The ADP-ribosylating thermozyme from Sulfolobus solfataricus is a DING protein [0.03%]
来自Sulfolobus solfataricus的ADP核糖基化热酶是一种DING蛋白
Antimo Di Maro,Anna De Maio,Sabrina Castellano et al.
Antimo Di Maro et al.
The partial amino acid sequence of the sulfolobal thermoprotein biochemically characterized as poly(ADP-ribose)polymerase-like enzyme overlaps those of DING proteins. This group of proteins, widely occurring in animals, plants and eubacteri...
Impact of detergents on the activity of acetylcholinesterase and on the effectiveness of its inhibitors [0.03%]
表面活性剂对乙酰胆碱酯酶活性及抑制剂作用效果的影响
Martina Zimmermann,Martin S Westwell,Susan A Greenfield
Martina Zimmermann
Acetylcholinesterase (AChE) plays a central role in the development of Alzheimer's disease: AChE inhibition for preventing the characteristic dwindling of acetylcholine levels constitutes the current standard treatment for the disorder. Amo...
Conformational changes in bovine lactoferrin induced by slow or fast temperature increases [0.03%]
缓慢或快速升温引起的牛乳铁蛋白构象变化
Waleska D Schwarcz,Lorena Carnelocce,Jerson L Silva et al.
Waleska D Schwarcz et al.
Lactoferrin (LF) is an iron-binding protein present in several secreted substances, such as milk, and has broad antimicrobial and physiological properties. Because high temperatures may affect protein stability and its functional properties...
Contribution of the C30/C75 disulfide bond to the biological properties of onconase [0.03%]
半胱氨酸30/半胱氨酸75二硫键对onconase的生物学特性的影响研究
Gerard Torrent,Antoni Benito,Jessica Castro et al.
Gerard Torrent et al.
Onconase, a member of the pancreatic type ribonuclease family, is currently used as a chemotherapeutic agent for the treatment of different types of cancer. It is widely accepted that one of the properties that renders this enzyme cytotoxic...
Emmanuel Godat,Virginie Hervé-Grvépinet,Florian Veillard et al.
Emmanuel Godat et al.
Although cysteine cathepsins, including cathepsin K, are sensitive to oxidation, proteolytically active forms are found at inflammatory sites. Regulation of cathepsin K activity was analyzed in the presence of H2O2 to gain an insight into t...
In vivo analysis reveals substrate-gating mutants of a rhomboid intramembrane protease display increased activity in living cells [0.03%]
体内分析揭示类风湿 intramembrane 蛋白酶的底物门控突变体在活细胞中表现出更高的活性
Sinisa Urban,Rosanna P Baker
Sinisa Urban
Intramembrane proteases hydrolyze peptide bonds within cell membranes. Recent crystal structures revealed that rhomboid intramembrane proteases contain a hydrated active site that opens to the outside of the cell, but is protected laterally...
Substrate specificity determination of mouse implantation serine proteinase and human kallikrein-related peptidase 6 by phage display [0.03%]
噬菌体展示技术测定小鼠着床丝氨酸蛋白酶和人相关激肽原酶6的底物特异性
Navneet Sharma,Katerina Oikonomopoulou,Kenichi Ito et al.
Navneet Sharma et al.
We constructed a random library of hexapeptides displayed on the surface of bacteriophage T7 to determine the substrate specificity of proteinases. The phage-displayed library was subjected to repeated rounds of biopanning with native impla...
Intestine-specific expression of green fluorescent protein-tagged cathepsin B: proof-of-principle experiments [0.03%]
绿色荧光蛋白标记的组织蛋白酶B的肠特异性表达：原理证明实验
Kristina Mayer,Maria E Iolyeva,Ulf Meyer-Grahle et al.
Kristina Mayer et al.
We hypothesized that tissue-specific expression of cathepsin B-enhanced green fluorescent protein (CB-EGFP) can be driven by the A33-antigen promoter that contains positive cis-regulatory elements, including caudal-related homeobox (CDX) bi...
Metastasis-associated C4.4A, a GPI-anchored protein cleaved by ADAM10 and ADAM17 [0.03%]
与转移相关的C4.4A是一种由ADAM10和ADAM17切割的糖基磷脂酰肌醇锚定蛋白
Cary W Esselens,Jordi Malapeira,Núria Colomé et al.
Cary W Esselens et al.
Metalloproteases play a complex role in tumor progression. While the activity of some ADAM, ADAMTS and matrix metalloproteases (MMPs) seems to be protumorigenic, the activity of others seems to prevent tumor progression. The identification ...
Isoaspartate-containing amyloid precursor protein-derived peptides alter efficacy and specificity of potential beta-secretases [0.03%]
异天门冬氨酸修饰的β分泌酶切割的淀粉样前体蛋白衍生肽改变潜在β分泌酶的功效和特异性
Livia Böhme,Torsten Hoffmann,Susanne Manhart et al.
Livia Böhme et al.
Neuritic plaques of Alzheimer patients are composed of multiple protein components. Among them, the amyloid beta-peptides (Abeta) 1-40/42 and further N- and C-terminally modified fragments of Abeta are highly abundant. Most prominent are th...