Detection of a 7SL RNA-derived small non-coding RNA using Molecular Beacons in vitro and in cells [0.03%]
利用分子信标检测7SL RNA衍生的小非编码RNA
Nina Weigert,Anna-Lena Schweiger,Jonas Gross et al.
Nina Weigert et al.
Small non-coding RNAs (sncRNA) are involved in many steps of the gene expression cascade and regulate processing and expression of mRNAs by the formation of ribonucleoprotein complexes (RNP) such as the RNA-induced silencing complex (RISC)....
Rodent models for mood disorders - understanding molecular changes by investigating social behavior [0.03%]
用于情绪障碍的小鼠模型——通过研究社交行为来了解分子变化
Patrick R Reinhardt,Candy D C Theis,Georg Juckel et al.
Patrick R Reinhardt et al.
Mood disorders, including depressive and bipolar disorders, are the group of psychiatric disorders with the highest prevalence and disease burden. However, their pathophysiology remains poorly understood. Animal models are an extremely usef...
Characterization of the active site in the thiocyanate-forming protein from Thlaspi arvense (TaTFP) using EPR spectroscopy [0.03%]
利用电子顺磁共振光谱技术表征Thlaspi arvense中形成硫氰酸盐的蛋白质(TaTFP)的活性位点
Haleh Hashemi Haeri,Nicola Schneegans,Daniela Eisenschmidt-Bönn et al.
Haleh Hashemi Haeri et al.
Glucosinolates are plant thioglucosides, which act as chemical defenses. Upon tissue damage, their myrosinase-catalyzed hydrolysis yields aglucones that rearrange to toxic isothiocyanates. Specifier proteins such as thiocyanate-forming prot...
Intracellular spatially-targeted chemical chaperones increase native state stability of mutant SOD1 barrel [0.03%]
胞内定位型化学伴侣分子可提高突变SOD1 barrels的天然构象稳定性
Sara S Ribeiro,David Gnutt,Salome Azoulay-Ginsburg et al.
Sara S Ribeiro et al.
Amyotrophic lateral sclerosis (ALS) is a progressive neurological disorder with currently no cure. Central to the cellular dysfunction associated with this fatal proteinopathy is the accumulation of unfolded/misfolded superoxide dismutase 1...
Crosstalk between cholesterol and PIP2 in the regulation of Kv7.2/Kv7.3 channels [0.03%]
胆固醇与PIP2在Kv7.2/Kv7.3通道调节中的相互作用
Mayra Delgado-Ramírez,Ana Laura López-Serrano,Sergio Sánchez-Armass et al.
Mayra Delgado-Ramírez et al.
The activity of neuronal Kv7.2/Kv7.3 channels is critically dependent on PIP2 and finely modulated by cholesterol. Here, we report the crosstalk between cholesterol and PIP2 in the regulation of Kv7.2/Kv7.3 channels. Our results show that c...
Highlight: integrative structural biology of dynamic macromolecular assemblies [0.03%]
聚焦整合结构生物学：动态大分子组装体的结构生物学
Ralf Ficner
Ralf Ficner
Rodrigo Maldonado,Gernot Längst
Rodrigo Maldonado
Mammalian genomes are extensively transcribed, producing a large number of coding and non-coding transcripts. A large fraction of the nuclear RNAs is physically associated with chromatin, functioning in gene activation and silencing, shapin...
Michael Habeck
Michael Habeck
There is a growing interest in characterizing the structure and dynamics of large biomolecular assemblies and their interactions within the cellular environment. A diverse array of experimental techniques allows us to study biomolecular sys...
Synthesis of the ribosomal RNA precursor in human cells: mechanisms, factors and regulation [0.03%]
人类细胞中的核糖体RNA前体合成：机制、因子及调控
Julia L Daiß,Joachim Griesenbeck,Herbert Tschochner et al.
Julia L Daiß et al.
The ribosomal RNA precursor (pre-rRNA) comprises three of the four ribosomal RNAs and is synthesized by RNA polymerase (Pol) I. Here, we describe the mechanisms of Pol I transcription in human cells with a focus on recent insights gained fr...
Marieke Enders,Piotr Neumann,Achim Dickmanns et al.
Marieke Enders et al.
Splicing of precursor mRNAs is a hallmark of eukaryotic cells, performed by a huge macromolecular machine, the spliceosome. Four DEAH-box ATPases are essential components of the spliceosome, which play an important role in the spliceosome a...