Bovine ultralong CDR-H3 derived knob paratopes elicit potent TNF-α neutralization and enable the generation of novel adalimumab-based antibody architectures with augmented features [0.03%]
源自牛超长CDR-H3的knob结构表位产生强大的TNF-α中和并能够生成具有增强特征的新adalimumab抗体构型
Paul Arras,Jasmin Zimmermann,Britta Lipinski et al.
Paul Arras et al.
In this work we have generated cattle-derived chimeric ultralong CDR-H3 antibodies targeting tumor necrosis factor α (TNF-α) via immunization and yeast surface display. We identified one particular ultralong CDR-H3 paratope that potently ...
In-depth analysis of Gαs protein activity by probing different fluorescently labeled guanine nucleotides [0.03%]
通过检测不同荧光标记的鸟嘌呤核苷酸对Gαs蛋白活性进行深入分析
Anna Pepanian,Paul Sommerfeld,Furkan Ayberk Binbay et al.
Anna Pepanian et al.
G proteins are interacting partners of G protein-coupled receptors (GPCRs) in eukaryotic cells. Upon G protein activation, the ability of the Gα subunit to exchange GDP for GTP determines the intracellular signal transduction. Although var...
Better safe than sorry: dual targeting antibodies for cancer immunotherapy [0.03%]
防患于未然：双靶向抗体用于癌症免疫治疗
Katrin Schoenfeld,Julia Harwardt,Harald Kolmar
Katrin Schoenfeld
Antibody-based therapies are revolutionizing cancer treatment and experience a steady increase from preclinical and clinical pipelines to market share. While the clinical success of monoclonal antibodies is frequently limited by low respons...
TIF1γ and SMAD4 regulation in colorectal cancer: impact on cell proliferation and liver metastasis [0.03%]
TIF1γ和SMAD4在结肠癌中的调节作用：对细胞增殖和肝转移的影响
Yanhui Wu,Bin Yu,Xi Ai et al.
Yanhui Wu et al.
We investigated the effects of transcriptional intermediary factor 1γ (TIF1γ) and SMAD4 on the proliferation and liver metastasis of colorectal cancer (CRC) cells through knockdown of TIF1γ and/or SMAD4 and knockdown of TIF1γ and/or res...
Celastrol inhibits angiogenesis and the biological processes of MDA-MB-231 cells via the DEGS1/S1P signaling pathway [0.03%]
celastrol通过DEGS1/s1p信号通路抑制血管生成及MDA-MB-231细胞的生物学行为
Lulu Jia,Shengnan Zhu,Mingfei Zhu et al.
Lulu Jia et al.
Celastrol (Cel) shows potent antitumor activity in various experimental models. This study examined the relationship between Cel's antivascular and antitumor effects and sphingolipids. CCK-8 assay, transwell assay, Matrigel, PCR-array/RT-PC...
Highlight: Horizons in Neuroscience - Organoids, Optogenetics and Remote Control [0.03%]
聚焦:神经科学新视野-类器官、光遗传学及遥控技术
Rolf Heumann
Rolf Heumann
Visualization of the membrane surface and cytoskeleton of oligodendrocyte progenitor cell growth cones using a combination of scanning ion conductance and four times expansion microscopy [0.03%]
结合扫描离子电导和4倍膨胀显微镜可视化少突前体细胞生长锥的膜表面及细胞骨架
Annika Haak,Heiko M Lesslich,Irmgard D Dietzel
Annika Haak
Growth cones of oligodendrocyte progenitor cells (OPCs) are challenging to investigate with conventional light microscopy due to their small size. Especially substructures such as filopodia, lamellipodia and their underlying cytoskeleton ar...
Methionine restriction attenuates the migration and invasion of gastric cancer cells by inhibiting nuclear p65 translocation through TRIM47 [0.03%]
甲硫氨酸限制通过TRIM47抑制核p65转位从而减弱胃癌细胞迁移和侵袭能力
Lin Xin,Yi-Wu Yuan,Chen-Xi Liu et al.
Lin Xin et al.
The prevention and treatment of gastric cancer has been the focus and difficulty of medical research. We aimed to explore the mechanism of inhibiting migration and invasion of gastric cancer cells by methionine restriction (MR). The human g...
Heterogenous nuclear ribonucleoprotein D-like controls endothelial cell functions [0.03%]
异质性核糖核蛋白D类似物控制内皮细胞功能
Sandra Fischer,Chiara Lichtenthaeler,Anastasiya Stepanenko et al.
Sandra Fischer et al.
HnRNPs are ubiquitously expressed RNA-binding proteins, tightly controlling posttranscriptional gene regulation. Consequently, hnRNP networks are essential for cellular homeostasis and their dysregulation is associated with cancer and other...
Platelet-derived growth factor-stimulated pulmonary artery smooth muscle cells regulate pulmonary artery endothelial cell dysfunction through extracellular vesicle miR-409-5p [0.03%]
血小板源性生长因子刺激的肺动脉平滑肌细胞通过细胞外囊泡传递miR-409-5p调节肺动脉内皮细胞功能障碍
Jeongyeon Heo,Hara Kang
Jeongyeon Heo
Platelet-derived growth factor (PDGF)-induced changes in vascular smooth muscle cells (VSMCs) stimulate vascular remodeling, resulting in vascular diseases such as pulmonary arterial hypertension. VSMCs communicate with endothelial cells th...