Zinc and copper effect mechanical cell adhesion properties of the amyloid precursor protein [0.03%]
锌和铜对淀粉样前体蛋白的机械细胞粘附性质的影响
Alexander August,Sabrina Hartmann,Sandra Schilling et al.
Alexander August et al.
The amyloid precursor protein (APP) can be modulated by the binding of copper and zinc ions. Both ions bind with low nanomolar affinities to both subdomains (E1 and E2) in the extracellular domain of APP. However, the impact of ion binding ...
Implications of TRPM3 and TRPM8 for sensory neuron sensitisation [0.03%]
瞬时受体电位锚channels 3和8对感觉神经元敏感化的影响
Marc Behrendt
Marc Behrendt
Sensory neurons serve to receive and transmit a wide range of information about the conditions of the world around us as well as the external and internal state of our body. Sensitisation of these nerve cells, i.e. becoming more sensitive t...
Karla L H Feijs-Žaja,Christina E Weinberg
Karla L H Feijs-Žaja
Editorial
Biological chemistry. 2024 Oct 7. DOI:10.1515/hsz-2024-0126 2024
A platform for the early selection of non-competitive antibody-fragments from yeast surface display libraries [0.03%]
从酵母展示文库中筛选非竞争性抗体片段的平台
Léxane Fournier,Deniz Demir,Desislava Elter et al.
Léxane Fournier et al.
In this work, we report the development of a platform for the early selection of non-competitive antibody-fragments against cell surface receptors that do not compete for binding of their natural ligand. For the isolation of such subtype of...
A tailored cytochrome P450 monooxygenase from Gordonia rubripertincta CWB2 for selective aliphatic monooxygenation [0.03%]
来源于Gordonia rubripertincta CWB2的定制细胞色素P450单加氧酶用于选择性烷基单氧官能化
Fabian Peter Josef Schultes,Leon Welter,Myra Schmidtke et al.
Fabian Peter Josef Schultes et al.
Cytochrome P450 monooxygenases are recognized as versatile biocatalysts due to their broad reaction capabilities. One important reaction is the hydroxylation of non-activated C-H bonds. The subfamily CYP153A is known for terminal hydroxylat...
Protein persulfidation in plants: mechanisms and functions beyond a simple stress response [0.03%]
植物蛋白质过硫化：不仅仅是应激反应的机制和功能超越简单压力响应的植物蛋白质过硫化的机制和功能
Anna Moseler,Stephan Wagner,Andreas J Meyer
Anna Moseler
Posttranslational modifications (PTMs) can modulate the activity, localization and interactions of proteins and (re)define their biological function. Understanding how changing environments can alter cellular processes thus requires detaile...
Simultaneous spectral illumination of microplates for high-throughput optogenetics and photobiology [0.03%]
用于高通量光遗传学和光生物学的微孔板同步光谱照射
Arend Vogt,Raik Paulat,Daniel Parthier et al.
Arend Vogt et al.
The biophysical characterization and engineering of optogenetic tools and photobiological systems has been hampered by the lack of efficient methods for spectral illumination of microplates for high-throughput analysis of action spectra. Cu...
The BCL11A transcription factor stimulates the enzymatic activities of the OGG1 DNA glycosylase [0.03%]
BCL11A转录因子可刺激OGG1 DNA糖基化酶的酶活性
Tetiana Petrachkova,Olha Soldatkina,Lam Leduy et al.
Tetiana Petrachkova et al.
The BCL11A transcription factor has previously been shown to interact with and stimulate the enzymatic activities of the NTHL1 DNA glycosylase and Pol β polymerase. Here we show that BCL11A and a smaller peptide encompassing amino acids 16...
Analysis of cell cycle stage, replicated DNA, and chromatin-associated proteins using high-throughput flow cytometry [0.03%]
基于高通量流式细胞术的细胞周期阶段、复制DNA和染色质相关蛋白质分析
Marina Bejarano Franco,Safia Boujataoui,Majd Hadji et al.
Marina Bejarano Franco et al.
Flow cytometry is a versatile tool used for cell sorting, DNA content imaging, and determining various cellular characteristics. With the possibility of high-throughput analyses, it combines convenient labelling techniques to serve rapid, q...
The TOM complex from an evolutionary perspective and the functions of TOMM70 [0.03%]
从进化角度解析TOM复合物及其TOMM70的功能
Metin Özdemir,Sven Dennerlein
Metin Özdemir
In humans, up to 1,500 mitochondrial precursor proteins are synthesized at cytosolic ribosomes and must be imported into the organelle. This is not only essential for mitochondrial but also for many cytosolic functions. The majority of mito...