In sample pH measurement by 31P phosphate NMR: application to measure the intrinsic GTPase activity of Rab1a [0.03%]
利用31P磷酸核磁共振进行样品内PH值测定及测量Rab1a固有GTP酶活性的应用
David Pantoja-Uceda,Douglas V Laurents
David Pantoja-Uceda
A quick, noninvasive method based on 31P NMR spectroscopy to measure the pH of samples containing phosphate buffer is presented. By taking advantage of all four phosphate species populated at acidic, mildly acidic, mildly alkaline and alkal...
The MOlecular-Scale Biophysics Research Infrastructure (MOSBRI) Project and its Outcomes [0.03%]
分子尺度生物物理研究基础设施(MOSBRI)项目及其成果
Gregor Anderluh,Ornella Cavalleri,Patrick England et al.
Gregor Anderluh et al.
Bitter taste TAS2R14 and TAS2R46 receptors bound to G proteins: comparison of cryo-EM, AlphaFold, and molecular dynamics structures [0.03%]
苦味TAS2R14和TAS2R46受体结合G蛋白：基于cryo-EM、AlphaFold及分子动力学模型的结构分析
Ruth Pachter,Soo-Kyung Kim,Yixin C Xu et al.
Ruth Pachter et al.
Bitter taste type 2 G protein-coupled receptors (TAS2R GPCRs) detect bitter substances on the tongue, but they are also prevalent in tissues throughout the human body that play a role in cancer and other diseases, making them targets for ph...
Homologous series of N-acylmelatonins: synthesis, biophysical studies, enhanced antioxidant, antimicrobial and anticancer activities [0.03%]
N-酰基-甲基化衍生的褪黑素同系物系列：合成、生物物理研究及增强型抗氧化、抗微生物和抗癌活性
Surendra Pothuraju,Arya Jyothibabu Sajila,Ravi Kanth Kamlekar
Surendra Pothuraju
N-acylmelatonins (NAMs), a class of fatty acid amides featuring melatonin as the polar head group, demonstrate significant biomedical potential. In this study, a homologous series of saturated NAMs (n = 9-18) were synthesized and fully char...
Synthesis and biophysical characterization of bioengineered cytochrome nanowires [0.03%]
生物工程细胞色素纳米线的合成与生物物理性质表征
M Raquel Pacheco,Ana J Carvalho,Marta A Silva et al.
M Raquel Pacheco et al.
Geobacter bacteria produce multiheme c-type cytochrome nanowires that are involved in long-range extracellular electron transfer. The ability of these protein nanowires to conduct electrical current makes them promising candidates for elect...
Tracking the rotational dynamics of Chlamydomonas reinhardtii across culture days using optical tweezers [0.03%]
基于光学钳技术利用光镊追踪培养液中不同天数的蛋白核小球藻细胞旋转动力学行为
Shurui Deng,Yanshan Lin,Domna G Kotsifaki
Shurui Deng
The green alga Chlamydomonas reinhardtii (CR) has been explored as the live component of biohybrid microrobots due to its intrinsic motility. However, cell performance depends on physiological state, which evolves with culture age. Using op...
Interaction of Pf4 tactoids with bacteria and synthetic colloidal rods [0.03%]
Pf4 táctoid与细菌和合成胶体棒的相互作用
Mariana de Oliveira Silva,Iago Grobas,Abul K Tarafder et al.
Mariana de Oliveira Silva et al.
We investigated the interaction of liquid crystal droplets, formed by a phase separating system of elongated virus particles and polymer with rod-like impurities. The virus particles were Pf4, which when mixed with sodium alginate as polyme...
Dynamics of viral self-assembly, viral genome packaging, and virus-cell interactions studied by optical tweezers [0.03%]
运用光学镊子研究病毒自我装配、基因组包装及与细胞相互作用的动态过程
Yanping Gong,Dorus T Harmsen,Wouter H Roos
Yanping Gong
Viruses are complex supramolecular assemblies that propagate their genetic material from cell to cell, thereby relying on host cell mechanisms. Employing a combination of passive and active strategies, they efficiently package, transport an...
Process-analytical study of IL-6 refolding during batch buffer exchange [0.03%]
批量缓冲液交换过程中IL-6重折叠的过程分析研究
Yuichi Nakahara,Yuta Endo,Yutaka Matsuda et al.
Yuichi Nakahara et al.
Statistical mechanics of soft collagenous tissues: a constitutive model with damage for cerebral bridging veins [0.03%]
软胶原组织的统计力学：脑桥静脉损伤的本构模型
Silvia García-Vilana,David Sánchez-Molina,Hamed Abdi et al.
Silvia García-Vilana et al.