Michelle Juknaviciute Laursen,Mathilde Roth,Poul Nissen et al.
Michelle Juknaviciute Laursen et al.
P4-ATPases are lipid flippases that maintain membrane phospholipid asymmetry by transporting specific phospholipids from the exoplasmic to the cytosolic leaflet, an essential process for membrane integrity, trafficking and signaling. Severa...
Impact of Time, Temperature, and Conformational Changes on the Characterization of Cardiotonic Steroid-Na+/K+-ATPase Interactions [0.03%]
时间和温度对糖皮质激素-Na+/K+-ATP酶相互作用表征的影响
Pedro Azalim-Neto,Leandro Barbosa,Cristoforo Scavone et al.
Pedro Azalim-Neto et al.
Cardiotonic steroids (CTS) have gained prominence due to various mechanisms of action proposed or demonstrated to explain their effects, including their known classical and non-classical signaling pathways mediated by Na+/K+-ATPase (NKA) bi...
In vitro Reconstitution of Syncytin-2 and MFSD2A Reveals their Functional Tethering in Membrane Fusion Initiation [0.03%]
体外重组Syncytin-2和MFSD2A并揭示它们在膜融合起始中的功能连接性
Zhan Xiang,Lu Xu,Ying Li et al.
Zhan Xiang et al.
Syncytin-2, a human endogenous retrovirus-derived fusogen, is essential for placental syncytiotrophoblast formation by mediating trophoblast cell-cell fusion. While Syncytin-2 shares structural similarities with viral envelope proteins, the...
Structural Basis of SERCA Inhibition by Derivatives of di-tert-butylhydroquinone Revealed by X-ray Crystallography [0.03%]
用于抑制SERCA的二叔丁基对苯二酚衍生物的晶体结构分析
Ryuta Kanai,Ayami Hirata,Chikashi Toyoshima et al.
Ryuta Kanai et al.
The small molecule di-tert-butylhydroquinone (BHQ) is a potent inhibitor of the sarco/endoplasmic reticulum Ca²⁺-ATPase (SERCA). It inhibits the enzyme by stabilizing it in its E2 conformation and thereby preventing the transition to the ...
Fungal Extracellular Vesicles are Recoverable Across Variable Ultracentrifugation Speeds but Display Species-specific Profiles of Sedimentation [0.03%]
variable ultracentrifugation速度下的真菌细胞外囊泡的可回收性及其沉淀特征的物种特异性分析
Luisa J Jozefowicz,Bárbara T Bezerra,Eva Veres et al.
Luisa J Jozefowicz et al.
Extracellular vesicles (EVs) are central components of fungal biology, yet their isolation commonly relies on ultracentrifugation protocols originally developed for mammalian systems. In Cryptococcus, most EVs carry the capsular polysacchar...
Polyproline Modulates Membrane Translocation of Arginine-Rich Cell-Penetrating Peptides: Insights from Molecular Dynamics Simulations [0.03%]
多脯氨酸调节精氨细胞穿透肽的膜转运：分子动力学模拟启示
Seungho Choe,Afira Mariam,Maheen Ejaz
Seungho Choe
Recent experiments have shown that incorporating polyproline segments into arginine (R)-rich cell-penetrating peptides (CPPs) enhances membrane penetration. Here, we employ molecular dynamics (MD) simulations combined with the weighted ense...
Peculiarities of Phosphatidylserine Externalization by Nano- and Microsecond Electric Pulses [0.03%]
纳秒和微秒电脉冲诱导的磷脂酰丝氨酸外翻的特殊性
Mantas Silkunas,Alayna Enos,Iurii Semenov et al.
Mantas Silkunas et al.
Phosphatidylserine (PS) externalization is a hallmark of apoptosis but can also be triggered within seconds by pulsed electric fields (PEFs). Whether PS reaches the outer leaflet by lateral migration through electropores or indirectly throu...
Protonation of Key Acidic Residues Reveals Binding Features of PCABs to Gastric H, K-ATPase [0.03%]
关键酸性残基的质子化揭示了PCAB与胃H,K-ATP酶的结合特征
Gerardo Zerbetto De Palma,Nicole T Cerf,Mónica R Montes
Gerardo Zerbetto De Palma
The group of inhibitors known as Potassium Competitive Acid Blockers (PCABs) has become one of the main topics of current research into reducing gastric H,K-ATPase activity. The design of novel PCABs relies on structure-based drug design st...
Electrostatic Interaction as a Key Modulator of Na+,K+-ATPase Function [0.03%]
静电作用作为Na+,K+-ATP酶功能的关键调节因素
Shadreen Fairuz,Zhitong Li,Amy Gorman et al.
Shadreen Fairuz et al.
The Na+,K+-ATPase is an integral membrane protein present in all animal plasma membranes. It uses the energy of ATP hydrolysis to pump 3 Na+ ions per ATP hydrolysed from the cell cytoplasm into the extracellular fluid in exchange for 2 K+ i...
Evolutionary Prevalence of the Electrostatic Switch Mechanism in P-type ATPases [0.03%]
P型ATP酶中静电开关机制的进化普适性
Nikita S Badve,Alyssa V Buda,Jonas A Káral et al.
Nikita S Badve et al.
P-type ATPases are active transporter enzymes that maintain ion electrochemical gradients for functions such as muscle contraction, kidney function, digestion, and nerve signalling. The dysfunction of these ATPases has been linked to severa...