棓酸的生物活性及抗菌性能的羟基磷灰石/明胶/羧甲基纤维素三维打印多孔支架用于骨组织工程
Alper Güven,Sezgi Iyigün,Emrah Torlak et al.
Alper Güven et al.
This study reports the development of 3D-printed gallic acid (GA) incorporated nano-hydroxyapatite /carboxymethyl cellulose/gelatin (GA/HAp/CMC/GEL) scaffolds for bone tissue engineering with combined bioactive and antibacterial functionali...
Generating Ultra-Fast Protein trans-Splicing of a Cysteine-Less and Semisynthetic Split Intein for Chemical Protein Labeling [0.03%]
用于化学蛋白质标记的不含半胱氨酸的半合成分裂intein的超快蛋白质跨剪接生成
Christoph Humberg,Tobias M E Terhorst,Tim Pasch et al.
Christoph Humberg et al.
Cysteine-less split inteins have recently emerged as valuable addition to the protein labeling and modification toolbox as they can perform the protein trans-splicing (PTS) reaction under oxidizing conditions. Furthermore, their use is comp...
Panagiotis Mougkogiannis,Andrew Adamatzky
Panagiotis Mougkogiannis
Proteinoid microspheres-abiotically synthesized by thermal polymerization of amino acids-exhibit spontaneous electrical potential fluctuations despite lacking genetic material, membranes, or ion channels. Here, we quantify the electrical an...