Fluorescence-Gated Flow Cytometry Approach for Measuring Lipid Flippase Activity in Mammalian Cells [0.03%]
一种用于测量哺乳动物细胞中脂质翻转酶活性的荧光门控流式细胞术方法
Klara T Scholtissek,Chris Van den Haute,Filip K Pamula et al.
Klara T Scholtissek et al.
P4‑ATPase lipid flippases establish transbilayer lipid asymmetry in eukaryotic membranes, with increasing evidence indicating a role for interacting partners in regulating flippase activity. However, assessing interactor‑dependent effects...
Oxidation-Dependent Behavior of Saturated and Unsaturated Phosphatidylcholine Model Membranes: Insights from Langmuir Monolayers and TBARS Analysis [0.03%]
基于Langmuir单分子层和TBARS分析的饱和及不饱和卵磷脂模型膜的氧化依赖行为研究
Hoda Talo,Thad Harroun
Hoda Talo
Lipid oxidation plays an important role in modifying membrane organization and interfacial behavior, particularly in phospholipids containing unsaturated acyl chains. In this study, Langmuir monolayer measurements and thiobarbituric acid re...
Is the Bilayer Insertion of the Peptide KALP23 Under Kinetic or Thermodynamic Control? [0.03%]
肽KALP23的双层插入是受动力学控制还是热力学控制？
Alexander Kyrychenko,Alexey S Ladokhin
Alexander Kyrychenko
Replacing a protein's sidechain can alter its interaction with a membrane, conferring a thermodynamic benefit or penalty. These thermodynamic consequences of mutations can be estimated using hydrophobicity scales, yet are challenging to val...
K Magkakis,F Sabzian-Molaei,F Orädd et al.
K Magkakis et al.
Time-resolved X-ray solution scattering (TR-XSS) provides direct access to protein structural dynamics but has largely been restricted from the microsecond range up to approximately 100 milliseconds. As a result, slower enzymatic systems, i...
The Conserved Fusion Peptide Sequence of SARS-CoVs is Insensitive to Ca2+ in Inducing Lipid Mixing in Model Membranes [0.03%]
冠状病毒(SARS-CoV)保守的融合肽序列在诱导模型膜脂质混合时不受钙离子的影响
Priyanka Mahapatra,Hirak Chakraborty
Priyanka Mahapatra
The viral envelope fuses with the host cell membrane to initiate viral infection. In the case of SARS-CoVs, the fusion process is catalyzed by the spike (S) protein. The S1 subunit interacts with the host cell receptor and assists in dockin...
Michelle Juknaviciute Laursen,Mathilde Roth,Poul Nissen et al.
Michelle Juknaviciute Laursen et al.
P4-ATPases are lipid flippases that maintain membrane phospholipid asymmetry by transporting specific phospholipids from the exoplasmic to the cytosolic leaflet, an essential process for membrane integrity, trafficking and signaling. Severa...
Impact of Time, Temperature, and Conformational Changes on the Characterization of Cardiotonic Steroid-Na+/K+-ATPase Interactions [0.03%]
时间和温度对糖皮质激素-Na+/K+-ATP酶相互作用表征的影响
Pedro Azalim-Neto,Leandro Barbosa,Cristoforo Scavone et al.
Pedro Azalim-Neto et al.
Cardiotonic steroids (CTS) have gained prominence due to various mechanisms of action proposed or demonstrated to explain their effects, including their known classical and non-classical signaling pathways mediated by Na+/K+-ATPase (NKA) bi...
In vitro Reconstitution of Syncytin-2 and MFSD2A Reveals their Functional Tethering in Membrane Fusion Initiation [0.03%]
体外重组Syncytin-2和MFSD2A并揭示它们在膜融合起始中的功能连接性
Zhan Xiang,Lu Xu,Ying Li et al.
Zhan Xiang et al.
Syncytin-2, a human endogenous retrovirus-derived fusogen, is essential for placental syncytiotrophoblast formation by mediating trophoblast cell-cell fusion. While Syncytin-2 shares structural similarities with viral envelope proteins, the...
Structural Basis of SERCA Inhibition by Derivatives of di-tert-butylhydroquinone Revealed by X-ray Crystallography [0.03%]
用于抑制SERCA的二叔丁基对苯二酚衍生物的晶体结构分析
Ryuta Kanai,Ayami Hirata,Chikashi Toyoshima et al.
Ryuta Kanai et al.
The small molecule di-tert-butylhydroquinone (BHQ) is a potent inhibitor of the sarco/endoplasmic reticulum Ca²⁺-ATPase (SERCA). It inhibits the enzyme by stabilizing it in its E2 conformation and thereby preventing the transition to the ...
Fungal Extracellular Vesicles are Recoverable Across Variable Ultracentrifugation Speeds but Display Species-specific Profiles of Sedimentation [0.03%]
variable ultracentrifugation速度下的真菌细胞外囊泡的可回收性及其沉淀特征的物种特异性分析
Luisa J Jozefowicz,Bárbara T Bezerra,Eva Veres et al.
Luisa J Jozefowicz et al.
Extracellular vesicles (EVs) are central components of fungal biology, yet their isolation commonly relies on ultracentrifugation protocols originally developed for mammalian systems. In Cryptococcus, most EVs carry the capsular polysacchar...